| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Uva e Vinho. |
|
Data corrente: |
16/11/2001 |
|
Data da última atualização: |
14/01/2019 |
|
Autoria: |
FILGUEIRAS, H. A. C. |
|
Título: |
Manga: pós-colheita. |
|
Ano de publicação: |
2000 |
|
Fonte/Imprenta: |
Brasília, DF: Embrapa Comunicação para Transferência de Tecnologia; Fortaleza: Embrapa Agroindústria Tropical, 2000. |
|
Páginas: |
40 p. |
|
Descrição Física: |
il. |
|
Série: |
(Frutas do Brasil, 2). |
|
Idioma: |
Português |
|
Palavras-Chave: |
Brasil. |
|
Thesagro: |
Colheita; Exportação; Fruticultura; Manga; Pós-Colheita. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 00558nam a2200193 a 4500
001 1538733
005 2019-01-14
008 2000 bl uuuu u0uu1 u #d
100 1 $aFILGUEIRAS, H. A. C.
245 $aManga$bpós-colheita.
260 $aBrasília, DF: Embrapa Comunicação para Transferência de Tecnologia; Fortaleza: Embrapa Agroindústria Tropical$c2000
300 $a40 p.$cil.
490 $a(Frutas do Brasil, 2).
650 $aColheita
650 $aExportação
650 $aFruticultura
650 $aManga
650 $aPós-Colheita
653 $aBrasil
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Uva e Vinho (CNPUV) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Agroenergia. |
|
Data corrente: |
03/09/2021 |
|
Data da última atualização: |
03/09/2021 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
A - 2 |
|
Autoria: |
PAES, B. G.; STEINDORF, A. S.; FORMIGHIERI, E. F.; PEREIRA, I. S.; ALMEIDA, J. R. M. de. |
|
Afiliação: |
BARBARA G. PAES, Universidade de Brasília; ANDREI STECCA STEINDORF, Universidade de Brasília; EDUARDO FERNANDES FORMIGHIERI, CNPAE; ILDINETE SILVA PEREIRA, Universidade de Brasília; JOAO RICARDO MOREIRA DE ALMEIDA, CNPAE. |
|
Título: |
Physiological characterization and transcriptome analysis of Pichia pastoris reveals its response to lignocellulose-derived inhibitors. |
|
Ano de publicação: |
2021 |
|
Fonte/Imprenta: |
AMB Express, v. 11, n. 2, 2021. |
|
Descrição Física: |
PDF: il. color. |
|
DOI: |
https://doi.org/10.1186/s13568-020-01170-9 |
|
Idioma: |
Inglês |
|
Conteúdo: |
The negative efects of lignocellulose-derived inhibitors such as acetic acid and furaldehydes on microbial metabolism constitute a signifcant drawback to the usage of biomass feedstocks for the production of fuels and chemicals. The yeast Pichia pastoris has shown a great biotechnological potential for producing heterologous proteins and renewable chemicals. Despite its relevance, the performance of P. pastoris in presence of lignocellulose-derived inhibitors remains unclear. In this work, our results show for the frst time the dose-dependent response of P. pastoris to acetic acid, furaldehydes (HMF and furfural), and sugarcane biomass hydrolysate, both at physiological and transcriptional levels. The yeast was able to grow in synthetic media with up to 6 g.L-1 acetic acid, 1.75 g.L-1 furaldehydes or hydrolysate diluted to 10% (v/v). However, its metabolism was completely hindered in presence of hydrolysate diluted to 30% (v/v). Additionally, the yeast was capable to co-consume acetic acid and glucose. At the transcriptional level, P. pastoris response to lignocellulose-derived inhibitors relays on the up-regulation of genes related to transmembrane transport, oxidoreductase activities, RNA processing, and the repression of pathways related to biosynthetic processes and central carbon metabolism. These results demonstrate a polygenetic response that involves detoxifcation activi ties, and maintenance of energy and cellular homeostasis. In this context, ALD4, OYE3, QOR2, NTL100, YCT1, and PPR1 were identifed as target genes to improve P. pastoris tolerance. Altogether, this work provides valuable insights into the P. pastoris stress tolerance, which can be useful to expand its use in diferent bioprocesses. MenosThe negative efects of lignocellulose-derived inhibitors such as acetic acid and furaldehydes on microbial metabolism constitute a signifcant drawback to the usage of biomass feedstocks for the production of fuels and chemicals. The yeast Pichia pastoris has shown a great biotechnological potential for producing heterologous proteins and renewable chemicals. Despite its relevance, the performance of P. pastoris in presence of lignocellulose-derived inhibitors remains unclear. In this work, our results show for the frst time the dose-dependent response of P. pastoris to acetic acid, furaldehydes (HMF and furfural), and sugarcane biomass hydrolysate, both at physiological and transcriptional levels. The yeast was able to grow in synthetic media with up to 6 g.L-1 acetic acid, 1.75 g.L-1 furaldehydes or hydrolysate diluted to 10% (v/v). However, its metabolism was completely hindered in presence of hydrolysate diluted to 30% (v/v). Additionally, the yeast was capable to co-consume acetic acid and glucose. At the transcriptional level, P. pastoris response to lignocellulose-derived inhibitors relays on the up-regulation of genes related to transmembrane transport, oxidoreductase activities, RNA processing, and the repression of pathways related to biosynthetic processes and central carbon metabolism. These results demonstrate a polygenetic response that involves detoxifcation activi ties, and maintenance of energy and cellular homeostasis. In this context, ALD4, OYE3, QOR2, NTL1... Mostrar Tudo |
|
Palavras-Chave: |
Furaldehydes; Komagataella phaffi; Lignocellulosic hydrolysate. |
|
Thesaurus NAL: |
Acetic acid; Pichia pastoris; Stress tolerance. |
|
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/225706/1/Physiological-characterization-and-transcriptome-analysis-2021.pdf
|
|
Marc: |
LEADER 02579naa a2200265 a 4500
001 2134061
005 2021-09-03
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1186/s13568-020-01170-9$2DOI
100 1 $aPAES, B. G.
245 $aPhysiological characterization and transcriptome analysis of Pichia pastoris reveals its response to lignocellulose-derived inhibitors.$h[electronic resource]
260 $c2021
300 $cPDF: il. color.
520 $aThe negative efects of lignocellulose-derived inhibitors such as acetic acid and furaldehydes on microbial metabolism constitute a signifcant drawback to the usage of biomass feedstocks for the production of fuels and chemicals. The yeast Pichia pastoris has shown a great biotechnological potential for producing heterologous proteins and renewable chemicals. Despite its relevance, the performance of P. pastoris in presence of lignocellulose-derived inhibitors remains unclear. In this work, our results show for the frst time the dose-dependent response of P. pastoris to acetic acid, furaldehydes (HMF and furfural), and sugarcane biomass hydrolysate, both at physiological and transcriptional levels. The yeast was able to grow in synthetic media with up to 6 g.L-1 acetic acid, 1.75 g.L-1 furaldehydes or hydrolysate diluted to 10% (v/v). However, its metabolism was completely hindered in presence of hydrolysate diluted to 30% (v/v). Additionally, the yeast was capable to co-consume acetic acid and glucose. At the transcriptional level, P. pastoris response to lignocellulose-derived inhibitors relays on the up-regulation of genes related to transmembrane transport, oxidoreductase activities, RNA processing, and the repression of pathways related to biosynthetic processes and central carbon metabolism. These results demonstrate a polygenetic response that involves detoxifcation activi ties, and maintenance of energy and cellular homeostasis. In this context, ALD4, OYE3, QOR2, NTL100, YCT1, and PPR1 were identifed as target genes to improve P. pastoris tolerance. Altogether, this work provides valuable insights into the P. pastoris stress tolerance, which can be useful to expand its use in diferent bioprocesses.
650 $aAcetic acid
650 $aPichia pastoris
650 $aStress tolerance
653 $aFuraldehydes
653 $aKomagataella phaffi
653 $aLignocellulosic hydrolysate
700 1 $aSTEINDORF, A. S.
700 1 $aFORMIGHIERI, E. F.
700 1 $aPEREIRA, I. S.
700 1 $aALMEIDA, J. R. M. de
773 $tAMB Express$gv. 11, n. 2, 2021.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Agroenergia (CNPAE) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Expressão de busca inválida. Verifique!!! |
|
|
