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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
09/01/2019 |
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Data da última atualização: |
12/02/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
VASCONCELOS, M. J. V. de; ANTUNES, M. S.; OLIVEIRA, M. F. de; LOPES, M. A.; FIGUEIREDO, J. E. F. |
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Afiliação: |
MARIA JOSE VILACA DE VASCONCELOS, CNPMS; MAURICIO SCHUSTERSCHITZ ANTUNES, Colorado State University; MAURILIO FERNANDES DE OLIVEIRA, CNPMS; MAURICIO ANTONIO LOPES, DE/PR; JOSE EDSON FONTES FIGUEIREDO, CNPMS. |
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Título: |
Callus induction and plant regeneration from immature embryos culture of tropical maize. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Revista Brasileira de Milho e Sorgo, Sete Lagoas, v. 17, n. 3, p. 359-368, 2018. |
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Idioma: |
Inglês |
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Conteúdo: |
The development of protocols to overcome the current limitations of callus induction and in vitro regeneration of highly recalcitrant tropical maize is crucial for plant genetic transformation. The ability of embryogenic callus (EC) formation of 46 tropical maize hybrids and 14 inbred lines was tested using N6 medium with the following changes: medium A (N6 + 15 µM dicamba + 25 mM L-proline + 88 mM AgNO3), medium B (N6 + 30 µM dicamba + 25 mM L-proline + 88 mM AgNO3), medium C (N6+ 30 µM dicamba + 6 mM L-proline), and medium D (N6+ 30 µM dicamba + 25 mM L-proline). Compact (Type I) and friable (Type II) callus were induced in the four media. Twenty genotypes produced callus in all media, and four genotypes (CO32, AG8012, CMS477BC4F2, and CMS-HGZ10) produced the highest number of callus (114, 134, 131, and 126 calli, respectively). All immature embryos of ten genotypes produced callus in at least one medium, while eight genotypes were highly recalcitrant and they did not produce any callus. The frequencies of EC ranged from 0% to 38%, and the highest rate of EC was observed on medium B (0.40) with a total of 865 calli, and the lowest induction rate was obtained with medium D (0.29) with 555 calli (P= 0.05). From the seventy-two EC of 26 genotypes transferred to Murashige & Skoog regeneration medium, twenty-four (66.7%) differentiated into green plants which produced seeds in R0 and R1 generations, and twelve (33.3%) developed into albino plants. The results demonstrated that the problem of the recalcitrant genotypes can be, at least partially, overcome by using immature embryos as explants together with tissue culture media formulations. Keywords: Zea mays, immature embryos, somatic embryogenesis, tissue culture, type I and type II calli. MenosThe development of protocols to overcome the current limitations of callus induction and in vitro regeneration of highly recalcitrant tropical maize is crucial for plant genetic transformation. The ability of embryogenic callus (EC) formation of 46 tropical maize hybrids and 14 inbred lines was tested using N6 medium with the following changes: medium A (N6 + 15 µM dicamba + 25 mM L-proline + 88 mM AgNO3), medium B (N6 + 30 µM dicamba + 25 mM L-proline + 88 mM AgNO3), medium C (N6+ 30 µM dicamba + 6 mM L-proline), and medium D (N6+ 30 µM dicamba + 25 mM L-proline). Compact (Type I) and friable (Type II) callus were induced in the four media. Twenty genotypes produced callus in all media, and four genotypes (CO32, AG8012, CMS477BC4F2, and CMS-HGZ10) produced the highest number of callus (114, 134, 131, and 126 calli, respectively). All immature embryos of ten genotypes produced callus in at least one medium, while eight genotypes were highly recalcitrant and they did not produce any callus. The frequencies of EC ranged from 0% to 38%, and the highest rate of EC was observed on medium B (0.40) with a total of 865 calli, and the lowest induction rate was obtained with medium D (0.29) with 555 calli (P= 0.05). From the seventy-two EC of 26 genotypes transferred to Murashige & Skoog regeneration medium, twenty-four (66.7%) differentiated into green plants which produced seeds in R0 and R1 generations, and twelve (33.3%) developed into albino plants. The results demonstrated that ... Mostrar Tudo |
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Palavras-Chave: |
Calos tipo I e tipo II; Embriogênese somática. |
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Thesagro: |
Cultura de Tecido; Embrião Vegetal; Zea Mays. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/190064/1/Callus-induction.pdf
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Marc: |
LEADER 02500naa a2200229 a 4500
001 2103395
005 2019-02-12
008 2018 bl uuuu u00u1 u #d
100 1 $aVASCONCELOS, M. J. V. de
245 $aCallus induction and plant regeneration from immature embryos culture of tropical maize.$h[electronic resource]
260 $c2018
520 $aThe development of protocols to overcome the current limitations of callus induction and in vitro regeneration of highly recalcitrant tropical maize is crucial for plant genetic transformation. The ability of embryogenic callus (EC) formation of 46 tropical maize hybrids and 14 inbred lines was tested using N6 medium with the following changes: medium A (N6 + 15 µM dicamba + 25 mM L-proline + 88 mM AgNO3), medium B (N6 + 30 µM dicamba + 25 mM L-proline + 88 mM AgNO3), medium C (N6+ 30 µM dicamba + 6 mM L-proline), and medium D (N6+ 30 µM dicamba + 25 mM L-proline). Compact (Type I) and friable (Type II) callus were induced in the four media. Twenty genotypes produced callus in all media, and four genotypes (CO32, AG8012, CMS477BC4F2, and CMS-HGZ10) produced the highest number of callus (114, 134, 131, and 126 calli, respectively). All immature embryos of ten genotypes produced callus in at least one medium, while eight genotypes were highly recalcitrant and they did not produce any callus. The frequencies of EC ranged from 0% to 38%, and the highest rate of EC was observed on medium B (0.40) with a total of 865 calli, and the lowest induction rate was obtained with medium D (0.29) with 555 calli (P= 0.05). From the seventy-two EC of 26 genotypes transferred to Murashige & Skoog regeneration medium, twenty-four (66.7%) differentiated into green plants which produced seeds in R0 and R1 generations, and twelve (33.3%) developed into albino plants. The results demonstrated that the problem of the recalcitrant genotypes can be, at least partially, overcome by using immature embryos as explants together with tissue culture media formulations. Keywords: Zea mays, immature embryos, somatic embryogenesis, tissue culture, type I and type II calli.
650 $aCultura de Tecido
650 $aEmbrião Vegetal
650 $aZea Mays
653 $aCalos tipo I e tipo II
653 $aEmbriogênese somática
700 1 $aANTUNES, M. S.
700 1 $aOLIVEIRA, M. F. de
700 1 $aLOPES, M. A.
700 1 $aFIGUEIREDO, J. E. F.
773 $tRevista Brasileira de Milho e Sorgo, Sete Lagoas$gv. 17, n. 3, p. 359-368, 2018.
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Embrapa Milho e Sorgo (CNPMS) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
28/11/2018 |
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Data da última atualização: |
10/04/2024 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
REIS, P. R. C.; SILVA, J. C. J.; PASSOS, L. P. |
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Afiliação: |
Paola R. C. Reis, UFJF; Júlio C. J. Silva, UFJF; LEONIDAS PAIXAO PASSOS, CNPGL. |
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Título: |
Determinação de Al, Cr, Cu e Zn em amostras de cana-de-açúcar usando amostragem direta de suspenção e detecção por GF AAS. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
In: ENCONTRO NACIONAL DE QUÍMICA ANALÍTICA, 19.; CONGRESSO IBERO-AMERICANO DE QUÍMICA ANALÍTICA, 7., 2018, Caldas Novas, GO. Inovação para a química analítica sustentável: livro de resumos... São Paulo: Sociedade Brasileira de Química, 2018. |
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Idioma: |
Português |
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Palavras-Chave: |
Detecção rápida; Micronutrientes. |
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Thesagro: |
Espectrometria. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 00728nam a2200157 a 4500
001 2100238
005 2024-04-10
008 2018 bl uuuu u00u1 u #d
100 1 $aREIS, P. R. C.
245 $aDeterminação de Al, Cr, Cu e Zn em amostras de cana-de-açúcar usando amostragem direta de suspenção e detecção por GF AAS.
260 $aIn: ENCONTRO NACIONAL DE QUÍMICA ANALÍTICA, 19.; CONGRESSO IBERO-AMERICANO DE QUÍMICA ANALÍTICA, 7., 2018, Caldas Novas, GO. Inovação para a química analítica sustentável: livro de resumos... São Paulo: Sociedade Brasileira de Química$c2018
650 $aEspectrometria
653 $aDetecção rápida
653 $aMicronutrientes
700 1 $aSILVA, J. C. J.
700 1 $aPASSOS, L. P.
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