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Registro Completo |
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Biblioteca(s): |
Embrapa Trigo. |
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Data corrente: |
10/01/2010 |
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Data da última atualização: |
15/03/2016 |
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Autoria: |
FEUILLET, C.; MESSMER, M.; SCHACHERMAYR, G.; KELLER, B. |
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Título: |
Genetic and physical characterization of the LR1 leaf rust resistance locus in wheat (Triticum aestivum L.). |
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Ano de publicação: |
1995 |
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Fonte/Imprenta: |
Molecular and General Genetics, v. 248, p. 553-562, 1995. |
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Idioma: |
Inglês |
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Conteúdo: |
The objective of this study was to characterize the leaf rust resistance locus Lr1 in wheat. Restriction fragment length polymorphism (RFLP) analysis was performed on the resistant line Lr1/6* Thatcher and the susceptible varieties Thatcher and Frisal, as well as on the segregating F2 populations. Seventeen out of 37 RFLP probes mapping to group 5 chromosomes showed polymorphism between Lr1/6* Thatcher and Frisal, whereas 11 probes were polymorphic between the near-isogenic lines (NILs) Lr1/6* Thatcher and Thatcher. Three of these probes were linked to the resistance gene in the segregating F2 populations. One probe (pTAG621) showed very tight linkage to Lr1 and mapped to a single-copy region on chromosome 5D. The map location of pTAG621 at the end of the long arm of chromosome 5D was confirmed by the absence of the band in the nulli-tetrasomic line N5DT5B of Chinese Spring and a set of deletion lines of Chinese Spring lacking the distal part of 5DL. Twenty-seven breeding lines containing the Lr1 resistance gene in different genetic backgrounds showed the same band as Lr1/6* Thatcher when hybridized with pTAG621. The RFLP marker was converted to a sequence-tagged-site marker using polymerase chain reaction (PCR) amplification. Sequencing of the specific fragment amplified from both NILs revealed point mutations as well as small insertion/deletion events. These were used to design primers that allowed amplification of a specific product only from the resistant line Lr1/6* Thatcher. This STS, specific for the Lr1 resistance gene, will allow efficient selection for the disease resistance gene in wheat breeding programmes. In addition, the identification of a D-genome-specific probe tightly linked to Lr1 should ultimately provide the basis for positional cloning of the gene. MenosThe objective of this study was to characterize the leaf rust resistance locus Lr1 in wheat. Restriction fragment length polymorphism (RFLP) analysis was performed on the resistant line Lr1/6* Thatcher and the susceptible varieties Thatcher and Frisal, as well as on the segregating F2 populations. Seventeen out of 37 RFLP probes mapping to group 5 chromosomes showed polymorphism between Lr1/6* Thatcher and Frisal, whereas 11 probes were polymorphic between the near-isogenic lines (NILs) Lr1/6* Thatcher and Thatcher. Three of these probes were linked to the resistance gene in the segregating F2 populations. One probe (pTAG621) showed very tight linkage to Lr1 and mapped to a single-copy region on chromosome 5D. The map location of pTAG621 at the end of the long arm of chromosome 5D was confirmed by the absence of the band in the nulli-tetrasomic line N5DT5B of Chinese Spring and a set of deletion lines of Chinese Spring lacking the distal part of 5DL. Twenty-seven breeding lines containing the Lr1 resistance gene in different genetic backgrounds showed the same band as Lr1/6* Thatcher when hybridized with pTAG621. The RFLP marker was converted to a sequence-tagged-site marker using polymerase chain reaction (PCR) amplification. Sequencing of the specific fragment amplified from both NILs revealed point mutations as well as small insertion/deletion events. These were used to design primers that allowed amplification of a specific product only from the resistant line Lr1/6* Tha... Mostrar Tudo |
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Palavras-Chave: |
Ferrugem da folha; Gene de resistência; PCR; RFLP. |
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Thesagro: |
Trigo. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02411naa a2200217 a 4500
001 1840212
005 2016-03-15
008 1995 bl --- 0-- u #d
100 1 $aFEUILLET, C.
245 $aGenetic and physical characterization of the LR1 leaf rust resistance locus in wheat (Triticum aestivum L.).
260 $c1995
520 $aThe objective of this study was to characterize the leaf rust resistance locus Lr1 in wheat. Restriction fragment length polymorphism (RFLP) analysis was performed on the resistant line Lr1/6* Thatcher and the susceptible varieties Thatcher and Frisal, as well as on the segregating F2 populations. Seventeen out of 37 RFLP probes mapping to group 5 chromosomes showed polymorphism between Lr1/6* Thatcher and Frisal, whereas 11 probes were polymorphic between the near-isogenic lines (NILs) Lr1/6* Thatcher and Thatcher. Three of these probes were linked to the resistance gene in the segregating F2 populations. One probe (pTAG621) showed very tight linkage to Lr1 and mapped to a single-copy region on chromosome 5D. The map location of pTAG621 at the end of the long arm of chromosome 5D was confirmed by the absence of the band in the nulli-tetrasomic line N5DT5B of Chinese Spring and a set of deletion lines of Chinese Spring lacking the distal part of 5DL. Twenty-seven breeding lines containing the Lr1 resistance gene in different genetic backgrounds showed the same band as Lr1/6* Thatcher when hybridized with pTAG621. The RFLP marker was converted to a sequence-tagged-site marker using polymerase chain reaction (PCR) amplification. Sequencing of the specific fragment amplified from both NILs revealed point mutations as well as small insertion/deletion events. These were used to design primers that allowed amplification of a specific product only from the resistant line Lr1/6* Thatcher. This STS, specific for the Lr1 resistance gene, will allow efficient selection for the disease resistance gene in wheat breeding programmes. In addition, the identification of a D-genome-specific probe tightly linked to Lr1 should ultimately provide the basis for positional cloning of the gene.
650 $aTrigo
653 $aFerrugem da folha
653 $aGene de resistência
653 $aPCR
653 $aRFLP
700 1 $aMESSMER, M.
700 1 $aSCHACHERMAYR, G.
700 1 $aKELLER, B.
773 $tMolecular and General Genetics$gv. 248, p. 553-562, 1995.
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Registro original: |
Embrapa Trigo (CNPT) |
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Registro Completo
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
24/01/2011 |
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Data da última atualização: |
16/10/2012 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
PIRES, P. P.; WENDLING, I.; AUER, C. G.; KRATZ, D. |
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Afiliação: |
Patrícia Pereira Pires, UFPR; IVAR WENDLING, CNPF; CELSO GARCIA AUER, CNPF; Dagma Kratz, UFPR. |
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Título: |
Diferentes métodos de enxertia em Araucaria angustifolia. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
In: EVENTO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA FLORESTAS, 9., 2010, Colombo. Anais. Colombo: Embrapa Florestas, 2010. 1 CD-ROM. (Embrapa Florestas. Documentos, 198). EVINCI. |
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Descrição Física: |
1 CD-ROM. |
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Idioma: |
Português |
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Thesagro: |
Araucária; Enxerto; Propagação Vegetativa. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/25851/1/144-10.pdf
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Marc: |
LEADER 00624nam a2200181 a 4500
001 1874049
005 2012-10-16
008 2010 bl uuuu u00u1 u #d
100 1 $aPIRES, P. P.
245 $aDiferentes métodos de enxertia em Araucaria angustifolia.
260 $aIn: EVENTO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA FLORESTAS, 9., 2010, Colombo. Anais. Colombo: Embrapa Florestas, 2010. 1 CD-ROM. (Embrapa Florestas. Documentos, 198). EVINCI.$c2010
300 $c1 CD-ROM.
650 $aAraucária
650 $aEnxerto
650 $aPropagação Vegetativa
700 1 $aWENDLING, I.
700 1 $aAUER, C. G.
700 1 $aKRATZ, D.
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Embrapa Florestas (CNPF) |
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