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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
30/01/2017 |
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Data da última atualização: |
02/03/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FERREIRA, R. C. U.; CANÇADO, L. J.; VALLE, C. B. do; CHIARI, L.; SOUZA, A. P. de. |
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Afiliação: |
REBECCA, C. U. FERREIRA, UNICAMP; LETÍCIA J. CANÇADO; CACILDA BORGES DO VALLE, CNPGC; LUCIMARA CHIARI, CNPGC; ANETE P DE SOUZA, UNICAMP. |
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Título: |
Microsatellite loci for Urochloa decumbens (Stapf) R. D. Webster and cross-amplification in other Urochloa species. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
BMC Research Notes, v. 9, n. 152, p. 1-11, 2016. |
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Idioma: |
Português |
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Conteúdo: |
Background: Forage grasses of the African genus Urochloa (syn. Brachiaria) are the basis of Brazilian beef production, and there is a strong demand for high quality, productive and adapted forage plants. Among the approximately 100 species of the genus Urochloa, Urochloa decumbens is one of the most important tropical forage grasses used for pastures due to several of its agronomic attributes. However, the level of understanding of these attributes and the tools with which to control them at the genetic level are limited, mainly due to the apomixis and ploidy level of this species. In this context, the present study aimed to identify and characterize molecular microsatellite markers of U. decumbens and to evaluate their cross-amplification in other Urochloa species. Findings: Microsatellite loci were isolated from a previously constructed enriched library from one U. decumbens genotype. Specific primers were designed for one hundred thirteen loci, and ninety-three primer pairs successfully amplified microsatellite regions, yielding an average of 4.93 alleles per locus. The polymorphism information content ( IC) values of these loci ranged from 0.26 to 0.85 (average 0.68), and the associated discriminating power (DP) values ranged from 0.22 to 0.97 (average 0.77). Cross-amplification studies demonstrated the potential transferability of these microsatellites to four other Urochloa species. Structure analysis revealed the existence of three distinct groups, providing evidence in the allelic pool that U. decumbens is closely related to Urochloa ruziziensis and Urochloa brizantha. The genetic distance values determined using Jaccard?s coefficient ranged from 0.06 to 0.76. Conclusions: The microsatellite markers identified in this study are the first set of molecular markers for U. decumbens species. Their availability will facilitate understanding the genetics of this and other Urochloa species and breeding them, and will be useful for germplasm characterization, linkage mapping and marker-assisted selection. Keywords: Enriched library, Forage, Signalgrass, Simple sequence repeat, Transferability MenosBackground: Forage grasses of the African genus Urochloa (syn. Brachiaria) are the basis of Brazilian beef production, and there is a strong demand for high quality, productive and adapted forage plants. Among the approximately 100 species of the genus Urochloa, Urochloa decumbens is one of the most important tropical forage grasses used for pastures due to several of its agronomic attributes. However, the level of understanding of these attributes and the tools with which to control them at the genetic level are limited, mainly due to the apomixis and ploidy level of this species. In this context, the present study aimed to identify and characterize molecular microsatellite markers of U. decumbens and to evaluate their cross-amplification in other Urochloa species. Findings: Microsatellite loci were isolated from a previously constructed enriched library from one U. decumbens genotype. Specific primers were designed for one hundred thirteen loci, and ninety-three primer pairs successfully amplified microsatellite regions, yielding an average of 4.93 alleles per locus. The polymorphism information content ( IC) values of these loci ranged from 0.26 to 0.85 (average 0.68), and the associated discriminating power (DP) values ranged from 0.22 to 0.97 (average 0.77). Cross-amplification studies demonstrated the potential transferability of these microsatellites to four other Urochloa species. Structure analysis revealed the existence of three distinct groups, providing evidence ... Mostrar Tudo |
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Palavras-Chave: |
Enriched library; Transferability. |
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Thesaurus Nal: |
Forage; Microsatellite repeats; Urochloa brizantha. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/154227/1/Microsatellite-loci-for-Urochloa.pdf
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Marc: |
LEADER 02829naa a2200229 a 4500
001 2062128
005 2017-03-02
008 2016 bl uuuu u00u1 u #d
100 1 $aFERREIRA, R. C. U.
245 $aMicrosatellite loci for Urochloa decumbens (Stapf) R. D. Webster and cross-amplification in other Urochloa species.$h[electronic resource]
260 $c2016
520 $aBackground: Forage grasses of the African genus Urochloa (syn. Brachiaria) are the basis of Brazilian beef production, and there is a strong demand for high quality, productive and adapted forage plants. Among the approximately 100 species of the genus Urochloa, Urochloa decumbens is one of the most important tropical forage grasses used for pastures due to several of its agronomic attributes. However, the level of understanding of these attributes and the tools with which to control them at the genetic level are limited, mainly due to the apomixis and ploidy level of this species. In this context, the present study aimed to identify and characterize molecular microsatellite markers of U. decumbens and to evaluate their cross-amplification in other Urochloa species. Findings: Microsatellite loci were isolated from a previously constructed enriched library from one U. decumbens genotype. Specific primers were designed for one hundred thirteen loci, and ninety-three primer pairs successfully amplified microsatellite regions, yielding an average of 4.93 alleles per locus. The polymorphism information content ( IC) values of these loci ranged from 0.26 to 0.85 (average 0.68), and the associated discriminating power (DP) values ranged from 0.22 to 0.97 (average 0.77). Cross-amplification studies demonstrated the potential transferability of these microsatellites to four other Urochloa species. Structure analysis revealed the existence of three distinct groups, providing evidence in the allelic pool that U. decumbens is closely related to Urochloa ruziziensis and Urochloa brizantha. The genetic distance values determined using Jaccard?s coefficient ranged from 0.06 to 0.76. Conclusions: The microsatellite markers identified in this study are the first set of molecular markers for U. decumbens species. Their availability will facilitate understanding the genetics of this and other Urochloa species and breeding them, and will be useful for germplasm characterization, linkage mapping and marker-assisted selection. Keywords: Enriched library, Forage, Signalgrass, Simple sequence repeat, Transferability
650 $aForage
650 $aMicrosatellite repeats
650 $aUrochloa brizantha
653 $aEnriched library
653 $aTransferability
700 1 $aCANÇADO, L. J.
700 1 $aVALLE, C. B. do
700 1 $aCHIARI, L.
700 1 $aSOUZA, A. P. de
773 $tBMC Research Notes$gv. 9, n. 152, p. 1-11, 2016.
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Registro original: |
Embrapa Gado de Corte (CNPGC) |
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| Registros recuperados : 26 | |
| 2. |  | FERREIRA, R. C. U. (; CHIARI, L.; SOUZA, A. P. Transferibilidade de marcadores microssatélites entre espécies do gênero Urochloa. WORKSHOP MELHORAMENTO VEGETAL, 2., 2016, Campo Grande, MS. Contribuições, Avanços e perspectivas para o Cerrado brasileiro. Anais... Campo Grande, MS: SBMP, 2016.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 11. | | FERREIRA, R. C. U.; LARA, L. A. de C.; CHIARI, L.; BARRIOS, S. C. L.; VALLE, C. B. do; GARCIA, A. A. F.; SOUZA, A. P. de. The first genotyping by sequencing in an interespecific population of Urochloa decumbens. In: CONGRESSO BRASILEIRO DE MELHORAMENTO DE PLANTAS, 9., 2017, Foz de Iguaçu. Melhoramento de plantas: projetando o futuro: resumos das palestras. p. 104| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 12. | | RESENDE, R. M. S.; ASSIS, G. M. L. de; MEDEIROS, S. R. de; MATIDA, E. T.; ARGUELHO, E. G.; FERREIRA, R. C. U. Parâmetros genéticos e melhoramento da leguminosa forrageira Arachis spp. In:SIMPÓSIO INTERNACIONAL SOBRE MELHORAMENTO DE FORRAGEIRAS, 2., 2009, Campo Grande, MS. [Anais]... Campo Grande, MS: Embrapa Gado de Corte, 2009. II SIMF. Comitê editorial: Liana Jank; Lucimara Chiari; Rosângela Maria Simeão Resende. 5 p. 1 CD-ROM. Resumo M06.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 13. | | RESENDE, R. M. S.; ASSIS, G. M. L. de; MEDEIROS, S. R. de; MATIDA, E. T.; ARGUELHO, E. G.; FERREIRA, R. C. U. Parâmetros genéticos e melhoramentos da leguminosa forrageira Arachis spp. In: SIMPÓSIO INTERNACIONAL SOBRE MELHORAMENTO DE FORRAGEIRAS, 2., 2009, Campo Grande, MS. Anais... Campo Grande, MS: Embrapa Gado de Corte, 2009. 4 p. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Acre. |
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| 16. | | FERREIRA, R. C. U.; RESENDE, R. M. S.; ASSIS, G. M. L. de; VALENTIM, J. F.; JANK, L.; VALLE, C. B. do. Seleção de amendoim forrageiro para cultivo em Mato Grosso do Sul. In: JORNADA CIENTÍFICA DA EMBRAPA GADO DE CORTE,5., 2009, Campo Grande, MS. [Anais da ...]. Campo Grande, MS: Embrapa Gado de Corte, 2009. 1 p.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 17. | | DEO, T. G.; FERREIRA, R. C. U.; LARA, L. A. C.; MORAES, A. C. L.; ALVES-PEREIRA, A.; OLIVEIRA, F. A. de; GARCIA, A. A. F.; SANTOS, M. F.; JANK, L.; SOUZA, A. P. de. High-Resolution Linkage Map With Allele Dosage Allows the Identification of Regions Governing Complex Traits and Apospory in Guinea Grass (Megathyrsus maximus). Frontiers in Plant Science, 26 fev.. 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 18. | | RESENDE, R. M. S.; MARTINS, F. B.; VILELA, M. de M.; MORAES, A. C. L.; FERREIRA, R. C. U.; DÉO, T. G.; MOREIRA, F. A.; PASTINA, M. M.; NODA, R. W.; SOUZA, A. P. Genotyping by sequencing approach for autotetraploid Urochloa Ruziziensis genetic breeding. Campinas: Universidade Estadual de Campinas - UNICAMP/Centro de Biologia Molecular e Engenharia Genética - CBMEG, 2019. 1 folder| Tipo: Folder/Folheto/Cartilha |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 19. | | RESENDE, R. M. S.; MARTINS, F. B.; VILELA, M. de M.; MORAES, A. C. L.; FERREIRA, R. C. U.; DÉO, T. G.; PASTINA, M. M.; NODA, R. W.; SOUZA, A. P. Genotyping by sequencing approach for autotetraploidy urochloa. INTERNATIONAL FORAGE OF TURF BREEDING CONFERENCE, 2019, Lake Buena Vista, Fl, USA. A global vision for innovation: abstracts book. University of Florida, 2019. p. 81 IFTBC 2019.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 20. | | MARTINS, F. B.; AONO, A. H.; MORAES, A. da C. L.; FERREIRA, R. C. U.; VILELA, M. de M.; PESSOA FILHO, M. A. C. de P.; RODRIGUES-MOTTA, M.; SIMEÃO, R. M.; SOUZA, A. P. de. Genome-wide family prediction unveils molecular mechanisms underlying the regulation of agronomic traits in Urochloa ruziziensis. Frontiers in Plant Science, v. 14, 2023. Na publicação: Marco Pessoa-Filho.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Cerrados; Embrapa Gado de Corte. |
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| Registros recuperados : 26 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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