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Registro Completo |
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
01/04/2016 |
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Data da última atualização: |
29/12/2017 |
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Autoria: |
PAZZINI, J. M.; DE NARDI, A. B.; HUPPES, R. R.; GERING, A. P.; FERREIRA, M. G. P. A.; SILVEIRA, C. P. B.; LUZZI, M. C.; SANTOS, R. |
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Afiliação: |
JOSIANE M. PAZZINI, FCAV/UNESP; ANRIGO B. De NARDI, FCAV/UNESP; RAFAEL R. HUPPES, FCAV/UNESP; ANA P. GERING, FCAV/UNESP; MARÍLIA G. P. A. FERREIRA, FCAV/UNESP; CAMILA P. B. SILVEIRA, FCAV/UNESP; MAYARA C. LUZZI, FCAV/UNESP; ROMEU SANTOS, FCAV/UNESP. |
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Título: |
Method to abtain platelet-rich plasma from rabbits (Oryctolagus cuniculus). |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 36, n. 1, p. 39-44, jan. 2016. |
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Idioma: |
Inglês |
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Conteúdo: |
cific time and gravitational forces. Thus, the present work aimed to study a method of double centrifugation to obtain PRP in order to evaluate the effective increase of platelet concentration in the final product, the preparation of PRP gel, and to optimize preparation time of the final sample. Fifteen female White New Zealand rabbits underwent blood sampling for the preparation of PRP. Samples were separated in two sterile tubes containing sodium citrate. Tubes were submitted to the double centrifugation protocol, with lid closed and 1600 revolutions per minute (rpm) for 10 minutes, resulting in the separation of red blood cells, plasma with platelets and leucocytes. After were opened and plasma was pipetted and transferred into another sterile tube. Plasma was centrifuged again at 000rpm for 10 minutes; as a result it was split into two parts: on the top, consisting of platelet-poor plasma (PPP) and at the bottom of the platelet button. Part of the PPP was discarded so that only 1ml remained in the tube along with the platelet button. This material was gently agitated to promote platelets resuspension and activated when added 0.3ml of calcium gluconate, resulting in PRP gel. Double centrifugation protocol was able to make platelet concentration 3 times higher in relation to the initial blood sample. The volume of calcium gluconate used for platelet activation was 0.3ml, and was sufficient to coagulate the sample. Coagulation time ranged from 8 to 20 minutes, with an average of 17.6 minutes. Therefore, time of blood centrifugation until to obtain PRP gel took only 40 minutes. It was concluded that PRP was successfully obtained by double centrifugation protocol, which is able to increase the platelet concentration in the sample compared with whole blood, allowing its use in surgical procedures. Furthermore, the preparation time is appropriate to obtain PRP in just 40 minutes, and calcium gluconate is able to promote the activation of platelets. Menoscific time and gravitational forces. Thus, the present work aimed to study a method of double centrifugation to obtain PRP in order to evaluate the effective increase of platelet concentration in the final product, the preparation of PRP gel, and to optimize preparation time of the final sample. Fifteen female White New Zealand rabbits underwent blood sampling for the preparation of PRP. Samples were separated in two sterile tubes containing sodium citrate. Tubes were submitted to the double centrifugation protocol, with lid closed and 1600 revolutions per minute (rpm) for 10 minutes, resulting in the separation of red blood cells, plasma with platelets and leucocytes. After were opened and plasma was pipetted and transferred into another sterile tube. Plasma was centrifuged again at 000rpm for 10 minutes; as a result it was split into two parts: on the top, consisting of platelet-poor plasma (PPP) and at the bottom of the platelet button. Part of the PPP was discarded so that only 1ml remained in the tube along with the platelet button. This material was gently agitated to promote platelets resuspension and activated when added 0.3ml of calcium gluconate, resulting in PRP gel. Double centrifugation protocol was able to make platelet concentration 3 times higher in relation to the initial blood sample. The volume of calcium gluconate used for platelet activation was 0.3ml, and was sufficient to coagulate the sample. Coagulation time ranged from 8 to 20 minutes, with an avera... Mostrar Tudo |
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Palavras-Chave: |
Espécies em extinção; Platelet concentration; Pratelet-rich plasma; Preservação; Revascularization. |
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Thesagro: |
Banco de germoplasma; Biotecnologia; Criopreservação. |
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Thesaurus Nal: |
Rabbits. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/141905/1/Method-to-obtain.pdf
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Marc: |
LEADER 02888naa a2200313 a 4500
001 2042339
005 2017-12-29
008 2016 bl uuuu u00u1 u #d
100 1 $aPAZZINI, J. M.
245 $aMethod to abtain platelet-rich plasma from rabbits (Oryctolagus cuniculus).
260 $c2016
520 $acific time and gravitational forces. Thus, the present work aimed to study a method of double centrifugation to obtain PRP in order to evaluate the effective increase of platelet concentration in the final product, the preparation of PRP gel, and to optimize preparation time of the final sample. Fifteen female White New Zealand rabbits underwent blood sampling for the preparation of PRP. Samples were separated in two sterile tubes containing sodium citrate. Tubes were submitted to the double centrifugation protocol, with lid closed and 1600 revolutions per minute (rpm) for 10 minutes, resulting in the separation of red blood cells, plasma with platelets and leucocytes. After were opened and plasma was pipetted and transferred into another sterile tube. Plasma was centrifuged again at 000rpm for 10 minutes; as a result it was split into two parts: on the top, consisting of platelet-poor plasma (PPP) and at the bottom of the platelet button. Part of the PPP was discarded so that only 1ml remained in the tube along with the platelet button. This material was gently agitated to promote platelets resuspension and activated when added 0.3ml of calcium gluconate, resulting in PRP gel. Double centrifugation protocol was able to make platelet concentration 3 times higher in relation to the initial blood sample. The volume of calcium gluconate used for platelet activation was 0.3ml, and was sufficient to coagulate the sample. Coagulation time ranged from 8 to 20 minutes, with an average of 17.6 minutes. Therefore, time of blood centrifugation until to obtain PRP gel took only 40 minutes. It was concluded that PRP was successfully obtained by double centrifugation protocol, which is able to increase the platelet concentration in the sample compared with whole blood, allowing its use in surgical procedures. Furthermore, the preparation time is appropriate to obtain PRP in just 40 minutes, and calcium gluconate is able to promote the activation of platelets.
650 $aRabbits
650 $aBanco de germoplasma
650 $aBiotecnologia
650 $aCriopreservação
653 $aEspécies em extinção
653 $aPlatelet concentration
653 $aPratelet-rich plasma
653 $aPreservação
653 $aRevascularization
700 1 $aDE NARDI, A. B.
700 1 $aHUPPES, R. R.
700 1 $aGERING, A. P.
700 1 $aFERREIRA, M. G. P. A.
700 1 $aSILVEIRA, C. P. B.
700 1 $aLUZZI, M. C.
700 1 $aSANTOS, R.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 36, n. 1, p. 39-44, jan. 2016.
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Registro original: |
Embrapa Unidades Centrais (AI-SEDE) |
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| Registros recuperados : 19 | |
| 5. |  | FERREIRA, M. G. R.; CUTRIM, M. B. de A.; MUNIZ, Y. S.; FONSECA, I. M. Massa seca da parte aérea da cultura do arroz cultivada em latossolo tratado com granulado bioclástico marinho. In: ENCONTRO REGIONAL DE CIÊNCIA DO SOLO NA AMAZÔNIA ORIENTAL, 3., 2018, São Luís, MA. Anais... São Luís, MA: EDUFMA, 2018. p. 79.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Cocais. |
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| 6. | | FERREIRA, M. G. R.; MUNIZ, Y. S.; CUTRIM, M. B. de A.; FONSECA, I. M. Produtividade de grãos de arroz cultivado em latossolo tratado com Lithothamnium. In: ENCONTRO REGIONAL DE CIÊNCIA DO SOLO NA AMAZÔNIA ORIENTAL, 3., 2018, São Luís, MA. Anais... São Luís, MA: EDUFMA, 2018. p. 80.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Cocais. |
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| 7. | | MELO-STERZA, F. A.; ANDRADE, E. R.; FERREIRA, M. G. C. R.; NOGUEIRA, E.; CARDOSO, C. J. T. Anatomofisiologia do ciclo estral de fêmeas bovinas. In: NOGUEIRA, E.; SILVA, J. C. B.; NICACIO, A. C.; MINGOTI, G. Z. (Ed.). Ultrassonografia na reprodução e avaliação de carcaças em bovinos. Brasília, DF: Embrapa, 2017. p. 15-36.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Pantanal. |
|  |
| 8. | | MELO-STERZA, F. A.; ANDRADE, E. R.; FERREIRA, M. G. C. R.; NOGUEIRA, E.; CARDOSO, C. J. T. Anatomofisiologia do ciclo estral de fêmeas bovinas. In: NOGUEIRA, E.; SILVA, J. C. B.; NICACIO, A. C.; MINGOTI, G. Z. (Ed.). Ultrassonografia na reprodução e avaliação de carcaças em bovinos. Brasília, DF: Embrapa, 2021. Cap. 1. E-book.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Pantanal. |
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| 10. | | CARVALHO, J. O. M.; BARROSO, G. R. P.; SANTOS, M. R. A.; FERREIRA, M. G. R.; RODRIGUES, C. D. S. Teor de macronutrientes e produção de biomassa de adubos verdes em Rondônia, Brasil. Revista Brasileira de Agroecologia, v. 2, n. 2, p. 1099-1102, 2007. Resumos do V CBA - Manejo de Agrossistemas Sustentáveis.| Tipo: Artigo em Anais de Congresso / Nota Técnica | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Rondônia. |
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| 11. | | PAZZINI, J. M.; DE NARDI, A. B.; HUPPES, R. R.; GERING, A. P.; FERREIRA, M. G. P. A.; SILVEIRA, C. P. B.; LUZZI, M. C.; SANTOS, R. Method to abtain platelet-rich plasma from rabbits (Oryctolagus cuniculus). Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 36, n. 1, p. 39-44, jan. 2016.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 12. | | PAZZINI, J. M.; DE NARDI, A. B.; HUPPES, R. R.; GERING, A. P.; FERREIRA, M. G. P. A.; SILVEIRA, C. P. B.; LUZZI, M. C.; OLIVEIRA, J. A. Utilização de plasma rico em plaquetas para estimulação da angiogênese em flape de padrão axial toracodorsal em coelhos. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 36, n. 2, p. 108-118, fev. 2016.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 13. | | PAULA, A. L. de; GIAROLA, N. F. B.; FOGAÇA, A. M.; DENCK, G.; FERREIRA, M. G.; PEIXOTO, R. T. dos G.; MAIA, C. M. B. de F.; KAZMIERCZAK, R. A adição de biocarvão altera a distribuição de partículas de um Latossolo vermelho-amarelo argiloso? In: REUNIÃO PARANAENSE DE CIÊNCIA DO SOLO, 6., 2019, Ponta Grossa. O desafio da produção agropecuária com baixo impacto ambiental: anais... Ponta Grossa: UEPG/PROEX, 2019. p. 841-844.| Tipo: Artigo em Anais de Congresso |
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| 14. | | FERREIRA, M. G. C. R.; SOUZA, M. B.; SPRÍCIGO, J. F. W.; JONATHAN VINIVICUS DOS SANTOS, J. V. dos; POEHLAND, R.; DODE, M. A. N.; STERZA, F. de A. M. Características da trimetilação da lisina 4 da histona 3 em oócitos bovinos submetidos a maturação in vitro e criopreservação: resultados preliminares. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 29., 2015, Gramado. Anais... [S.l.]: SBTE, 2015. p. 423.| Tipo: Resumo em Anais de Congresso |
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| 15. | | SOUZA, A. C. F.; PASCOLI, A. L.; FERREIRA, M. G. P. A.; REIS FILHO, N. P.; SILVA, I. C. R.; SANTOS, R. R.; FARO, A. M.; DE NARDI, A. B. Mastocitoma cutâneo canino: estudo retrospectivo dos casos atendidos pelo Serviço de Oncologia do Hospital Veterinário da FCAV-Unesp, Campus Jaboticabal, de 2005 a 20151. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 38, n. 9, p. 1808-1817, setembro 2018 Título em inglês: Canine cutaneous mast cell tumors: retrospective study of cases attended by the oncology service at the Veterinary Hospital of FCAV-Unesp, Jaboticabal, from 2005 to 2015.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 16. | | CARDOSO, C. J. T.; SILVA, W. A. L. da; KISCHEL, H.; ARRUDA, E. D. S.; FERREIRA, M. G. C. R.; LIMA, A. C. B. de; OLIVEIRA, M. V. M.; SOUZA, M. B.; NOGUEIRA, E.; STERZA, F. A. M. Parameters blood and viability oocyte Girolando's breed supplemented with linseed (Linum usitatissimum L.) - preliminary results. Animal Reproduction, v.13, n.3, p.524, Jul./Sept. 2016 Proceedings of the 30th Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Foz do Iguaçu, PR, Brazil, August 25th to 27th, 2016, and 32nd Meeting of the European Embryo Transfer Association (AETE); Barcelona, Spain,...| Tipo: Resumo em Anais de Congresso |
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| 17. | | SILVA, K. C. da; ANACHE, N. A.; CARDOSO, C. J. T.; BEZERRA, A. de O.; JARA, J. do P.; NOGUEIRA, E.; FERREIRA, M. G. C. R.; STERZA, F. de A. M.; RODRIGUES, W. B.; SARAIVA, N. Z. Suplementação de búfalas com gordura protegida: efeitos na produção leiteira e taxa de prenhez à IATF. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 32., 2018, Florianópolis. Anais... Jaboticabal: Sociedade Brasileira de Tecnologia de Embriões, 2018. p. 43-44.| Tipo: Resumo em Anais de Congresso |
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| 18. | | CARDOSO, C. J. T.; SILVA, K. C. da; ANACHE, N. A.; RODRIGUES, W. B.; LIMA, A. C. B. de; FERREIRA, M. G. C. R.; STERZA, F. de A. M.; NOGUEIRA, E. Supplementation with protected FAT in the dairy buffalo cows pregnancy rate. Animal Reproduction, v.14, n. 3, p. 719, jul./sept. 2017. Proceedings of the 31st Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Cabo de Santo Agostinho, PE, Brazil, August 17th to 19th, 2017. Abstracts.| Tipo: Resumo em Anais de Congresso |
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| 19. | | SILVA, W. A. L da; POEHLAND, R.; OLIVEIRA, C. C. de; FERREIRA, M. G. C. R.; ALMEIDA, R. G. de; CÁCERES, M. B. S.; MACEDO, G. G.; COSTA e SILVA, E. V. da; ALVES, F. V.; NOGUEIRA, E.; MELO-STERZA, F. de A. Shading effect on physiological parameters and in vitro embryoproduction of tropical adapted Nellore heifers in integratedcrop-livestock-forest systems. Tropical Animal Health and Production, v. 52, n. 5, p. 2273-2281, 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Pantanal. |
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| Registros recuperados : 19 | |
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