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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
24/08/2011 |
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Data da última atualização: |
25/07/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
KATIKI, L. M.; FERREIRA, J. F. S.; ZAJAC, A. M.; MASLER, C.; LINDSAY, D. S.; CHAGAS, A. C. de S.; AMARANTE, A. F. T. |
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Afiliação: |
LUCIANA M. KATIKI, ISNTITUTO DE ZOOTECNIA/NOVA ODESSA; JORGE F. S. FERREIRA, Appalachian Farming Systems Research Center (USDA-ARS); ANNE M. ZAJAC, VIRGINIA-MARYLAND REGIONAL COLLEGE OF VETERINARY MEDICINE, VIRGINIA TECH, BLACKSBURG; CAROL MASLER, NEMATOLOGY LABORATORY, USDA-ARS, BARC/ BELTSVILLE; DAVID S. LINDSAY, VIRGINIA-MARYLAND REGIONAL COLLEGE OF VETERINARY MEDICINE, VIRGINIA TECH/ BLACKSBURG; ANA CAROLINA DE SOUZA CHAGAS, CPPSE; ALESSANDRO F. T. AMARANTE, UNESP/BOTUCATU. |
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Título: |
Caenorhabditis elegans as a model to screen plant extracts and compounds as natural anthelmintics for veterinary use. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Veterinary Parasitology, v. 182, n. 2-4, p. 264-268, dec. 2011. |
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DOI: |
https://doi.org/10.1016/j.vetpar.2011.05.020 |
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Idioma: |
Inglês |
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Conteúdo: |
The most challenging obstacles to testing products for their anthelmintic activity are: (1) establishing a suitable nematode in vitro assay that can evaluate potential product use against a parasitic nematode of interest and (2) preparation of extracts that can be redissolved in solvents that are miscible in the test medium and are at concentrations well tolerated by the nematode system used for screening. The use of parasitic nematodes as a screening system is hindered by the difficulty of keeping them alive for long periods outside their host and by the need to keep infected animals as sources of eggs or adults when needed. This method uses the free-living soil nematode Caenorhabditis elegans as a system to screen products for their potential anthelmintic effect against small ruminant gastrointestinal nematodes, including Haemonchus contortus. This modified method uses only liquid axenic medium, instead of agar plates inoculated with Escherichia coli, and two selective sieves to obtain adult nematodes. During screening, the use of either balanced salt solution (M-9) or distilled water resulted in averages of 99.7 (±0.73)% and 96.36 (±2.37)% motile adults, respectively. Adult worms tolerated DMSO, ethanol, methanol, and Tween 80 at 1% and 2%, while Labrasol® (a bioenhancer with low toxicity to mammals) and Tween 20 were toxic to C. elegans at 1% and were avoided as solvents. The high availability, ease of culture, and rapid proliferation of C. elegans make it a useful screening system to test plant extracts and other phytochemical compounds to investigate their potential anthelmintic activity against parasitic nematodes. MenosThe most challenging obstacles to testing products for their anthelmintic activity are: (1) establishing a suitable nematode in vitro assay that can evaluate potential product use against a parasitic nematode of interest and (2) preparation of extracts that can be redissolved in solvents that are miscible in the test medium and are at concentrations well tolerated by the nematode system used for screening. The use of parasitic nematodes as a screening system is hindered by the difficulty of keeping them alive for long periods outside their host and by the need to keep infected animals as sources of eggs or adults when needed. This method uses the free-living soil nematode Caenorhabditis elegans as a system to screen products for their potential anthelmintic effect against small ruminant gastrointestinal nematodes, including Haemonchus contortus. This modified method uses only liquid axenic medium, instead of agar plates inoculated with Escherichia coli, and two selective sieves to obtain adult nematodes. During screening, the use of either balanced salt solution (M-9) or distilled water resulted in averages of 99.7 (±0.73)% and 96.36 (±2.37)% motile adults, respectively. Adult worms tolerated DMSO, ethanol, methanol, and Tween 80 at 1% and 2%, while Labrasol® (a bioenhancer with low toxicity to mammals) and Tween 20 were toxic to C. elegans at 1% and were avoided as solvents. The high availability, ease of culture, and rapid proliferation of C. elegans make it a useful scree... Mostrar Tudo |
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Palavras-Chave: |
Anthelmintic plants; C elegans. |
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Thesaurus Nal: |
gastrointestinal nematodes; plant extracts. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/40411/1/PROCI-2011.00093.pdf
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Marc: |
LEADER 02474naa a2200253 a 4500
001 1898592
005 2022-07-25
008 2011 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.vetpar.2011.05.020$2DOI
100 1 $aKATIKI, L. M.
245 $aCaenorhabditis elegans as a model to screen plant extracts and compounds as natural anthelmintics for veterinary use.$h[electronic resource]
260 $c2011
520 $aThe most challenging obstacles to testing products for their anthelmintic activity are: (1) establishing a suitable nematode in vitro assay that can evaluate potential product use against a parasitic nematode of interest and (2) preparation of extracts that can be redissolved in solvents that are miscible in the test medium and are at concentrations well tolerated by the nematode system used for screening. The use of parasitic nematodes as a screening system is hindered by the difficulty of keeping them alive for long periods outside their host and by the need to keep infected animals as sources of eggs or adults when needed. This method uses the free-living soil nematode Caenorhabditis elegans as a system to screen products for their potential anthelmintic effect against small ruminant gastrointestinal nematodes, including Haemonchus contortus. This modified method uses only liquid axenic medium, instead of agar plates inoculated with Escherichia coli, and two selective sieves to obtain adult nematodes. During screening, the use of either balanced salt solution (M-9) or distilled water resulted in averages of 99.7 (±0.73)% and 96.36 (±2.37)% motile adults, respectively. Adult worms tolerated DMSO, ethanol, methanol, and Tween 80 at 1% and 2%, while Labrasol® (a bioenhancer with low toxicity to mammals) and Tween 20 were toxic to C. elegans at 1% and were avoided as solvents. The high availability, ease of culture, and rapid proliferation of C. elegans make it a useful screening system to test plant extracts and other phytochemical compounds to investigate their potential anthelmintic activity against parasitic nematodes.
650 $agastrointestinal nematodes
650 $aplant extracts
653 $aAnthelmintic plants
653 $aC elegans
700 1 $aFERREIRA, J. F. S.
700 1 $aZAJAC, A. M.
700 1 $aMASLER, C.
700 1 $aLINDSAY, D. S.
700 1 $aCHAGAS, A. C. de S.
700 1 $aAMARANTE, A. F. T.
773 $tVeterinary Parasitology$gv. 182, n. 2-4, p. 264-268, dec. 2011.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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Registro Completo
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
17/10/2022 |
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Data da última atualização: |
17/10/2022 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
MARTINS, L.; MARTINHÃO, L.; GARCIA, I.; RIBAS, D.; GRÁZIA, J. G.; FIGUEIREDO, R. A.; VIANA, J. H. M. |
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Afiliação: |
L. MARTINS, Universidade de Brasília; L. MARTINHÃO, Universidade de Brasília; I. GARCIA, Universidade do Estado de Mato Grosso; D. RIBAS, Universidade do Estado de Mato Grosso; J. G. GRÁZIA, Apoyar Biotech; RICARDO ALAMINO FIGUEIREDO, Cenargen; JOAO HENRIQUE MOREIRA VIANA, Cenargen. |
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Título: |
Use of human recombinant FSH for in vitro embryo production in cattle. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
In: INTERNATIONAL CONGRESS ON ANIMAL REPRODUCTION, 19., 2022, Bologna, Italy. Abstract Book... Bologna: ICAR, 2022. |
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Idioma: |
Inglês |
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Notas: |
Poster M106. Na publicação: R. Figueiredo. |
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Palavras-Chave: |
Catte; FSH; RhFSH. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1147345/1/Resumo-M106-ICAR-2022.pdf
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Marc: |
LEADER 00697nam a2200217 a 4500
001 2147345
005 2022-10-17
008 2022 bl uuuu u00u1 u #d
100 1 $aMARTINS, L.
245 $aUse of human recombinant FSH for in vitro embryo production in cattle.$h[electronic resource]
260 $aIn: INTERNATIONAL CONGRESS ON ANIMAL REPRODUCTION, 19., 2022, Bologna, Italy. Abstract Book... Bologna: ICAR$c2022
500 $aPoster M106. Na publicação: R. Figueiredo.
653 $aCatte
653 $aFSH
653 $aRhFSH
700 1 $aMARTINHÃO, L.
700 1 $aGARCIA, I.
700 1 $aRIBAS, D.
700 1 $aGRÁZIA, J. G.
700 1 $aFIGUEIREDO, R. A.
700 1 $aVIANA, J. H. M.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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