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Registro Completo |
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
26/04/2010 |
Data da última atualização: |
15/02/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
VIREQUE, A. A.; CAMARGO, L. S. de A.; SERAPIÃO, R. V.; ROSA E SILVA, A. A. M.; WATANABE, Y. F.; FERREIRA, E. M.; NAVARRO, P. A. A. S.; MARTINS, W. P.; FERRIANI, R. A. |
Afiliação: |
A. A. VIREQUE, USP; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; RAQUEL VARELLA SERAPIÃO, UFRRJ; A. A. M. ROSA E SILVA, Universidade de Brasília; Y. G. WATANABE, Vitrogen; E. M. FERREIRA, USP; P. A. A. S. NAVARRO, USP; W. P. MARTINS, USP; R. A. FERRIANI, USP. |
Título: |
Preimplantation development and expression of Hsp-70 and Bax genes in bovine blastocysts derived from oocytes matured in alpha-MEM supplemented with growth factors and synthetic macromolecules. |
Ano de publicação: |
2009 |
Fonte/Imprenta: |
Theriogenology, v. 71, n. 4, p. 620-627, 2009. |
DOI: |
https://doi.org/10.1016/j.theriogenology.2008.09.028 |
Idioma: |
Inglês |
Conteúdo: |
In vitro culture conditions affect both the maternal and embryonic expression of genes and is likely to alter both oocyte and embryo developmental competence. The search for better and less variable culture conditions simulating those in vivo has led to the development of defined culture media, with lower impact on the molecular reprogramming of oocytes and embryos. We evaluated embryo development and relative abundance (RA) of Hsp-70 and Bax transcripts in bovine blastocysts produced from oocytes matured in a chemically defined IVM system with synthetic polymers. Immature cumulus oocyte complexes (COCs) were matured for 22-24h in alpha-MEM supplemented with IGF-1, insulin, 0.1% polyvinyl alcohol (PVA), or 0.1% polyvinylpyrrolidone (PVP), but without FSH or LH. The control group consisted of COCs matured in TCM plus FSH and 10% estrous cow serum. After fertilization, presumptive zygotes were co-cultured with cumulus cells until 224 h post-insemination. Total RNA was isolated from embryo pools, reverse transcribed into cDNA, and subjected to transcript analysis by real-time PCR. Cleavage rate was higher (P<0.05) for the control group (68.3%) than for the PVA (54.4%) and PVP-40 (58.3%) groups. Nevertheless, there was no difference among the PVA, PVP-40 and control groups in blastocyst or hatching rates. Similarly, no difference in relative abundance of Hsp-70 and Bax transcripts was detected in comparison to the control group. We inferred that bovine oocytes can be matured in serum- and gonadotrophin-free medium supplemented with PVA or PVP, enriched with IGF-I and insulin, without altering post-cleavage development and relative abundance of some genes associated with stress and apoptosis. MenosIn vitro culture conditions affect both the maternal and embryonic expression of genes and is likely to alter both oocyte and embryo developmental competence. The search for better and less variable culture conditions simulating those in vivo has led to the development of defined culture media, with lower impact on the molecular reprogramming of oocytes and embryos. We evaluated embryo development and relative abundance (RA) of Hsp-70 and Bax transcripts in bovine blastocysts produced from oocytes matured in a chemically defined IVM system with synthetic polymers. Immature cumulus oocyte complexes (COCs) were matured for 22-24h in alpha-MEM supplemented with IGF-1, insulin, 0.1% polyvinyl alcohol (PVA), or 0.1% polyvinylpyrrolidone (PVP), but without FSH or LH. The control group consisted of COCs matured in TCM plus FSH and 10% estrous cow serum. After fertilization, presumptive zygotes were co-cultured with cumulus cells until 224 h post-insemination. Total RNA was isolated from embryo pools, reverse transcribed into cDNA, and subjected to transcript analysis by real-time PCR. Cleavage rate was higher (P<0.05) for the control group (68.3%) than for the PVA (54.4%) and PVP-40 (58.3%) groups. Nevertheless, there was no difference among the PVA, PVP-40 and control groups in blastocyst or hatching rates. Similarly, no difference in relative abundance of Hsp-70 and Bax transcripts was detected in comparison to the control group. We inferred that bovine oocytes can be matured in ... Mostrar Tudo |
Palavras-Chave: |
Bovine oocytes; In vitro maturation. |
Thesaurus Nal: |
embryogenesis. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/711876/1/Preimplantation-development-and-expression-of-Hsp-70-and-Bax-genes-in-bovine-blastocysts.pdf
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Marc: |
LEADER 02626naa a2200265 a 4500 001 1711876 005 2024-02-15 008 2009 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1016/j.theriogenology.2008.09.028$2DOI 100 1 $aVIREQUE, A. A. 245 $aPreimplantation development and expression of Hsp-70 and Bax genes in bovine blastocysts derived from oocytes matured in alpha-MEM supplemented with growth factors and synthetic macromolecules. 260 $c2009 520 $aIn vitro culture conditions affect both the maternal and embryonic expression of genes and is likely to alter both oocyte and embryo developmental competence. The search for better and less variable culture conditions simulating those in vivo has led to the development of defined culture media, with lower impact on the molecular reprogramming of oocytes and embryos. We evaluated embryo development and relative abundance (RA) of Hsp-70 and Bax transcripts in bovine blastocysts produced from oocytes matured in a chemically defined IVM system with synthetic polymers. Immature cumulus oocyte complexes (COCs) were matured for 22-24h in alpha-MEM supplemented with IGF-1, insulin, 0.1% polyvinyl alcohol (PVA), or 0.1% polyvinylpyrrolidone (PVP), but without FSH or LH. The control group consisted of COCs matured in TCM plus FSH and 10% estrous cow serum. After fertilization, presumptive zygotes were co-cultured with cumulus cells until 224 h post-insemination. Total RNA was isolated from embryo pools, reverse transcribed into cDNA, and subjected to transcript analysis by real-time PCR. Cleavage rate was higher (P<0.05) for the control group (68.3%) than for the PVA (54.4%) and PVP-40 (58.3%) groups. Nevertheless, there was no difference among the PVA, PVP-40 and control groups in blastocyst or hatching rates. Similarly, no difference in relative abundance of Hsp-70 and Bax transcripts was detected in comparison to the control group. We inferred that bovine oocytes can be matured in serum- and gonadotrophin-free medium supplemented with PVA or PVP, enriched with IGF-I and insulin, without altering post-cleavage development and relative abundance of some genes associated with stress and apoptosis. 650 $aembryogenesis 653 $aBovine oocytes 653 $aIn vitro maturation 700 1 $aCAMARGO, L. S. de A. 700 1 $aSERAPIÃO, R. V. 700 1 $aROSA E SILVA, A. A. M. 700 1 $aWATANABE, Y. F. 700 1 $aFERREIRA, E. M. 700 1 $aNAVARRO, P. A. A. S. 700 1 $aMARTINS, W. P. 700 1 $aFERRIANI, R. A. 773 $tTheriogenology$gv. 71, n. 4, p. 620-627, 2009.
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Embrapa Gado de Leite (CNPGL) |
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Biblioteca(s): |
Embrapa Semiárido. |
Data corrente: |
05/07/1996 |
Data da última atualização: |
01/02/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
Nacional - B |
Autoria: |
MORAES, G. J. de; FLECHTMANN, C. H. W. |
Afiliação: |
GILBERTO JOSÉ DE MORAES, CPATSA; CARLOS H. W. FLECHTMANN. |
Título: |
Paralelo entre dois complexos Euphorbiaceae Tetranychidae (Acari) no Nordeste e Sudeste do Brasil. |
Ano de publicação: |
1980 |
Fonte/Imprenta: |
Anais da Escola Superior de Agricultura Luiz de Queiroz, v. 37, n. 2, p. 743-745, 1980. |
Idioma: |
Português |
Conteúdo: |
Relata-se o paralelo existente entre a ocorrencia do completo Jatropha gossypifolia L.- Tetranychus bastoli Tulle, Baker & Sales no Nordeste e do complexo Ricinus communis L. Tetranychus desertorum Banko/T. ludeni Zacher no Sudeste em areas depredadas pelo homem. |
Palavras-Chave: |
Insect; Nordeste; Northeast; Southeast; Sudeste; Tetranychus baslosi; Tetranychus desortorum. |
Thesagro: |
Ácaro; Entomologia; Inseto; Ricinus Communis. |
Thesaurus NAL: |
entomology; mites; natural resources. |
Categoria do assunto: |
O Insetos e Entomologia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/158811/1/Gilberto-1980.pdf
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Marc: |
LEADER 01101naa a2200301 a 4500 001 1132612 005 2023-02-01 008 1980 bl uuuu u00u1 u #d 100 1 $aMORAES, G. J. de 245 $aParalelo entre dois complexos Euphorbiaceae Tetranychidae (Acari) no Nordeste e Sudeste do Brasil. 260 $c1980 520 $aRelata-se o paralelo existente entre a ocorrencia do completo Jatropha gossypifolia L.- Tetranychus bastoli Tulle, Baker & Sales no Nordeste e do complexo Ricinus communis L. Tetranychus desertorum Banko/T. ludeni Zacher no Sudeste em areas depredadas pelo homem. 650 $aentomology 650 $amites 650 $anatural resources 650 $aÁcaro 650 $aEntomologia 650 $aInseto 650 $aRicinus Communis 653 $aInsect 653 $aNordeste 653 $aNortheast 653 $aSoutheast 653 $aSudeste 653 $aTetranychus baslosi 653 $aTetranychus desortorum 700 1 $aFLECHTMANN, C. H. W. 773 $tAnais da Escola Superior de Agricultura Luiz de Queiroz$gv. 37, n. 2, p. 743-745, 1980.
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