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| Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
Registro Completo |
Biblioteca(s): |
Embrapa Pecuária Sudeste. |
Data corrente: |
11/11/2010 |
Data da última atualização: |
30/06/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
CHIARATTI, M. R.; FERREIRA, C. R.; MEIRELLES, F. V.; MÉO, S. C.; PERECIN, F.; SMITH, L. C.; FERRAZ, M. L.; SÁ FILHO, M. F. de; GIMENES, L. U.; BARUSELLI, P. S.; GASPARRINI, B.; GARCIA, J. M. |
Afiliação: |
MARCOS ROBERTO CHIARATTI, FZEA-USP, Pirassununga-SP; CHRISTINA RAMIRES FERREIRA, FCAV-UNESP/JABOTICABAL-SP; FLÁVIO VIEIRA MEIRELLES, FZEA-USP/PIRASSUNUNGA-SP; SIMONE CRISTINA MÉO, CPPSE; FELIPE PERECIN, FCAV-UNESP/JABOTICABAL-SP; LAWRENCE CHARLES SMITH, CRRA, UNIVERSITE DE MONTREAL, SAINT-HYACINTHE-QC, CANADA.; MÁRCIO LEÃO FERRAZ, VIDA REPRODUTIVA CONSULTORIA, CRAVINHOS-SP, BRAZIL; MANOEL FRANCISCO DE SÁ FILHO, REPRODUÇÃO ANIMAL, UNIVERSIDADE DE SÃO PAULO/ SÃO PAULO-SP; LINDSAY UNNO GIMENES, USP/SÃO PAULO, SP; PIETRO SAMPAIO BARUSELLI, REPRODUÇÃO ANIMAL, UNIVERSIDADE DE SÃO PAULO/ SÃO PAULO-SP; BIANCA GASPARRINI, DISCIZIA, FEDERICO II UNIVERSITY, NAPOLI, ITALY.; JOAQUIM MANSANO GARCIA, FCAV-UNESP/JABOTICABAL-SP. |
Título: |
Xenooplasmic transfer between buffalo and bovine enables development of homoplasmic offspring. |
Ano de publicação: |
2010 |
Fonte/Imprenta: |
Cellular Reprogramming, v. 12, n. 2, 2010. |
DOI: |
10.1089/cell1009.0076. |
Idioma: |
Português |
Conteúdo: |
Nuclear-mitochondrial incompatibilities may be responsible for the development failure reported in embryos and fetuses produced by interspecies somatic cell nuclear transfer (iSCNT). Herein we performed xenooplasmic transfer (XOT) by introducing 10 to 15% of buffalo ooplasm into bovine zygotes to assess its effect on the persistence of buffalo mitochondrial DNA (mtDNA). Blastocyst rates were not compromised by XOT in comparison to both in vitro fertilized embryos and embryos produced by transfer of bovine ooplasm into bovine zygotes. Moreover, offspring were born after transfer of XOT embryos to recipient cows. Buffalo mtDNA introduced in zygotes was still present at the blastocyst stage (8.3 vs. 9.3%, p¼0.11), indicating unaltered heteroplasmy during early development. Nonetheless, no vestige of buffalo mtDNA was found in offspring, indicating a drift to homoplasmy during later stages of development. In conclusion, we show that the buffalo mtDNA introduced by XOT into a bovine zygote do not compromise embryo development. On the other hand, buffalo mtDNA was not inherited by offspring indicating a possible failure in the process of interspecies mtDNA replication. |
Palavras-Chave: |
Buffalo; Oocyte cytoplasm. |
Categoria do assunto: |
G Melhoramento Genético |
Marc: |
LEADER 02010naa a2200289 a 4500 001 1866805 005 2023-06-30 008 2010 bl uuuu u00u1 u #d 024 7 $a10.1089/cell1009.0076.$2DOI 100 1 $aCHIARATTI, M. R. 245 $aXenooplasmic transfer between buffalo and bovine enables development of homoplasmic offspring.$h[electronic resource] 260 $c2010 520 $aNuclear-mitochondrial incompatibilities may be responsible for the development failure reported in embryos and fetuses produced by interspecies somatic cell nuclear transfer (iSCNT). Herein we performed xenooplasmic transfer (XOT) by introducing 10 to 15% of buffalo ooplasm into bovine zygotes to assess its effect on the persistence of buffalo mitochondrial DNA (mtDNA). Blastocyst rates were not compromised by XOT in comparison to both in vitro fertilized embryos and embryos produced by transfer of bovine ooplasm into bovine zygotes. Moreover, offspring were born after transfer of XOT embryos to recipient cows. Buffalo mtDNA introduced in zygotes was still present at the blastocyst stage (8.3 vs. 9.3%, p¼0.11), indicating unaltered heteroplasmy during early development. Nonetheless, no vestige of buffalo mtDNA was found in offspring, indicating a drift to homoplasmy during later stages of development. In conclusion, we show that the buffalo mtDNA introduced by XOT into a bovine zygote do not compromise embryo development. On the other hand, buffalo mtDNA was not inherited by offspring indicating a possible failure in the process of interspecies mtDNA replication. 653 $aBuffalo 653 $aOocyte cytoplasm 700 1 $aFERREIRA, C. R. 700 1 $aMEIRELLES, F. V. 700 1 $aMÉO, S. C. 700 1 $aPERECIN, F. 700 1 $aSMITH, L. C. 700 1 $aFERRAZ, M. L. 700 1 $aSÁ FILHO, M. F. de 700 1 $aGIMENES, L. U. 700 1 $aBARUSELLI, P. S. 700 1 $aGASPARRINI, B. 700 1 $aGARCIA, J. M. 773 $tCellular Reprogramming$gv. 12, n. 2, 2010.
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Embrapa Pecuária Sudeste (CPPSE) |
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Registro Completo
Biblioteca(s): |
Embrapa Agroindústria de Alimentos. |
Data corrente: |
31/12/2022 |
Data da última atualização: |
12/12/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
SANTOS, T. B. DOS; FREIRE NETO, R. DA S.; COLLANTES, N. F.; CHÁVEZ, D. W. H.; QUEIROZ, V. A. V.; CARVALHO, C. W. P. de. |
Afiliação: |
THAÍS BARBOSA DOS SANTOS, UFRRJ; RAIMUNDO DA SILVA FREIRE NETO, UFRRJ; NATHALIA FERREIRA COLLANTES, UFRRJ; DAVY WILLIAM HIDALGO CHÁVEZ, UFRRJ; VALERIA APARECIDA VIEIRA QUEIROZ, CNPMS; CARLOS WANDERLEI PILER DE CARVALHO, CTAA. |
Título: |
Exploring starches from varied sorghum genotypes compared to commercial maize starch. |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
Journal of Food Process Engineering, v. 46, n. 10, 2023, |
DOI: |
https://doi.org/10.1111/jfpe.14251 |
Idioma: |
Inglês |
Conteúdo: |
Sorghum has been lately attracted the attention for human consumption as a glutenfree cereal rich in bioactive compounds. Sorghum starches isolated from brown (BRS305), red (BRS308), and white (CMSXS180) genotypes were compared to commercial maize starch, based on proximate composition, particle size distribution, microstructure, X-ray diffraction, amylose and total starch content, solubility index, swelling power, pasting, and thermal properties. The carbohydrate content of sorghum starch samples ranged from 98.24 to 99.23 g/100 g (dry basis). The particle size distribution of the CMSXS180 sorghum genotype was very similar to commercial maize starch. The relative crystallinity values varied from 29.83% to 30.39%. The water solubility index and swelling power of sorghum starches were lower than those of maize starch. Sorghum genotypes paste profiles were similar, but greatly differed from maize showing the highest final viscosity (4042.0?4444.5 cP) compared to maize starch (3767.5 cP) and lower gelatinization enthalpy (?H = 9?10.5 J/g) than maize starch (11.8 J/g). The results showed some distinct properties of sorghum starches when compared to maize starch, which may contribute to provide alternative uses, particularly in food products requiring very high viscosity and retrogradation. |
Palavras-Chave: |
Isolation; Pearson's correlation. |
Thesagro: |
Amido; Genótipo; Milho; Sorgo; Viscosidade. |
Thesaurus NAL: |
Food technology; Pastes; Swelling (materials); Viscosity. |
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
Marc: |
LEADER 02232naa a2200325 a 4500 001 2150506 005 2023-12-12 008 2023 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1111/jfpe.14251$2DOI 100 1 $aSANTOS, T. B. DOS 245 $aExploring starches from varied sorghum genotypes compared to commercial maize starch.$h[electronic resource] 260 $c2023 520 $aSorghum has been lately attracted the attention for human consumption as a glutenfree cereal rich in bioactive compounds. Sorghum starches isolated from brown (BRS305), red (BRS308), and white (CMSXS180) genotypes were compared to commercial maize starch, based on proximate composition, particle size distribution, microstructure, X-ray diffraction, amylose and total starch content, solubility index, swelling power, pasting, and thermal properties. The carbohydrate content of sorghum starch samples ranged from 98.24 to 99.23 g/100 g (dry basis). The particle size distribution of the CMSXS180 sorghum genotype was very similar to commercial maize starch. The relative crystallinity values varied from 29.83% to 30.39%. The water solubility index and swelling power of sorghum starches were lower than those of maize starch. Sorghum genotypes paste profiles were similar, but greatly differed from maize showing the highest final viscosity (4042.0?4444.5 cP) compared to maize starch (3767.5 cP) and lower gelatinization enthalpy (?H = 9?10.5 J/g) than maize starch (11.8 J/g). The results showed some distinct properties of sorghum starches when compared to maize starch, which may contribute to provide alternative uses, particularly in food products requiring very high viscosity and retrogradation. 650 $aFood technology 650 $aPastes 650 $aSwelling (materials) 650 $aViscosity 650 $aAmido 650 $aGenótipo 650 $aMilho 650 $aSorgo 650 $aViscosidade 653 $aIsolation 653 $aPearson's correlation 700 1 $aFREIRE NETO, R. DA S. 700 1 $aCOLLANTES, N. F. 700 1 $aCHÁVEZ, D. W. H. 700 1 $aQUEIROZ, V. A. V. 700 1 $aCARVALHO, C. W. P. de 773 $tJournal of Food Process Engineering$gv. 46, n. 10, 2023
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