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Registro Completo |
Biblioteca(s): |
Embrapa Agricultura Digital; Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
21/11/2012 |
Data da última atualização: |
08/01/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
MARRACCINI, P.; VINECKY, F.; ALVES, G. S. C.; RAMOS, H. J. O.; ELBELT, S.; VIEIRA, N. G.; CARNEIRO, F. A.; SUJII, P. S.; ALEKCEVETCH, J. C.; SILVA, V. A.; DaMATTA, F. M.; FERRÃO, M. A. G.; LEROY, T.; POT, D.; VIEIRA, L. G. E.; SILVA, F. R. da; ANDRADE, A. C. |
Afiliação: |
PIERRE MARRACCINI, Cenargen, CIRAD; FELIPE VINECKY, Cenargen; GABRIEL S. C. ALVES, Cenargen; HUMBERTO J. O. RAMOS, IAPAR; SONIA ELBELT, Cenargen, CIRAD; NATALIA G. VIEIRA, Cenargen; FERNANDA A. CARNEIRO, Cenargen; PATRICIA S. SUJII, Cenargen; JEAN C. ALEKCEVETCH, Cenargen; VÂNIA A. SILVA, UFV; FÁBIO M. DAMATTA, UFV; MARIA AMELIA GAVA FERRÃO, SAPC; THIERRY LEROY, CIRAD; DAVID POT, CIRAD; LUIZ G. E. VIEIRA, SAPC; FELIPE RODRIGUES DA SILVA, CNPTIA; ALAN CARVALHO ANDRADE, CENARGEN. |
Título: |
Differentially expressed genes and proteins upon drought acclimation in tolerant and sensitive genotypes of Coffea canephora. |
Ano de publicação: |
2012 |
Fonte/Imprenta: |
Journal of Experimental Botany, Oxford, v. 63, n. 11, p. 4191-4212, 2012. |
DOI: |
10.1093/jxb/ers103 |
Idioma: |
Inglês |
Conteúdo: |
The aim of this study was to investigate the molecular mechanisms underlying drought acclimation in coffee plants by the identification of candidate genes (CGs) using different approaches. The first approach used the data generated during the Brazilian Coffee expressed sequence tag (EST) project to select 13 CGs by an in silico analysis (electronic northern). The second approach was based on screening macroarrays spotted with plasmid DNA (coffee ESTs) with separate hybridizations using leaf cDNA probes from drought-tolerant and susceptible clones of Coffea canephora var. Conilon, grown under different water regimes. This allowed the isolation of seven additional CGs. The third approach used two-dimensional gel electrophoresis to identify proteins displaying differential accumulation in leaves of drought-tolerant and susceptible clones of C. canephora. Six of them were characterized by MALDI-TOF-MS/MS (matrix-assisted laser desorption-time of flight-tandem mass spectrometry) and the corresponding proteins were identified. Finally, additional CGs were selected from the literature, and quantitative real-time polymerase chain reaction (qPCR) was performed to analyse the expression of all identified CGs. Altogether, >40 genes presenting differential gene expression during drought acclimation were identified, some of them showing different expression profiles between drought-tolerant and susceptible clones. Based on the obtained results, it can be concluded that factors involved a complex network of responses probably involving the abscisic signalling pathway and nitric oxide are major molecular determinants that might explain the better efficiency in controlling stomata closure and transpiration displayed by drought-tolerant clones of C. canephora. MenosThe aim of this study was to investigate the molecular mechanisms underlying drought acclimation in coffee plants by the identification of candidate genes (CGs) using different approaches. The first approach used the data generated during the Brazilian Coffee expressed sequence tag (EST) project to select 13 CGs by an in silico analysis (electronic northern). The second approach was based on screening macroarrays spotted with plasmid DNA (coffee ESTs) with separate hybridizations using leaf cDNA probes from drought-tolerant and susceptible clones of Coffea canephora var. Conilon, grown under different water regimes. This allowed the isolation of seven additional CGs. The third approach used two-dimensional gel electrophoresis to identify proteins displaying differential accumulation in leaves of drought-tolerant and susceptible clones of C. canephora. Six of them were characterized by MALDI-TOF-MS/MS (matrix-assisted laser desorption-time of flight-tandem mass spectrometry) and the corresponding proteins were identified. Finally, additional CGs were selected from the literature, and quantitative real-time polymerase chain reaction (qPCR) was performed to analyse the expression of all identified CGs. Altogether, >40 genes presenting differential gene expression during drought acclimation were identified, some of them showing different expression profiles between drought-tolerant and susceptible clones. Based on the obtained results, it can be concluded that factors involved a... Mostrar Tudo |
Palavras-Chave: |
Candidate gene; Differential expression; Drought acclimation; Proteomic; Proteômica; Real time PCR. |
Thesagro: |
Café; Coffea Canephora; Genética. |
Thesaurus Nal: |
Genetics. |
Categoria do assunto: |
-- |
Marc: |
LEADER 03043naa a2200445 a 4500 001 1940133 005 2020-01-08 008 2012 bl uuuu u00u1 u #d 024 7 $a10.1093/jxb/ers103$2DOI 100 1 $aMARRACCINI, P. 245 $aDifferentially expressed genes and proteins upon drought acclimation in tolerant and sensitive genotypes of Coffea canephora.$h[electronic resource] 260 $c2012 520 $aThe aim of this study was to investigate the molecular mechanisms underlying drought acclimation in coffee plants by the identification of candidate genes (CGs) using different approaches. The first approach used the data generated during the Brazilian Coffee expressed sequence tag (EST) project to select 13 CGs by an in silico analysis (electronic northern). The second approach was based on screening macroarrays spotted with plasmid DNA (coffee ESTs) with separate hybridizations using leaf cDNA probes from drought-tolerant and susceptible clones of Coffea canephora var. Conilon, grown under different water regimes. This allowed the isolation of seven additional CGs. The third approach used two-dimensional gel electrophoresis to identify proteins displaying differential accumulation in leaves of drought-tolerant and susceptible clones of C. canephora. Six of them were characterized by MALDI-TOF-MS/MS (matrix-assisted laser desorption-time of flight-tandem mass spectrometry) and the corresponding proteins were identified. Finally, additional CGs were selected from the literature, and quantitative real-time polymerase chain reaction (qPCR) was performed to analyse the expression of all identified CGs. Altogether, >40 genes presenting differential gene expression during drought acclimation were identified, some of them showing different expression profiles between drought-tolerant and susceptible clones. Based on the obtained results, it can be concluded that factors involved a complex network of responses probably involving the abscisic signalling pathway and nitric oxide are major molecular determinants that might explain the better efficiency in controlling stomata closure and transpiration displayed by drought-tolerant clones of C. canephora. 650 $aGenetics 650 $aCafé 650 $aCoffea Canephora 650 $aGenética 653 $aCandidate gene 653 $aDifferential expression 653 $aDrought acclimation 653 $aProteomic 653 $aProteômica 653 $aReal time PCR 700 1 $aVINECKY, F. 700 1 $aALVES, G. S. C. 700 1 $aRAMOS, H. J. O. 700 1 $aELBELT, S. 700 1 $aVIEIRA, N. G. 700 1 $aCARNEIRO, F. A. 700 1 $aSUJII, P. S. 700 1 $aALEKCEVETCH, J. C. 700 1 $aSILVA, V. A. 700 1 $aDaMATTA, F. M. 700 1 $aFERRÃO, M. A. G. 700 1 $aLEROY, T. 700 1 $aPOT, D. 700 1 $aVIEIRA, L. G. E. 700 1 $aSILVA, F. R. da 700 1 $aANDRADE, A. C. 773 $tJournal of Experimental Botany, Oxford$gv. 63, n. 11, p. 4191-4212, 2012.
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Embrapa Agricultura Digital (CNPTIA) |
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Biblioteca(s): |
Embrapa Amazônia Oriental. |
Data corrente: |
14/01/2005 |
Data da última atualização: |
21/06/2022 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
MIRANDA, I. L.; CARVALHO, J. O. P. de. |
Afiliação: |
JOÃO OLEGÁRIO PEREIRA DE CARVALHO, CPATU. |
Título: |
Silvicultura de cinco espécies arbóreas de interesse econômico nativas da Amazônia. |
Ano de publicação: |
1998 |
Fonte/Imprenta: |
In: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA (AVALIAÇÃO 1997-1998), 8., 1998, Belém, PA. Resumos. Belém, PA: FCAP-Unidade de Apoio à Pesquisa e Pós-Graduação, 1998. |
Páginas: |
p. 183. |
Idioma: |
Português |
Palavras-Chave: |
Ipê-amarelo. |
Thesagro: |
Bagassa Guianensis; Comportamento de Variedade; Copaíba; Copaifera Multijuga; Cumaru; Dipteryx Odorata; Floresta; Germinação; Hymenaea Courbaril; Jatobá; Tabebuia Serratifolia; Tatajuba. |
Categoria do assunto: |
K Ciência Florestal e Produtos de Origem Vegetal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/406105/1/Silvicultura-de-cinco-especies-arboreas.pdf
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Marc: |
LEADER 00904nam a2200277 a 4500 001 1406105 005 2022-06-21 008 1998 bl uuuu u00u1 u #d 100 1 $aMIRANDA, I. L. 245 $aSilvicultura de cinco espécies arbóreas de interesse econômico nativas da Amazônia. 260 $aIn: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA (AVALIAÇÃO 1997-1998), 8., 1998, Belém, PA. Resumos. Belém, PA: FCAP-Unidade de Apoio à Pesquisa e Pós-Graduação$c1998 300 $ap. 183. 650 $aBagassa Guianensis 650 $aComportamento de Variedade 650 $aCopaíba 650 $aCopaifera Multijuga 650 $aCumaru 650 $aDipteryx Odorata 650 $aFloresta 650 $aGerminação 650 $aHymenaea Courbaril 650 $aJatobá 650 $aTabebuia Serratifolia 650 $aTatajuba 653 $aIpê-amarelo 700 1 $aCARVALHO, J. O. P. de
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