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| 4. |  | CAPALBO, D. M. F.; BONILLA, M. H.; ALBERTAZZI, F.; FERNANDEZ-NORTHCOTE, E. N.; GONZALEZ, C.; ESCOBAR, F. J.; RODRIGUEZ, J.; ORJUELA, M. A. The Latin America Multi - Country capacity building for compliance with the Cartagena Protocol on Biosafety Project: Brazil - Colombia - Costa Rita - Perú - (Lac Biosafety Project). In: MEETING OF THE CONFERENCE OF THE PARTIES SERVING AS THE MEETING OF THE PARTIES TO THE CARTEGENA PROTOCOL ON BIOSAFETY, 5., 2010, Aichi-Nagoya. Abstract of Posters for COP-MOP5. Pôster L05.| Biblioteca(s): Embrapa Meio Ambiente. |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
06/09/2016 |
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Data da última atualização: |
13/11/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
TREML, D.; VENTURELLI, G. L.; BROD, F. C. A.; FARIA, J. C.; ARISI, A. C. M. |
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Afiliação: |
DIANA TREML, UNIVERSIDADE FEDERAL DE SANTA CATARINA; GUSTAVO L. VENTURELLI, UNIVERSIDADE FEDERAL DE SANTA CATARINA; FABIO C. A. BROD, UNIVERSIDADE FEDERAL DE SANTA CATARINA; JOSIAS CORREA DE FARIA, CNPAF; ANA C. M. ARISI, UNIVERSIDADE FEDERAL DE SANTA CATARINA. |
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Título: |
Development of an event-specific hydrolysis probe quantitative real-time polymerase chain reaction assay for Embrapa 5.1 genetically modified common bean (Phaseolus vulgaris). |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Journal of Agricultural and Food Chemistry, Easton, v. 62, n. 49, p. 11994-12000, Dec. 2014. |
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DOI: |
10.1021/jf503928m |
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Idioma: |
Inglês |
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Conteúdo: |
A genetically modified (GM) common bean event, namely Embrapa 5.1, resistant to the bean golden mosaic virus (BGMV), was approved for commercialization in Brazil. Brazilian regulation for genetically modified organism (GMO) labeling requires that any food containing more than 1% GMO be labeled. The event-specific polymerase chain reaction (PCR) method has been the primary trend for GMO identification and quantitation because of its high specificity based on the flanking sequence. This work reports the development of an event-specific assay, named FGM, for Embrapa 5.1 detection and quantitation by use of SYBR Green or hydrolysis probe. The FGM assay specificity was tested for Embrapa 2.3 event (a noncommercial GM common bean also resistant to BGMV), 46 non-GM common bean varieties, and other crop species including maize, GM maize, soybean, and GM soybean. The FGM assay showed high specificity to detect the Embrapa 5.1 event. Standard curves for the FGM assay presented a mean efficiency of 95% and a limit of detection (LOD) of 100 genome copies in the presence of background DNA. The primers and probe developed are suitable for the detection and quantitation of Embrapa 5.1. |
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Palavras-Chave: |
GMO; QPCR; SYBR green; TaqMan. |
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Thesagro: |
Biotecnologia; Engenharia genética; Feijão; Organismo transgênico; Phaseolus vulgaris. |
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Thesaurus NAL: |
Beans. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02142naa a2200301 a 4500
001 2052475
005 2017-11-13
008 2014 bl uuuu u00u1 u #d
024 7 $a10.1021/jf503928m$2DOI
100 1 $aTREML, D.
245 $aDevelopment of an event-specific hydrolysis probe quantitative real-time polymerase chain reaction assay for Embrapa 5.1 genetically modified common bean (Phaseolus vulgaris).$h[electronic resource]
260 $c2014
520 $aA genetically modified (GM) common bean event, namely Embrapa 5.1, resistant to the bean golden mosaic virus (BGMV), was approved for commercialization in Brazil. Brazilian regulation for genetically modified organism (GMO) labeling requires that any food containing more than 1% GMO be labeled. The event-specific polymerase chain reaction (PCR) method has been the primary trend for GMO identification and quantitation because of its high specificity based on the flanking sequence. This work reports the development of an event-specific assay, named FGM, for Embrapa 5.1 detection and quantitation by use of SYBR Green or hydrolysis probe. The FGM assay specificity was tested for Embrapa 2.3 event (a noncommercial GM common bean also resistant to BGMV), 46 non-GM common bean varieties, and other crop species including maize, GM maize, soybean, and GM soybean. The FGM assay showed high specificity to detect the Embrapa 5.1 event. Standard curves for the FGM assay presented a mean efficiency of 95% and a limit of detection (LOD) of 100 genome copies in the presence of background DNA. The primers and probe developed are suitable for the detection and quantitation of Embrapa 5.1.
650 $aBeans
650 $aBiotecnologia
650 $aEngenharia genética
650 $aFeijão
650 $aOrganismo transgênico
650 $aPhaseolus vulgaris
653 $aGMO
653 $aQPCR
653 $aSYBR green
653 $aTaqMan
700 1 $aVENTURELLI, G. L.
700 1 $aBROD, F. C. A.
700 1 $aFARIA, J. C.
700 1 $aARISI, A. C. M.
773 $tJournal of Agricultural and Food Chemistry, Easton$gv. 62, n. 49, p. 11994-12000, Dec. 2014.
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Embrapa Arroz e Feijão (CNPAF) |
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