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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
04/05/1992 |
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Data da última atualização: |
04/05/1992 |
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Autoria: |
FERNANDEZ-LOPEZ, J. A.; ALMELA, L.; SOLEDAD ALMANSA, M.; LOPEZ-ROCA, J. M. |
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Afiliação: |
Universidad de Murcia, Facultad de Ciencias Quimicas y Matematicas, Campus de Espinardo 30100-Murcia, Spain. |
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Título: |
Partial purification and properties of chlorophyllase from chlorotic Citrus limon leaves. |
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Ano de publicação: |
1992 |
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Fonte/Imprenta: |
Phytochemistry, v.31, n.2, p.447-449, 1992. |
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ISSN: |
0031-9422 |
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Idioma: |
Inglês |
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Conteúdo: |
Chlorophyllase from Citrus limon grown under iron deficiency was enriched 86-fold after solubilization with SDS, using (NH4)2SO4 precipitation and gel filtration Sephadex G-100. The optimum pH of the enzyme showed a specific activity of 12.9 nkat mg - 1 protein at pH 7.8 and 27 graus in the present of 35% acetone and 12 mM citrate. The enzyme behaved as a protein of Mr 158 000 on Sephacryl S-200 gel filtration. |
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Palavras-Chave: |
Fitoquimica. |
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Thesagro: |
Clorose. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00954naa a2200193 a 4500
001 1633235
005 1992-05-04
008 1992 bl uuuu u00u1 u #d
022 $a0031-9422
100 1 $aFERNANDEZ-LOPEZ, J. A.
245 $aPartial purification and properties of chlorophyllase from chlorotic Citrus limon leaves.
260 $c1992
520 $aChlorophyllase from Citrus limon grown under iron deficiency was enriched 86-fold after solubilization with SDS, using (NH4)2SO4 precipitation and gel filtration Sephadex G-100. The optimum pH of the enzyme showed a specific activity of 12.9 nkat mg - 1 protein at pH 7.8 and 27 graus in the present of 35% acetone and 12 mM citrate. The enzyme behaved as a protein of Mr 158 000 on Sephacryl S-200 gel filtration.
650 $aClorose
653 $aFitoquimica
700 1 $aALMELA, L.
700 1 $aSOLEDAD ALMANSA, M.
700 1 $aLOPEZ-ROCA, J. M.
773 $tPhytochemistry$gv.31, n.2, p.447-449, 1992.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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