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Registro Completo |
Biblioteca(s): |
Embrapa Clima Temperado. |
Data corrente: |
04/08/2023 |
Data da última atualização: |
04/08/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
PEREIRA, R. S.; COSTA, V. V.; GOMES, G. L. M.; CAMPANA, P. R. V.; PÁDUA, R. M. de; BARBOSA, M.; OKI, Y.; HEIDEN, G.; FERNANDES, G. W.; OLIVEIRA, D. M. de; SOUZA, D. G.; TEIXEIRA, M. M.; BRAGA, F. C. |
Afiliação: |
ROSÂNGELA SANTOS PEREIRA, Universidade Federal de Minas Gerais; VIVIAN VASCONCELOS COSTA, Universidade Federal de Minas Gerais; GABRIEL LUIZ MENEZES GOMES, Universidade Federal de Minas Gerais; PRISCILLA RODRIGUES VALADARES CAMPANA, Universidade Federal de Minas Gerais; RODRIGO MAIA DE PÁDUA, Universidade Federal de Minas Gerais; MILTON BARBOSA, Universidade Federal de Minas Gerais; YUMI OKI, Universidade Federal de Minas Gerais; GUSTAVO HEIDEN, CPACT; GERALDO WILSON FERNANDES, Universidade Federal de Minas Gerais; DJALMA MENEZES DE OLIVEIRA, Universidade Estadual do Sudoeste da Bahia; DANIELE G. SOUZA, Universidade Federal de Minas Gerais; MAURO MARTINS TEIXEIRA, Universidade Federal de Minas Gerais; FERNÃO CASTRO BRAGA, Universidade Federal de Minas Gerais. |
Título: |
Anti-Zika Virus Activity of Plant Extracts Containing Polyphenols and Triterpenes on Vero CCL-81 and Human Neuroblastoma SH-SY5Y Cells. |
Ano de publicação: |
2022 |
Fonte/Imprenta: |
Chemistry & Biodiversity, v. 19, n. 4, e202100842, April 2022. |
DOI: |
https://doi.org/10.1002/cbdv.202100842 |
Idioma: |
Inglês |
Conteúdo: |
Zika virus (ZIKV) infection is a global threat associated to neurological disorders in adults and microcephaly in children born to infected mothers. No vaccine or drug is available against ZIKV. We herein report the anti-ZIKV activity of 36 plant extracts containing polyphenols and/or triterpenes. ZIKV-infected Vero CCL-81 cells were treated with samples at non-cytotoxic concentrations, determined by MTT and LDH assays. One third of the extracts elicited concentration-dependent anti-ZIKV effect, with viral loads reduction from 0.4 to 3.8?log units. The 12 active extracts were tested on ZIKV-infected SH-SY5Y cells and significant reductions of viral loads (in log units) were induced by Maytenus ilicifolia (4.5?log), Terminalia phaeocarpa (3.7?log), Maytenus rigida (1.7?log) and Echinodorus grandiflorus (1.7?log) extracts. Median cytotoxic concentration (CC50) of these extracts in Vero cells were higher than in SH-SY5Y lineage. M. ilicifolia (IC50=16.8±10.3??g/mL, SI=3.4) and T. phaeocarpa (IC50=22.0±6.8??g/mL, SI=4.8) were the most active extracts. UPLC-ESI-MS/MS analysis of M. ilicifolia extract led to the identification of 7 triterpenes, of which lupeol and a mixture of friedelin/friedelinol showed no activity against ZIKV. The composition of T. phaeocarpa extract comprises phenolic acids, ellagitannins and flavonoids, as recently reported by us. In conclusion, the anti-ZIKV activity of 12 plant extracts is here described for the first time and polyphenols and triterpenes were identified as the probable bioactive constituents of T. phaeocarpa and M. ilicifolia, respectively. MenosZika virus (ZIKV) infection is a global threat associated to neurological disorders in adults and microcephaly in children born to infected mothers. No vaccine or drug is available against ZIKV. We herein report the anti-ZIKV activity of 36 plant extracts containing polyphenols and/or triterpenes. ZIKV-infected Vero CCL-81 cells were treated with samples at non-cytotoxic concentrations, determined by MTT and LDH assays. One third of the extracts elicited concentration-dependent anti-ZIKV effect, with viral loads reduction from 0.4 to 3.8?log units. The 12 active extracts were tested on ZIKV-infected SH-SY5Y cells and significant reductions of viral loads (in log units) were induced by Maytenus ilicifolia (4.5?log), Terminalia phaeocarpa (3.7?log), Maytenus rigida (1.7?log) and Echinodorus grandiflorus (1.7?log) extracts. Median cytotoxic concentration (CC50) of these extracts in Vero cells were higher than in SH-SY5Y lineage. M. ilicifolia (IC50=16.8±10.3??g/mL, SI=3.4) and T. phaeocarpa (IC50=22.0±6.8??g/mL, SI=4.8) were the most active extracts. UPLC-ESI-MS/MS analysis of M. ilicifolia extract led to the identification of 7 triterpenes, of which lupeol and a mixture of friedelin/friedelinol showed no activity against ZIKV. The composition of T. phaeocarpa extract comprises phenolic acids, ellagitannins and flavonoids, as recently reported by us. In conclusion, the anti-ZIKV activity of 12 plant extracts is here described for the first time and polyphenols and triterpenes w... Mostrar Tudo |
Thesagro: |
Biodiversidade; Extrato Vegetal; Vírus. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1155672/1/Artigo-Anti8208Zika-Virus-Activity-of-Plant-Extracts.pdf
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Marc: |
LEADER 02560naa a2200313 a 4500 001 2155672 005 2023-08-04 008 2022 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1002/cbdv.202100842$2DOI 100 1 $aPEREIRA, R. S. 245 $aAnti-Zika Virus Activity of Plant Extracts Containing Polyphenols and Triterpenes on Vero CCL-81 and Human Neuroblastoma SH-SY5Y Cells.$h[electronic resource] 260 $c2022 520 $aZika virus (ZIKV) infection is a global threat associated to neurological disorders in adults and microcephaly in children born to infected mothers. No vaccine or drug is available against ZIKV. We herein report the anti-ZIKV activity of 36 plant extracts containing polyphenols and/or triterpenes. ZIKV-infected Vero CCL-81 cells were treated with samples at non-cytotoxic concentrations, determined by MTT and LDH assays. One third of the extracts elicited concentration-dependent anti-ZIKV effect, with viral loads reduction from 0.4 to 3.8?log units. The 12 active extracts were tested on ZIKV-infected SH-SY5Y cells and significant reductions of viral loads (in log units) were induced by Maytenus ilicifolia (4.5?log), Terminalia phaeocarpa (3.7?log), Maytenus rigida (1.7?log) and Echinodorus grandiflorus (1.7?log) extracts. Median cytotoxic concentration (CC50) of these extracts in Vero cells were higher than in SH-SY5Y lineage. M. ilicifolia (IC50=16.8±10.3??g/mL, SI=3.4) and T. phaeocarpa (IC50=22.0±6.8??g/mL, SI=4.8) were the most active extracts. UPLC-ESI-MS/MS analysis of M. ilicifolia extract led to the identification of 7 triterpenes, of which lupeol and a mixture of friedelin/friedelinol showed no activity against ZIKV. The composition of T. phaeocarpa extract comprises phenolic acids, ellagitannins and flavonoids, as recently reported by us. In conclusion, the anti-ZIKV activity of 12 plant extracts is here described for the first time and polyphenols and triterpenes were identified as the probable bioactive constituents of T. phaeocarpa and M. ilicifolia, respectively. 650 $aBiodiversidade 650 $aExtrato Vegetal 650 $aVírus 700 1 $aCOSTA, V. V. 700 1 $aGOMES, G. L. M. 700 1 $aCAMPANA, P. R. V. 700 1 $aPÁDUA, R. M. de 700 1 $aBARBOSA, M. 700 1 $aOKI, Y. 700 1 $aHEIDEN, G. 700 1 $aFERNANDES, G. W. 700 1 $aOLIVEIRA, D. M. de 700 1 $aSOUZA, D. G. 700 1 $aTEIXEIRA, M. M. 700 1 $aBRAGA, F. C. 773 $tChemistry & Biodiversity$gv. 19, n. 4, e202100842, April 2022.
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Registro original: |
Embrapa Clima Temperado (CPACT) |
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Registro Completo
Biblioteca(s): |
Embrapa Gado de Corte. |
Data corrente: |
22/07/2020 |
Data da última atualização: |
01/09/2020 |
Tipo da produção científica: |
Comunicado Técnico/Recomendações Técnicas |
Autoria: |
PEREIRA, M. de A.; COSTA, F. P.; MONTAGNER, D. B.; EUCLIDES, V. P. B.; ARAUJO, A. R. de; BARBOSA, R. A.; SOUZA, J. A. B. A. |
Afiliação: |
MARIANA DE ARAGAO PEREIRA, CNPGC; Fernando Paim Costa, CNPGC - pesquisador aposentado; DENISE BAPTAGLIN MONTAGNER, CNPGC; VALERIA PACHECO BATISTA EUCLIDES, CNPGC; ALEXANDRE ROMEIRO DE ARAUJO, CNPGC; RODRIGO AMORIM BARBOSA, CNPGC; Joaquim Antonio Basilio Angelo Souza, Acadêmico de Zootecnia, UEMS, Bolsista PIBIC do CNPq. |
Título: |
Pastagens: condicionantes econômicos e seus efeitos nas decisões de formação e manejo. |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
Campo Grande, MS: Embrapa Gado de Corte, 2020. |
Série: |
(Embrapa Gado de Corte. Comunicado Técnico, 150) |
Idioma: |
Português |
Conteúdo: |
Nesta publicação, aspectos teóricos e práticos são abordados de forma ampla, permitindo ao leitor não apenas entender as questões econômicas associadas às pastagens, mas, sobretudo, de pô-las em prática. Uma série de métodos e técnicas são apresentados, oferecendo diversas alternativas de análise para atender as necessidades de produtores com perfil e nível gerencial diferenciados. |
Palavras-Chave: |
Carne bovina; Custos; Degradação; Falha na semeadura; Forrageira; Manejo do pastejo. |
Thesagro: |
Pastagem. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/215118/1/COT-150-FInal-em-Alta.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/214726/1/COT-150-FInal-em-Alta.pdf
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Marc: |
LEADER 01230nam a2200277 a 4500 001 2124196 005 2020-09-01 008 2020 bl uuuu u0uu1 u #d 100 1 $aPEREIRA, M. de A. 245 $aPastagens$bcondicionantes econômicos e seus efeitos nas decisões de formação e manejo.$h[electronic resource] 260 $aCampo Grande, MS: Embrapa Gado de Corte$c2020 490 $a(Embrapa Gado de Corte. Comunicado Técnico, 150) 520 $aNesta publicação, aspectos teóricos e práticos são abordados de forma ampla, permitindo ao leitor não apenas entender as questões econômicas associadas às pastagens, mas, sobretudo, de pô-las em prática. Uma série de métodos e técnicas são apresentados, oferecendo diversas alternativas de análise para atender as necessidades de produtores com perfil e nível gerencial diferenciados. 650 $aPastagem 653 $aCarne bovina 653 $aCustos 653 $aDegradação 653 $aFalha na semeadura 653 $aForrageira 653 $aManejo do pastejo 700 1 $aCOSTA, F. P. 700 1 $aMONTAGNER, D. B. 700 1 $aEUCLIDES, V. P. B. 700 1 $aARAUJO, A. R. de 700 1 $aBARBOSA, R. A. 700 1 $aSOUZA, J. A. B. A.
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