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Registro Completo |
Biblioteca(s): |
Embrapa Agrobiologia; Embrapa Agroindústria de Alimentos; Embrapa Agropecuária Oeste; Embrapa Algodão; Embrapa Amapá; Embrapa Amazônia Ocidental; Embrapa Amazônia Oriental; Embrapa Arroz e Feijão; Embrapa Caprinos e Ovinos; Embrapa Cerrados; Embrapa Cocais; Embrapa Florestas; Embrapa Gado de Corte; Embrapa Gado de Leite; Embrapa Hortaliças; Embrapa Instrumentação; Embrapa Meio Norte / UEP-Parnaíba; Embrapa Meio-Norte; Embrapa Pantanal; Embrapa Pecuária Sul; Embrapa Rondônia; Embrapa Roraima; Embrapa Semiárido; Embrapa Soja; Embrapa Solos; Embrapa Suínos e Aves; Embrapa Unidades Centrais; Embrapa Uva e Vinho. MenosEmbrapa Agrobiologia; Embrapa Agroindústria de Alimentos; Embrapa Agropecuária Oeste; Embrapa Algodão; Embrapa Amapá; Embrapa Amazônia Ocidental; Embrapa Amazônia Oriental; Embrapa Arroz e Feijão; Embrapa Caprinos e Ovinos; Embrapa Cerrados... Mostrar Todas |
Data corrente: |
31/01/2007 |
Data da última atualização: |
24/04/2013 |
Tipo da produção científica: |
Documentos |
Autoria: |
FERNANDES, E. N.; ROMEIRO, A. R.; ASSIS, C. R. de. |
Título: |
Políticas ambientais aplicadas à questão do aquecimento global. |
Ano de publicação: |
2006 |
Fonte/Imprenta: |
Juiz de Fora: Embrapa Gado de Leite, 2006. |
Páginas: |
48 p. |
Série: |
(Embrapa Gado de Leite. Documentos, 115). |
Idioma: |
Português |
Conteúdo: |
O problema ambiental: aquecimento global; As controvérsias; Dificuldades de tratamento das questões ambientais globais; Soluções propostas; As políticas ambientais. |
Palavras-Chave: |
Aquecimento global; Aquecimento golobal; Economia ecológica; Instrumento econômico; Instrumentos econômicos; Questão ambiental. |
Thesagro: |
Agricultura; Economia; Educação Ambiental; Meio Ambiente; Política Ambiental. |
Categoria do assunto: |
-- P Recursos Naturais, Ciências Ambientais e da Terra |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/81988/1/Politica-ambientais-aplicada.pdf
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Marc: |
LEADER 01003nam a2200289 a 4500 001 1595691 005 2013-04-24 008 2006 bl uuuu u0uu1 u #d 100 1 $aFERNANDES, E. N. 245 $aPolíticas ambientais aplicadas à questão do aquecimento global. 260 $aJuiz de Fora: Embrapa Gado de Leite$c2006 300 $a48 p. 490 $a(Embrapa Gado de Leite. Documentos, 115). 520 $aO problema ambiental: aquecimento global; As controvérsias; Dificuldades de tratamento das questões ambientais globais; Soluções propostas; As políticas ambientais. 650 $aAgricultura 650 $aEconomia 650 $aEducação Ambiental 650 $aMeio Ambiente 650 $aPolítica Ambiental 653 $aAquecimento global 653 $aAquecimento golobal 653 $aEconomia ecológica 653 $aInstrumento econômico 653 $aInstrumentos econômicos 653 $aQuestão ambiental 700 1 $aROMEIRO, A. R. 700 1 $aASSIS, C. R. de
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
Data corrente: |
29/05/2023 |
Data da última atualização: |
25/04/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
PEREIRA, B. M.; ARRAES, F.; MARTINS, A. C. Q.; ALVES, N. S. F.; MELO, B. P.; MORGANTE, C. V.; SARAIVA, M. A. P.; SA, M. F. G. de; GUIMARAES, P. M.; BRASILEIRO, A. C. M. |
Afiliação: |
BRUNA MEDEIROS PEREIRA, UNIVERSIDADE DE BRASÍLIA; FABRÍCIO ARRAES, UNIVERSIDADE DE BRASILIA; ANDRESSA CUNHA QUINTANA MARTINS, UNIVERSIDADE CATOLICA DE BRASILIA; NAYARA SABRINA FREITAS ALVES, UNIVERSIDADE FEDERAL DO PARANÁ; BRUNO PAES MELO, LONGPING HIGH TECH; CAROLINA VIANNA MORGANTE, CPATSA; MARIO ALFREDO PASSOS SARAIVA, INSTITUTO NACIONAL DE CIÊNCIA E TECNOLOGIA; MARIA FATIMA GROSSI DE SA, CENARGEN; PATRICIA MESSEMBERG GUIMARAES, CENARGEN; ANA CRISTINA MIRANDA BRASILEIRO, CENARGEN. |
Título: |
A novel soybean hairy root system for gene functional validation. |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
Plos One, v. 18, n. 5, e0285504, 2023. |
DOI: |
https://doi. org/10.1371/journal.pone.0285504 |
Idioma: |
Inglês |
Notas: |
Na publicação: Maria Fátima Grossi-de-Sa; Patricia Messenberg Guimaraes. |
Conteúdo: |
Agrobacterium rhizogenes-mediated transformation has long been explored as a versatile and reliable method for gene function validation in many plant species, including soybean (Glycine max). Likewise, detached-leaf assays have been widely used for rapid and mass screening of soybean genotypes for disease resistance. The present study combines these two methods to establish an efficient and practical system to generate transgenic soybean hairy roots from detached leaves and their subsequent culture under ex vitro conditions. We demonstrated that hairy roots derived from leaves of two (tropical and temperate) soybean cultivars could be successfully infected by economically important species of root-knot nematodes (Meloidogyne incognita and M. javanica). The established detached-leaf method was further explored for functional validation of two candidate genes encoding for cell wall modifying proteins (CWMPs) to promote resistance against M. incognita through distinct biotechnological strategies: the overexpression of a wild Arachis -expansin transgene (AdEXPA24) and the dsRNA-mediated silencing of an endogenous soybean polygalacturonase gene (GmPG). AdEXPA24 overexpression in hairy roots of RKN-susceptible soybean cultivar significantly reduced nematode infection by approximately 47%, whereas GmPG downregulation caused an average decrease of 37%. This novel system of hairy root induction from detached leaves showed to be an efficient, practical, fast, and low-cost method suitable for high throughput in root analysis of candidate genes in soybean. MenosAgrobacterium rhizogenes-mediated transformation has long been explored as a versatile and reliable method for gene function validation in many plant species, including soybean (Glycine max). Likewise, detached-leaf assays have been widely used for rapid and mass screening of soybean genotypes for disease resistance. The present study combines these two methods to establish an efficient and practical system to generate transgenic soybean hairy roots from detached leaves and their subsequent culture under ex vitro conditions. We demonstrated that hairy roots derived from leaves of two (tropical and temperate) soybean cultivars could be successfully infected by economically important species of root-knot nematodes (Meloidogyne incognita and M. javanica). The established detached-leaf method was further explored for functional validation of two candidate genes encoding for cell wall modifying proteins (CWMPs) to promote resistance against M. incognita through distinct biotechnological strategies: the overexpression of a wild Arachis -expansin transgene (AdEXPA24) and the dsRNA-mediated silencing of an endogenous soybean polygalacturonase gene (GmPG). AdEXPA24 overexpression in hairy roots of RKN-susceptible soybean cultivar significantly reduced nematode infection by approximately 47%, whereas GmPG downregulation caused an average decrease of 37%. This novel system of hairy root induction from detached leaves showed to be an efficient, practical, fast, and low-cost method suita... Mostrar Tudo |
Palavras-Chave: |
Agrobacterium rhizogenes; Transgenic soybean. |
Thesagro: |
Glycine Max; Melhoramento Genético Vegetal; Meloidogyne spp; Soja. |
Thesaurus NAL: |
Soybeans. |
Categoria do assunto: |
-- G Melhoramento Genético |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1154066/1/A-novel-soybean-hairy-root-system-for-gene-functional-validation-2023.pdf
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Marc: |
LEADER 02598naa a2200337 a 4500 001 2154066 005 2024-04-25 008 2023 bl uuuu u00u1 u #d 024 7 $ahttps://doi. org/10.1371/journal.pone.0285504$2DOI 100 1 $aPEREIRA, B. M. 245 $aA novel soybean hairy root system for gene functional validation.$h[electronic resource] 260 $c2023 500 $aNa publicação: Maria Fátima Grossi-de-Sa; Patricia Messenberg Guimaraes. 520 $aAgrobacterium rhizogenes-mediated transformation has long been explored as a versatile and reliable method for gene function validation in many plant species, including soybean (Glycine max). Likewise, detached-leaf assays have been widely used for rapid and mass screening of soybean genotypes for disease resistance. The present study combines these two methods to establish an efficient and practical system to generate transgenic soybean hairy roots from detached leaves and their subsequent culture under ex vitro conditions. We demonstrated that hairy roots derived from leaves of two (tropical and temperate) soybean cultivars could be successfully infected by economically important species of root-knot nematodes (Meloidogyne incognita and M. javanica). The established detached-leaf method was further explored for functional validation of two candidate genes encoding for cell wall modifying proteins (CWMPs) to promote resistance against M. incognita through distinct biotechnological strategies: the overexpression of a wild Arachis -expansin transgene (AdEXPA24) and the dsRNA-mediated silencing of an endogenous soybean polygalacturonase gene (GmPG). AdEXPA24 overexpression in hairy roots of RKN-susceptible soybean cultivar significantly reduced nematode infection by approximately 47%, whereas GmPG downregulation caused an average decrease of 37%. This novel system of hairy root induction from detached leaves showed to be an efficient, practical, fast, and low-cost method suitable for high throughput in root analysis of candidate genes in soybean. 650 $aSoybeans 650 $aGlycine Max 650 $aMelhoramento Genético Vegetal 650 $aMeloidogyne spp 650 $aSoja 653 $aAgrobacterium rhizogenes 653 $aTransgenic soybean 700 1 $aARRAES, F. 700 1 $aMARTINS, A. C. Q. 700 1 $aALVES, N. S. F. 700 1 $aMELO, B. P. 700 1 $aMORGANTE, C. V. 700 1 $aSARAIVA, M. A. P. 700 1 $aSA, M. F. G. de 700 1 $aGUIMARAES, P. M. 700 1 $aBRASILEIRO, A. C. M. 773 $tPlos One$gv. 18, n. 5, e0285504, 2023.
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Embrapa Semiárido (CPATSA) |
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