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Registro Completo |
Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
12/12/2018 |
Data da última atualização: |
24/01/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
WOHLRES-VIANA, S.; ARASHIRO, E. K. N.; MINARE, T. P.; FERNANDES, C. A. C.; GRAZIA, J. G. V.; SIQUEIRA, L. G. B.; MACHADO, M. A.; VIANA, J. H. M. |
Afiliação: |
S. WOHLRES-VIANA, UFJF; E. K. N. ARASHIRO, UFF; T. P. MINARE, Universidade José do Rosario Vellano, Alfenas, MG; C. A. C. FERNANDES, Universidade Jose do Rosario Vellano / Biotran Biotecnologia Animal, Alfenas, MG; J. G. V. GRAZIA, UFMG; LUIZ GUSTAVO BRUNO SIQUEIRA, CNPGL; MARCO ANTONIO MACHADO, CNPGL; JOAO HENRIQUE MOREIRA VIANA, Cenargen. |
Título: |
Differential expression of LHCGR and its isoforms is associated to the variability in superovulation responses of Gir cattle. |
Ano de publicação: |
2019 |
Fonte/Imprenta: |
Theriogenology, v. 126, p. 68-74, 2019. |
DOI: |
10.1016/j.theriogenology.2018.12.004 |
Idioma: |
Inglês |
Conteúdo: |
Abstract The aim of this study was to evaluate the pattern of expression of LHCGR isoforms in Gir heifers characterized as good (10.3 ± 1.2 ova/embryos per flush, n = 5) or poor responders (1.1 ± 0.3 ova/embryos per flush, n = 5) to superovulation protocols. In both groups, an adapted ultrasound-guided follicular aspiration system was used to collect granulosa cells from 8 mm follicles formed either during a synchronized, non-stimulated follicular wave (no stimulation control, NS) or on the fourth day of a superovulation protocol (SOV) induced with 200 IU of pFSH. The recovered follicular fluid was centrifuged and granulosa cells were washed with NaCl 0.9% and kept in RNAlater®. RNA extraction was performed using a commercial RNeasy Micro Kit and eluted samples were quantified and reverse transcribed using the commercial Superscript III kit. cDNA samples were amplified by real-time PCR using a primer to target LH/hCG receptor gene - not selective for LHCGR isoforms (total LHCGR) - and four sets of isoforms selective primers (S1, S10, S10 + 11, and S11). Analyses were performed using the REST software and expression levels are shown as mean ± SEM. Under physiological conditions (NS), poor responders had a higher expression of total LHCGR (4.9 ± 1.7 fold-change, P < 0.01) as well as isoforms S10, S11 and S10 + 11, compared to good responders. In both phenotypes, superovulation down-regulated total LHCGR expression (-0.5 ± 0.2 and -0.9 ± 0.0 for good and poor responders, respectively; P < 0.05). However, in poor responders the exogenous FSH treatment up-regulated the S10 (2.4 ± 2.0; P < 0.05), S10 + 11 (3.8 ± 3.2; P < 0.01), and S1 isoforms (1.8 ± 1.3; P < 0.05), compared to good responders We conclude that down-regulation of total LHCGR, associated to up-regulation of their inactive isoforms, may have compromised follicle development and thus contributed to the low efficiency of superovulation in heifers with a poor responder phenotype. MenosAbstract The aim of this study was to evaluate the pattern of expression of LHCGR isoforms in Gir heifers characterized as good (10.3 ± 1.2 ova/embryos per flush, n = 5) or poor responders (1.1 ± 0.3 ova/embryos per flush, n = 5) to superovulation protocols. In both groups, an adapted ultrasound-guided follicular aspiration system was used to collect granulosa cells from 8 mm follicles formed either during a synchronized, non-stimulated follicular wave (no stimulation control, NS) or on the fourth day of a superovulation protocol (SOV) induced with 200 IU of pFSH. The recovered follicular fluid was centrifuged and granulosa cells were washed with NaCl 0.9% and kept in RNAlater®. RNA extraction was performed using a commercial RNeasy Micro Kit and eluted samples were quantified and reverse transcribed using the commercial Superscript III kit. cDNA samples were amplified by real-time PCR using a primer to target LH/hCG receptor gene - not selective for LHCGR isoforms (total LHCGR) - and four sets of isoforms selective primers (S1, S10, S10 + 11, and S11). Analyses were performed using the REST software and expression levels are shown as mean ± SEM. Under physiological conditions (NS), poor responders had a higher expression of total LHCGR (4.9 ± 1.7 fold-change, P < 0.01) as well as isoforms S10, S11 and S10 + 11, compared to good responders. In both phenotypes, superovulation down-regulated total LHCGR expression (-0.5 ± 0... Mostrar Tudo |
Palavras-Chave: |
In vivo embryo production. |
Thesaurus Nal: |
Embryo transfer; Luteinizing hormone. |
Categoria do assunto: |
-- L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 02857naa a2200253 a 4500 001 2106166 005 2023-01-24 008 2019 bl uuuu u00u1 u #d 024 7 $a10.1016/j.theriogenology.2018.12.004$2DOI 100 1 $aWOHLRES-VIANA, S. 245 $aDifferential expression of LHCGR and its isoforms is associated to the variability in superovulation responses of Gir cattle.$h[electronic resource] 260 $c2019 520 $aAbstract The aim of this study was to evaluate the pattern of expression of LHCGR isoforms in Gir heifers characterized as good (10.3 ± 1.2 ova/embryos per flush, n = 5) or poor responders (1.1 ± 0.3 ova/embryos per flush, n = 5) to superovulation protocols. In both groups, an adapted ultrasound-guided follicular aspiration system was used to collect granulosa cells from 8 mm follicles formed either during a synchronized, non-stimulated follicular wave (no stimulation control, NS) or on the fourth day of a superovulation protocol (SOV) induced with 200 IU of pFSH. The recovered follicular fluid was centrifuged and granulosa cells were washed with NaCl 0.9% and kept in RNAlater®. RNA extraction was performed using a commercial RNeasy Micro Kit and eluted samples were quantified and reverse transcribed using the commercial Superscript III kit. cDNA samples were amplified by real-time PCR using a primer to target LH/hCG receptor gene - not selective for LHCGR isoforms (total LHCGR) - and four sets of isoforms selective primers (S1, S10, S10 + 11, and S11). Analyses were performed using the REST software and expression levels are shown as mean ± SEM. Under physiological conditions (NS), poor responders had a higher expression of total LHCGR (4.9 ± 1.7 fold-change, P < 0.01) as well as isoforms S10, S11 and S10 + 11, compared to good responders. In both phenotypes, superovulation down-regulated total LHCGR expression (-0.5 ± 0.2 and -0.9 ± 0.0 for good and poor responders, respectively; P < 0.05). However, in poor responders the exogenous FSH treatment up-regulated the S10 (2.4 ± 2.0; P < 0.05), S10 + 11 (3.8 ± 3.2; P < 0.01), and S1 isoforms (1.8 ± 1.3; P < 0.05), compared to good responders We conclude that down-regulation of total LHCGR, associated to up-regulation of their inactive isoforms, may have compromised follicle development and thus contributed to the low efficiency of superovulation in heifers with a poor responder phenotype. 650 $aEmbryo transfer 650 $aLuteinizing hormone 653 $aIn vivo embryo production 700 1 $aARASHIRO, E. K. N. 700 1 $aMINARE, T. P. 700 1 $aFERNANDES, C. A. C. 700 1 $aGRAZIA, J. G. V. 700 1 $aSIQUEIRA, L. G. B. 700 1 $aMACHADO, M. A. 700 1 $aVIANA, J. H. M. 773 $tTheriogenology$gv. 126, p. 68-74, 2019.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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Registros recuperados : 73 | |
3. | | FERREIRA, A. M.; SA, W. F.; VIANA, J. H. M.; FERNANDES, C. A. C.; CAMARGO, L. S. A. Taxa de nao retorno ao cio em 60 dias e taxa de prenhez em bovinos. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Belo Horizonte, v. 49, n. 2, p. 261-267, 1997.Biblioteca(s): Embrapa Gado de Leite. |
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4. | | MIYAUCHI, C. A. C.; FERNANDES, C. A. C.; OLIVEIRA, E. R.; ALVES, B. F. L.; VIANA, J. H. M. Effect of follicular wave synchronization and absence of corpus luteum on coc recovery in gyr (Bos taurus indicus) cows. Reproduction, Fertility and Development, v. 22, n. 1, 2010. Edição dos proceedings do Annual Conference of the International Embryo, 2010, Cordoba.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Gado de Leite. |
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5. | | VIANA, J. H. M.; PEREIRA, N. E. S.; FARIA, O. A. C.; DIAS, L. R. O.; OLIVEIRA, E. R.; FERNANDES, C. A. C.; SIQUEIRA, L. G. B. Active immunization against GnRH as an alternative therapeutic approach for the management of Bos indicus oocyte donors diagnosed with chronic cystic ovarian disease. Theriogenology, v. 172, p. 133-141, 2021.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia. |
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6. | | VARAGO, F. C.; SILVA, L. P.; RIBEIRO, J. R.; FERNANDES, C. A. C.; CARVALHO, B. C. de; GIOSO, M. M.; GIMENEZ, M.; NEVES, J. P. Acrosome reaction induced by theophylline associated or not to heparin in bovine semen Animal Reproduction, v. 12, n. 3, 2015. Edição dos proceedings do 29º Annual Meeting of the Brazilian Embryo Technology Society, 2015, Gramado.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Gado de Leite. |
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7. | | GUIMARÃES, C. R. B.; OLIVEIRA, M. E.; ROSSI, J. R.; FERNANDES, C. A. C.; VIANA, J. H. M.; PALHAO, M. P. Corpus luteum blood flow evaluation on Day 21 to improve the management of embryo recipient herds. Theriogenology, v. 84, n. 2, p. 237-241, 2015.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Gado de Leite. |
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8. | | ROSSI, T. C.; CAMARGO, L. S. de A.; FERNANDES, C. A. C.; VARAGO, F. C.; MIYAUCHI, T. M.; ROSSI, J. R.; GIOSO, M. M. Bovine oocyte transportation in environment with or without control gas atmosphere. Animal Reproduction, v. 10, n. 3, p. 547, Jul./Sept. 2013. Suplemento. Edição dos abstracts do 27º Annual Meeting of the Brazilian Embryo Technology Society, 2013, Praia do Forte, BA.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Gado de Leite. |
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9. | | FERNANDES, C. A. C.; OLIVEIRA, E. R.; VASCONCELOS, T. D.; ALVES, B. F. L.; GIOSO, M. M.; VIANA, J. H. M.; OBA, E.; FIGUEIREDO, A. C. S. Bipartição de embriões bovinos em diferentes estádios de desenvolvimento. Acta Scientiae Veterinariae, v. 35, p. s1215, 2007. Supl. 3. Edição dos anais da 21ª Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Salvador, 2007.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Gado de Leite. |
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10. | | VIANA, J. H. M.; CAMARGO, L. S. A.; SIQUEIRA, L. G. B.; SOUZA, E. D.; FREITAS, C.; FERNANDES, C. A. C. Characteristics of the superovulatory response in Gyr (Bos Indicus) cattle. Reproduction, Fertility and Development, v. 19, n. 1, p. 311-312, 2007. Edição dos proceedings do Annual Conference of the Internatinal Embryo Transfer Society, 2007, Kyoto.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Gado de Leite. |
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11. | | VIANA, J. H. M.; SIQUEIRA, L. G. B.; DINIZ, E. S.; CAMARGO, L. S. A.; OLIVEIRA, E. R.; FONSECA, J. F.; FERNANDES, C. A. C. Definição de aréa mínima representativa para análise de imagens ultra-sonográficas de corpos lúteos bovinos. Acta Scientiae Veterinariae, Porto Alegre, v. 34, supl. 1, p. s578, 2006. Edição dos resumos da 20. Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Araxá, MG, agosto 2006.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Caprinos e Ovinos. |
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12. | | VIANA, J. H. M.; SIQUEIRA, L. G. B.; DINIZ, E. S.; CAMARGO, L. S. A.; OLIVEIRA, E. R.; FONSECA, J. F.; FERNANDES, C. A. C. Definition of minimum representative area for sonographic bovine corpus luteum image analysis. Acta Scientiae Veterinariae, v. 34, s578, 2006. Supl. 1. Edição dos resumos da 20ª Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Araxá, 2006.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Gado de Leite. |
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13. | | FERNANDES, C. A. C.; OBA, E.; VIANA, J. H. M.; FIGUEIREDO, A. C. S.; OLIVEIRA, E. R.; VASCONCELOS, T. D.; GIOSO, M. M. Economic evaluation of bovine embryo splitting. Acta Scientiae Veterinariae, v. 34, s506, 2006. Supl. 1. Edição dos resumos da 20ª Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Araxá, 2006.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Gado de Leite. |
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14. | | PALHAO, M. P.; OLIVEIRA, E. R.; GIOSO, M. M.; CARVALHO, B. C. de; SIQUEIRA, L. G. B.; FERNANDES, C. A. C.; VIANA, J. H. M. The relationship between oocyte recovery and embryo production in Bos indicus. In: ANNUAL CONFERENCE OF THE INTERNACIONAL EMBRYO TRANSFES SOCIETY, 2012, Phoenix. Proceedings... [S.l.:s.n.], 2012.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Gado de Leite. |
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15. | | VIANA, J. H. M.; PALHÃO, M. P.; ARASHIRO, E. K. N.; FERREIRA, A. M.; FONSECA, J. F. da; FERNANDES, C. A. C. Pre-sincronização de vacas para coleta de complexos cumulus-oócito: resultados parciais. Acta Scientiae Veterinariae, Porto Alegre, v. 32, p. 187, 2004. Suplemento.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Caprinos e Ovinos. |
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16. | | VIANA, J. H. M.; CAMARGO, L. S. A.; FONSECA, J. F.; OLIVEIRA, A. P.; ARASHIRO, E. K. N.; FREITAS, C.; FERNANDES, C. A. C. Retrospective analysis of cumulus-oocyte complexyield in a gyr (Bos Taurus Indicus) herd undergoing trasvaginal ultrasound-guided fillicle aspiration. In: REPRODUCTION, FERTILITY AND DEVELOPMENT. 20. 2008, Anais. Colorado. Csiro, Academy of Science, 2008. 1 p.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Gado de Leite. |
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17. | | VIANA, J. H. M.; CAMARGO, L. S. H.; FONSECA, J. F. da; OLIVEIRA, A. P.; ARASCHIRO, E. K. N.; FREITAS, C.; FERNANDES, C. A. C. Retrospective analysis of cumulus-oocyte complex yield ina GYR (Bos Taurus indicus) herd undergoing transvaginal ultrasound-guided follicle aspiration. Reproduction, Fertility and Development, v. 20, n. 1, p. 189, 2008. Edição dos proceedings da Annual Conference or the International Embryo Transfer Society, Denver, Colorado, 2008.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Caprinos e Ovinos. |
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18. | | ARASHIRO, E. K. N.; SIQUEIRA, L. G. B.; VIANA, J. H. M.; FONSECA, J. F.; BRUSCHI, J. H.; CAMARGO, L. S.; FERNANDES, C. A. C.; BRANDÃO, F. Z. Estudo ultra-sonográfico de características luteais no período de luteogênese em cabras da raça Toggenburg. Acta Scientiae Veterinariae, v. 35, p. s1291, 2007. Supl. 3. Edição dos anais da 21ª Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Salvador, 2007.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Caprinos e Ovinos; Embrapa Gado de Leite. |
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19. | | PALHÃO, M. P.; JUNIOR, N. S.; GUIMARÃES, C. R. B.; FERNANDES, C. A. C.; FERREIRA, M. E. O.; SEBER, M.; REIS, W. S. M.; VIANA, J. H. M. Follicular growth and blood flow of the dominant follicle in crossbred recipients treated with eCG. In: ANNUAL CONFERENCE OF THE INTERNATIONAL EMBRYO TRANSFER SOCIETY, 2013, Hannover. Proceedings... Hannover: [s.n.], 2013.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Gado de Leite. |
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20. | | FERNANDES, C. A. C.; FIGUEIREDO, A. C. S.; OLIVEIRA, E. R.; VASCONCELOS, T. D.; ALVES, B. F. L.; GIOSO, M. M.; VIANA, J. H. M. Folltropin-V® dose trial on superovulation protocol in european beef cows. Acta Scientiae Veterinariae, v. 34, s514, 2006. Supl. 1. Edição dos resumos da 20ª Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Araxá, 2006.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Gado de Leite. |
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Registros recuperados : 73 | |
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Nenhum registro encontrado para a expressão de busca informada. |
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