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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
15/04/2004 |
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Data da última atualização: |
15/04/2004 |
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Autoria: |
SCHÖPKE, C.; TAYLOR, N. J.; MASONA, M. V.; CÁRCAMO, R.; HO, T.; BEACHY, R. N.; FAUQUET, C. M. |
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Título: |
Characterization of transgenic cassava plants containing the coat protein gene of cassava common mosaic virus. |
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Ano de publicação: |
1998 |
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Fonte/Imprenta: |
Revista Brasileira de Mandioca, Cruz das Almas, v. 17, p. 33, nov., 1998. Suplemento. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: During recent years several groups were successful in genetically transforming cassava. While initially marker genes were used to optimize transformation protocols, work is now focusing on the transfer of genes of interest into this crop. One of our goals is to apply the coat protein-mediated resistance strategy to produce cassava plants resistant to a virus originating from South America, cassava common mosaic virus (CsCMV). Gene constructs containing the neophosphomycin transferase gene (nptll) as selectable marker and the coat protein gene of CsCMV were transferred through particle bombardment into embryogenic suspension - derived tissue of cassava cultivar TMS 60444. Bombarded tissues were selected with paromomycin, and after regeneration of selected tissue through somatic embryogenesis eventually more than thirty transgenic plant lines were established. CsCMV coat protein was detected through Western blot analysis in nine of these lines. Data will be presented on the production of the transgenic plants and on their general and molecular characterization. |
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Categoria do assunto: |
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Marc: |
LEADER 01677naa a2200193 a 4500
001 1651910
005 2004-04-15
008 1998 bl uuuu u00u1 u #d
100 1 $aSCHÖPKE, C.
245 $aCharacterization of transgenic cassava plants containing the coat protein gene of cassava common mosaic virus.
260 $c1998
520 $aAbstract: During recent years several groups were successful in genetically transforming cassava. While initially marker genes were used to optimize transformation protocols, work is now focusing on the transfer of genes of interest into this crop. One of our goals is to apply the coat protein-mediated resistance strategy to produce cassava plants resistant to a virus originating from South America, cassava common mosaic virus (CsCMV). Gene constructs containing the neophosphomycin transferase gene (nptll) as selectable marker and the coat protein gene of CsCMV were transferred through particle bombardment into embryogenic suspension - derived tissue of cassava cultivar TMS 60444. Bombarded tissues were selected with paromomycin, and after regeneration of selected tissue through somatic embryogenesis eventually more than thirty transgenic plant lines were established. CsCMV coat protein was detected through Western blot analysis in nine of these lines. Data will be presented on the production of the transgenic plants and on their general and molecular characterization.
700 1 $aTAYLOR, N. J.
700 1 $aMASONA, M. V.
700 1 $aCÁRCAMO, R.
700 1 $aHO, T.
700 1 $aBEACHY, R. N.
700 1 $aFAUQUET, C. M.
773 $tRevista Brasileira de Mandioca, Cruz das Almas$gv. 17, p. 33, nov., 1998. Suplemento.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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