Data/hora: 03/06/2024 01:05:45

Biblioteca(s): | Embrapa Mandioca e Fruticultura.
Data corrente: | 22/04/1994
Data da última atualização: | 22/04/1994
Autoria: | FAUQUET, C. M.; SCHOPKE, C.; CHAVARRIAGA, P.; SANGARE, A.; VEACHY, R. N.
Afiliação: | ILTAB/TSRI, La Jolla, CA, USA.
Título: | Genetic engineering technologies to control viruses and their application to cassava viruses.
Ano de publicação: | 1993
Fonte/Imprenta: | Cali, Colombia: CIAT, 1993
Páginas: | 190-207p
Série: | CIAT,Working Document, 123
Idioma: | Inglês
Notas: | International scientific meeting Cassava Biotecnology Network, 1, 1992, Cartajena, Colombia. Proceedings... Cali, Colombia: CIAT, 1993.
Conteúdo: | Resistance to plant viruses through genetic engineering has been investigated with the application of four different approaches. A recent approach is the use of rigozymes, which involves using catalytic RNAs to cause the cleavage of viral RNA molecules. Results of in vitro experiments have documented cleavafe of viral RNAs but in vivo experiments has thus far not been reported. The second tecriques is to attempt to block the translation of viral RNAs by wxprossing genes that encode sequences complementary (i.e. antisense) to viral genes. Only a limited numger of cases have been reported, but little success has been documented. A third approach is to block virus replication, using competitor sequences, subgenomic sequences  and satellite RNAs. The last techniq ue is the expression of viral genes which will interfere with virus replication and/or, diffusion in the plants; replicase gene, movement proteins and capsid proteins. The most promising techmique is that of the coat protein (CP), which consists of the integration of a gene encoding viral coat protein into the plant genome. An increasing number of examples using the coat protein mediated resistance (CP _ MR) strategy are avaliable, and resistance specificity and effecacy have been evaluated for viruses belonging to thirteen groups; furthermore, several successful field wxperiments have been conducted.
Palavras-Chave: | Casa de vegetacao; Congresso.
Thesagro: | Genoma; Vírus.
Categoria do assunto: | --
Registro original: | Embrapa Mandioca e Fruticultura (CNPMF)
Exemplares: | CNPMF - (13433-1) - ADD - PL - -- - 633.6820601 - I61p - 199300007 - DIS