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 | Acesso ao texto completo restrito à biblioteca da Embrapa Amazônia Oriental. Para informações adicionais entre em contato com cpatu.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Amazônia Oriental. |
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Data corrente: |
13/03/2014 |
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Data da última atualização: |
19/10/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FARIA, M. T. de; CURY-BOAVENTURA, M. F.; LOPES, L. R.; SILVA, J. R. M. C. da. |
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Afiliação: |
MARCOS TUCUNDUVA DE FARIA, CPATU; Maria Fernanda Cury-Boaventura, USP; Lucia Rossetti Lopes, USP; José Roberto Machado Cunha da Silva, USP. |
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Título: |
Generation of reactive oxygen species by leukocytes of Prochilodus lineatus. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Fish Physiology and Biochemistry, v. 40, n. 2, p. 445-455, Apr. 2014. |
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DOI: |
10.1007/s10695-013-9856-9 |
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Idioma: |
Inglês |
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Conteúdo: |
Prochilodus lineatus (curimbatá), from the Procholodontidae family, is a Brazilian freshwater fish, which is important commercially, nutritionally and ecologically. It is encountered in the Rio da Prata Bay in Southern South America. Studies on the immune system of this fish are scarce, but the physiological mechanisms of the species are analogous to those of other vertebrates. Thus, this work discusses the present study, which correlates P. lineatus leukocytes and the generation of reactive oxygen species after modulatory stimuli. Leukocytes were characterized by light and electron transmission microscopy and investigated by the generation of H2O2 and O2 −, using phenol red, flow-cytometry and electron transmission histochemistry. The study determined that monocytes and neutrophils are the main cells responsible for generating O2 after stimulation with phorbol myristate acetate. Superoxide dismutase successfully inhibited the generation of reactive oxygen species in neutrophils and monocytes, but stimulated generation when in association with phorbol myristate acetate. Fish leukocyte samples from P. lineatus showed cross-reactivity with antibodies directed against human NADPH-oxidase antibody subunits (p47phox and p67phox). Thus, catalase enhanced the presence of p47phox. Neutrophil mitochondria were shown to be generators of H2O2 (charged by cerium precipitate), being enlarged and changing their format. The present study contributes to a better understanding of the respiratory burst pathways in this species and suggests mitochondria as the organelle responsible for generation of reactive oxygen species. MenosProchilodus lineatus (curimbatá), from the Procholodontidae family, is a Brazilian freshwater fish, which is important commercially, nutritionally and ecologically. It is encountered in the Rio da Prata Bay in Southern South America. Studies on the immune system of this fish are scarce, but the physiological mechanisms of the species are analogous to those of other vertebrates. Thus, this work discusses the present study, which correlates P. lineatus leukocytes and the generation of reactive oxygen species after modulatory stimuli. Leukocytes were characterized by light and electron transmission microscopy and investigated by the generation of H2O2 and O2 −, using phenol red, flow-cytometry and electron transmission histochemistry. The study determined that monocytes and neutrophils are the main cells responsible for generating O2 after stimulation with phorbol myristate acetate. Superoxide dismutase successfully inhibited the generation of reactive oxygen species in neutrophils and monocytes, but stimulated generation when in association with phorbol myristate acetate. Fish leukocyte samples from P. lineatus showed cross-reactivity with antibodies directed against human NADPH-oxidase antibody subunits (p47phox and p67phox). Thus, catalase enhanced the presence of p47phox. Neutrophil mitochondria were shown to be generators of H2O2 (charged by cerium precipitate), being enlarged and changing their format. The present study contributes to a better understanding of the r... Mostrar Tudo |
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Palavras-Chave: |
Citometria de fluxo; Espécies reativas de oxigênio; Explosão respiratória; Mitocôndrias. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 02340naa a2200217 a 4500
001 1982284
005 2022-10-19
008 2014 bl uuuu u00u1 u #d
024 7 $a10.1007/s10695-013-9856-9$2DOI
100 1 $aFARIA, M. T. de
245 $aGeneration of reactive oxygen species by leukocytes of Prochilodus lineatus.$h[electronic resource]
260 $c2014
520 $aProchilodus lineatus (curimbatá), from the Procholodontidae family, is a Brazilian freshwater fish, which is important commercially, nutritionally and ecologically. It is encountered in the Rio da Prata Bay in Southern South America. Studies on the immune system of this fish are scarce, but the physiological mechanisms of the species are analogous to those of other vertebrates. Thus, this work discusses the present study, which correlates P. lineatus leukocytes and the generation of reactive oxygen species after modulatory stimuli. Leukocytes were characterized by light and electron transmission microscopy and investigated by the generation of H2O2 and O2 −, using phenol red, flow-cytometry and electron transmission histochemistry. The study determined that monocytes and neutrophils are the main cells responsible for generating O2 after stimulation with phorbol myristate acetate. Superoxide dismutase successfully inhibited the generation of reactive oxygen species in neutrophils and monocytes, but stimulated generation when in association with phorbol myristate acetate. Fish leukocyte samples from P. lineatus showed cross-reactivity with antibodies directed against human NADPH-oxidase antibody subunits (p47phox and p67phox). Thus, catalase enhanced the presence of p47phox. Neutrophil mitochondria were shown to be generators of H2O2 (charged by cerium precipitate), being enlarged and changing their format. The present study contributes to a better understanding of the respiratory burst pathways in this species and suggests mitochondria as the organelle responsible for generation of reactive oxygen species.
653 $aCitometria de fluxo
653 $aEspécies reativas de oxigênio
653 $aExplosão respiratória
653 $aMitocôndrias
700 1 $aCURY-BOAVENTURA, M. F.
700 1 $aLOPES, L. R.
700 1 $aSILVA, J. R. M. C. da
773 $tFish Physiology and Biochemistry$gv. 40, n. 2, p. 445-455, Apr. 2014.
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Embrapa Amazônia Oriental (CPATU) |
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| 10. |  | MAKISHIMA, N. HORTALIÇAS cultivo a campo ou em estufas? Revista Campo & Negócios HF, Uberlândia, v. 2, n. 21, p. 44-46, fev. 2007. Informações prestadas por Nozomu Makishima, pesquisadopr da Embrapa Hortaliças.| Tipo: Artigo de Divulgação na Mídia |
| Biblioteca(s): Embrapa Hortaliças. |
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| 14. | | MAKISHIMA, N. Cultivo de hortalicas. 2.ed. Brasilia: EMBRAPA-CNPH, 1992. 26p. (EMBRAPA-CNPH. Instrucoes Tecnicas da Embrapa Hortalicas, 6).| Biblioteca(s): Embrapa Hortaliças. |
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| 15. | | MAKISHIMA, N. O cultivo de hortaliças. Brasília, DF: Embrapa-SPI: Embrapa-CNPH, 1993. 108 p. (Coleção Plantar, 4).| Tipo: Autoria/Organização/Edição de Livros |
| Biblioteca(s): Embrapa Hortaliças; Embrapa Unidades Centrais. |
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| Registros recuperados : 130 | |
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