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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia; Embrapa Cerrados. |
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Data corrente: |
27/06/1995 |
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Data da última atualização: |
29/09/2017 |
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Autoria: |
GILBERTSON, R. L.; HIDAYAT, S. H.; MARTINEZ, R. T.; LEONG, S. A.; FARIA, J. C.; MORALES, F.; MAXWELL, D. P. |
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Título: |
Differentiation of bean-infecting geminiviruses by nucleic acid hybridization probes and aspects of bean golden mosaic in Brazil. |
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Ano de publicação: |
1991 |
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Fonte/Imprenta: |
Plant Disease, St.Paul, v. 75, n. 4, p. 336-342, Apr. 1991. |
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Idioma: |
Inglês
Português |
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Conteúdo: |
A bean golden mosaic geminiviral isolate from Goiania, Goias, Brazil (BGMV-BZ), was determined to be transmitted by whiteflies and induced golden mosaic symptoms and diagnostic geminiviral inclusion bodies and ultrastructural abnormalities in infected bean leaves. In contrast to BGMV isolates from Central America and the Caribbean, BGMV-BZ could not be sap-transmitted to beans. Cloned geminiviral DNA components were used as DNA probes for the rapid and specific detection of BGMV-BZ and three sap-transmissible bean-infecting geminiviral isolates - BGMV from Guatemala (BGMV-GA) and the Dominican Republic (BGMV-DR; and bean dwarf mosaic geminivirus from Colombia (BDMV-CO). A general DNA probe detected all four viral isolates, whereas specific probes detected BGMV-BZ, BDMV, or BGMV-GA and BGMV-DR. Nucleic acid dot and squash blot methods were used to prepare samples for hybridization, and the dot blot method was used to determine relative differences in viral nucleic acid titers in infected bean leaves. The general and specific probes were emplyed to study the variability of BGMV isolates and potential weed reservoirs of geminiviruses in the Dominican Republic. |
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Palavras-Chave: |
Bean. |
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Thesagro: |
Ácido Nucléico; Cerrado; Feijão; Mosaico Dourado; Phaseolus Vulgaris; Vírus. |
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Thesaurus Nal: |
nucleic acids; viruses. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02017naa a2200301 a 4500
001 1552300
005 2017-09-29
008 1991 bl --- 0-- u #d
100 1 $aGILBERTSON, R. L.
245 $aDifferentiation of bean-infecting geminiviruses by nucleic acid hybridization probes and aspects of bean golden mosaic in Brazil.
260 $c1991
520 $aA bean golden mosaic geminiviral isolate from Goiania, Goias, Brazil (BGMV-BZ), was determined to be transmitted by whiteflies and induced golden mosaic symptoms and diagnostic geminiviral inclusion bodies and ultrastructural abnormalities in infected bean leaves. In contrast to BGMV isolates from Central America and the Caribbean, BGMV-BZ could not be sap-transmitted to beans. Cloned geminiviral DNA components were used as DNA probes for the rapid and specific detection of BGMV-BZ and three sap-transmissible bean-infecting geminiviral isolates - BGMV from Guatemala (BGMV-GA) and the Dominican Republic (BGMV-DR; and bean dwarf mosaic geminivirus from Colombia (BDMV-CO). A general DNA probe detected all four viral isolates, whereas specific probes detected BGMV-BZ, BDMV, or BGMV-GA and BGMV-DR. Nucleic acid dot and squash blot methods were used to prepare samples for hybridization, and the dot blot method was used to determine relative differences in viral nucleic acid titers in infected bean leaves. The general and specific probes were emplyed to study the variability of BGMV isolates and potential weed reservoirs of geminiviruses in the Dominican Republic.
650 $anucleic acids
650 $aviruses
650 $aÁcido Nucléico
650 $aCerrado
650 $aFeijão
650 $aMosaico Dourado
650 $aPhaseolus Vulgaris
650 $aVírus
653 $aBean
700 1 $aHIDAYAT, S. H.
700 1 $aMARTINEZ, R. T.
700 1 $aLEONG, S. A.
700 1 $aFARIA, J. C.
700 1 $aMORALES, F.
700 1 $aMAXWELL, D. P.
773 $tPlant Disease, St.Paul$gv. 75, n. 4, p. 336-342, Apr. 1991.
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Registro original: |
Embrapa Cerrados (CPAC) |
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