Data/hora: 04/06/2024 06:28:58

Biblioteca(s): 	Embrapa Arroz e Feijão; Embrapa Recursos Genéticos e Biotecnologia.	
Data corrente: 	30/04/1997	
Data da última atualização: 	19/10/2007	
Autoria: 	ARAGÃO, F. J. L.; BARROS, L. M. G.; BRASILEIRO, A. C. M.; RIBEIRO, S. G.; SMITH, F. D.; SANFORD, J. C.; FARIA, J. C.; RECH, E. L.	
Afiliação: 	EMBRAPA-CENARGEN.	
Título: 	Inheritance of foreign genes in transgenic bean (Phaseolus vulgaris L.) co-transformed via particle bombardment.	
Ano de publicação: 	1996	
Fonte/Imprenta: 	Theoretical and Applied Genetics, Berlin, v.93, n.l/2, p.142-150, l996.	
Páginas: 	p. 142-150	
Idioma: 	Inglês	
Conteúdo: 	Exploiting the biolistic process we have generated stable transgenic bean (Phaseolus vulgaris L.) plants with unlinked and linked foreign genes. Co-transformation was conducted using plasmid constructions containing a fusion of the gus and neo genes, which were co-introduced with the methionine-rich 2S albumin gene isolated from the Brazil nut and the antisense sequence of AC1, AC2, AC3 and BC1 genes from the bean golden mosaic geminivirus. The results revealed a co-transformation frequency ranging from 40% to 50% when using unlinked genes and 100% for linked genes. The introduced foreign genes were inherited in a Mendelian fashion in most of the transgenic bean lines. PCR and Southern blot hybridization confirmed the integration of the foreign genes in the plant genome.	
Palavras-Chave: 	Bean; Biolistica; Electrical; Feijão transgênico; Particle; Transgenic bean.	
Thesagro: 	Feijão; Phaseolus Vulgaris.	
Thesaurus Nal: 	biolistics.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Recursos Genéticos e Biotecnologia (CENARGEN)	
Exemplares: 	CENARGEN - (10038-1) - UPC - AP - -- - 575.105 -  -  - DIS
CNPAF - (21271-1) - UPC - AP - PP - 1996.009 -  - 1996.009 - DIS