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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
10/01/2012 |
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Data da última atualização: |
10/01/2012 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
FARIA, B. M. S. de; PAPPAS JUNIOR, G. J.; GUIMARÃES, C. M.; CARVALHO, P. F. Z.; VIANELLO, R. P. |
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Afiliação: |
BÁRBARA MÜLLER SALOMÃO DE FARIA, mestranda UFV; GEORGIOS JOANNIS PAPPAS JUNIOR, CENARGEN; CLEBER MORAIS GUIMARAES, CNPAF; PATRÍCIA FERNANDA ZAMBUZZI CARVALHO, UFG; ROSANA PEREIRA VIANELLO BRONDANI, CNPAF. |
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Título: |
Identificação e quantificação da expressão de genes diferencialmente expressos sob condição de déficit hídrico em feijoeiro comum (Phaseolus vulgaris). |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
In: CONGRESSO NACIONAL DE PESQUISA DE FEIJÃO, 10., 2011, Goiânia. Anais... Goiânia: Embrapa Arroz e Feijão, 2011. |
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Descrição Física: |
1 CD-ROM. |
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Idioma: |
Português |
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Notas: |
CONAFE. |
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Conteúdo: |
Com o intuito de avançar no conhecimento dos mecanismos genéticos envolvidos na tolerância à seca, esse estudo teve como objetivos a identificação de genes diferencialmente expressos em feijoeiro comum sob condições de déficit hídrico, validação via RT-PCT de alguns desses genes-candidatos potencialmente envolvidos na resposta ao estresse, bem como contribuir para o incremento do banco de sequências expressas (EST?s) enriquecidas para genes diferencialmente expressos nos genótipos BAT 477 (tolerante) e Pérola (suscetível) quando submetidos às condições de presença e ausência do déficit hídrico. |
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Thesagro: |
Deficiência hídrica; Feijão; Gene; Phaseolus vulgaris. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/51941/1/biotc6.pdf
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Marc: |
LEADER 01414nam a2200229 a 4500
001 1912174
005 2012-01-10
008 2011 bl uuuu u00u1 u #d
100 1 $aFARIA, B. M. S. de
245 $aIdentificação e quantificação da expressão de genes diferencialmente expressos sob condição de déficit hídrico em feijoeiro comum (Phaseolus vulgaris).
260 $aIn: CONGRESSO NACIONAL DE PESQUISA DE FEIJÃO, 10., 2011, Goiânia. Anais... Goiânia: Embrapa Arroz e Feijão$c2011
300 $c1 CD-ROM.
500 $aCONAFE.
520 $aCom o intuito de avançar no conhecimento dos mecanismos genéticos envolvidos na tolerância à seca, esse estudo teve como objetivos a identificação de genes diferencialmente expressos em feijoeiro comum sob condições de déficit hídrico, validação via RT-PCT de alguns desses genes-candidatos potencialmente envolvidos na resposta ao estresse, bem como contribuir para o incremento do banco de sequências expressas (EST?s) enriquecidas para genes diferencialmente expressos nos genótipos BAT 477 (tolerante) e Pérola (suscetível) quando submetidos às condições de presença e ausência do déficit hídrico.
650 $aDeficiência hídrica
650 $aFeijão
650 $aGene
650 $aPhaseolus vulgaris
700 1 $aPAPPAS JUNIOR, G. J.
700 1 $aGUIMARÃES, C. M.
700 1 $aCARVALHO, P. F. Z.
700 1 $aVIANELLO, R. P.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Amazônia Oriental. Para informações adicionais entre em contato com cpatu.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Amazônia Oriental. |
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Data corrente: |
20/04/2021 |
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Data da última atualização: |
20/04/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
CALLEGARI, D. M.; LIMA, A. M.; BARROS, N. L. F.; SIQUEIRA, A. S.; CUNHA, E. F. M.; SOUZA, C. R. B. de. |
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Afiliação: |
DAIHANY MORAES CALLEGARI, UFPA / UFRA; ALINE MEDEIROS LIMA, UFPA / UFRA; NICOLLE LOUISE FERREIRA BARROS, UFPA; ANDREI SANTOS SIQUEIRA, UFPA; ELISA FERREIRA MOURA CUNHA, CPATU; CLAÚDIA REGINA BATISTA DE SOUZA, UFPA. |
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Título: |
Changes in transcript levels of cassava superoxide dismutase and catalase during interaction with Phytopythium sp. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Physiological and Molecular Plant Pathology, v. 114, Apr. 2021. Article 101629. |
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DOI: |
https://doi.org/10.1016/j.pmpp.2021.101629 |
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Idioma: |
Inglês |
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Conteúdo: |
Among the biotic factors affecting the production of cassava (Manihot esculenta Crantz), root rot is one of the most severe diseases worldwide. In northern Brazil, the oomycete Phytopythium sp. causes cassava soft root rot disease in the Amazon region. Seeking to understand this plant-pathogen interaction, our main aim was to evaluate changes in expression levels of superoxide dismutase (SOD) and catalase (CAT) genes in susceptible cassava infected by Phytopythium sp., using semi-quantitative RT-PCR assays. Thus, four SOD (three CuZnSOD and one MnSOD) and three CAT genes were selected from the cassava genome available in the Phytozome Database. Comparative sequence analysis revealed high identity between the deduced MeSOD and MeCAT proteins with SODs and CATs from GenBank Database. In addition, putative conserved motifs and metal-binding domains were found within MeCuZnSOD1-3 and MeMnSOD sequences. The deduced MeCAT proteins contain putative domains and conserved motifs, such as a peroxisomal targeting signal. Furthermore, phylogenetic analysis showed that MeCuZnSOD1 and MeCuZnSOD3 proteins were related to SOD [Cu?Zn] 2 (XP_021686055.1) from Hevea brasiliensis, while MeCuZnSOD2 was close to SOD [Cu?Zn] (XP_012089157.1) from Jatropha curcas. MeMnSOD showed a phylogenetic relationship with mitochondrial SOD [Mn] (XP_021684793.1) from H. brasiliensis. The results also revealed phylogenetic proximity of MeCAT2 and MeCAT3 with CAT isozyme 2 (XP_021675148.1), while MeCAT1 was close to CAT isozyme 1 (XP_021675149.1), both from H. brasiliensis. Semi-quantitative RT-PCR assays showed that Phytopythium sp. was able to suppress the expression of MeSOD genes at early stages of infection, while at 48 h after inoculation (HAI) cassava responded by increasing the expression of the MeCuZnSOD3 gene. For the CAT genes, the MeCAT1 gene showed an expression pattern similar to MeCuZnSOD3, while the expression of the MeCAT3 gene was increased at 24 and 48 HAI. The results revealed that cassava responded to Phytopythium sp. infection through changes in transcript levels of different SOD and CAT isoforms, which are known enzymes with crucial functions regarding the oxidative burst produced during plant-pathogen interactions. MenosAmong the biotic factors affecting the production of cassava (Manihot esculenta Crantz), root rot is one of the most severe diseases worldwide. In northern Brazil, the oomycete Phytopythium sp. causes cassava soft root rot disease in the Amazon region. Seeking to understand this plant-pathogen interaction, our main aim was to evaluate changes in expression levels of superoxide dismutase (SOD) and catalase (CAT) genes in susceptible cassava infected by Phytopythium sp., using semi-quantitative RT-PCR assays. Thus, four SOD (three CuZnSOD and one MnSOD) and three CAT genes were selected from the cassava genome available in the Phytozome Database. Comparative sequence analysis revealed high identity between the deduced MeSOD and MeCAT proteins with SODs and CATs from GenBank Database. In addition, putative conserved motifs and metal-binding domains were found within MeCuZnSOD1-3 and MeMnSOD sequences. The deduced MeCAT proteins contain putative domains and conserved motifs, such as a peroxisomal targeting signal. Furthermore, phylogenetic analysis showed that MeCuZnSOD1 and MeCuZnSOD3 proteins were related to SOD [Cu?Zn] 2 (XP_021686055.1) from Hevea brasiliensis, while MeCuZnSOD2 was close to SOD [Cu?Zn] (XP_012089157.1) from Jatropha curcas. MeMnSOD showed a phylogenetic relationship with mitochondrial SOD [Mn] (XP_021684793.1) from H. brasiliensis. The results also revealed phylogenetic proximity of MeCAT2 and MeCAT3 with CAT isozyme 2 (XP_021675148.1), while MeCAT1 was clos... Mostrar Tudo |
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Palavras-Chave: |
Phytopythium. |
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Thesagro: |
Doença de Planta; Mandioca; Manihot Esculenta; Podridão da Raiz. |
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Thesaurus NAL: |
Cassava; Root rot. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 03112naa a2200277 a 4500
001 2131417
005 2021-04-20
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.pmpp.2021.101629$2DOI
100 1 $aCALLEGARI, D. M.
245 $aChanges in transcript levels of cassava superoxide dismutase and catalase during interaction with Phytopythium sp.$h[electronic resource]
260 $c2021
520 $aAmong the biotic factors affecting the production of cassava (Manihot esculenta Crantz), root rot is one of the most severe diseases worldwide. In northern Brazil, the oomycete Phytopythium sp. causes cassava soft root rot disease in the Amazon region. Seeking to understand this plant-pathogen interaction, our main aim was to evaluate changes in expression levels of superoxide dismutase (SOD) and catalase (CAT) genes in susceptible cassava infected by Phytopythium sp., using semi-quantitative RT-PCR assays. Thus, four SOD (three CuZnSOD and one MnSOD) and three CAT genes were selected from the cassava genome available in the Phytozome Database. Comparative sequence analysis revealed high identity between the deduced MeSOD and MeCAT proteins with SODs and CATs from GenBank Database. In addition, putative conserved motifs and metal-binding domains were found within MeCuZnSOD1-3 and MeMnSOD sequences. The deduced MeCAT proteins contain putative domains and conserved motifs, such as a peroxisomal targeting signal. Furthermore, phylogenetic analysis showed that MeCuZnSOD1 and MeCuZnSOD3 proteins were related to SOD [Cu?Zn] 2 (XP_021686055.1) from Hevea brasiliensis, while MeCuZnSOD2 was close to SOD [Cu?Zn] (XP_012089157.1) from Jatropha curcas. MeMnSOD showed a phylogenetic relationship with mitochondrial SOD [Mn] (XP_021684793.1) from H. brasiliensis. The results also revealed phylogenetic proximity of MeCAT2 and MeCAT3 with CAT isozyme 2 (XP_021675148.1), while MeCAT1 was close to CAT isozyme 1 (XP_021675149.1), both from H. brasiliensis. Semi-quantitative RT-PCR assays showed that Phytopythium sp. was able to suppress the expression of MeSOD genes at early stages of infection, while at 48 h after inoculation (HAI) cassava responded by increasing the expression of the MeCuZnSOD3 gene. For the CAT genes, the MeCAT1 gene showed an expression pattern similar to MeCuZnSOD3, while the expression of the MeCAT3 gene was increased at 24 and 48 HAI. The results revealed that cassava responded to Phytopythium sp. infection through changes in transcript levels of different SOD and CAT isoforms, which are known enzymes with crucial functions regarding the oxidative burst produced during plant-pathogen interactions.
650 $aCassava
650 $aRoot rot
650 $aDoença de Planta
650 $aMandioca
650 $aManihot Esculenta
650 $aPodridão da Raiz
653 $aPhytopythium
700 1 $aLIMA, A. M.
700 1 $aBARROS, N. L. F.
700 1 $aSIQUEIRA, A. S.
700 1 $aCUNHA, E. F. M.
700 1 $aSOUZA, C. R. B. de
773 $tPhysiological and Molecular Plant Pathology$gv. 114, Apr. 2021. Article 101629.
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