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Registro Completo |
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
04/03/1998 |
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Data da última atualização: |
16/06/2017 |
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Autoria: |
MITYKO, J.; FARI, M. |
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Título: |
Problems and results of doubled haploid plant production in pepper (Capsicum annum L.) via anther - and microspore culture. |
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Ano de publicação: |
1997 |
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Fonte/Imprenta: |
Acta Horticulturae, Leuven, n. 447, p.281-287, 1997. |
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Idioma: |
Inglês |
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Notas: |
Edição do Proceedings of the 3 International Symposium on in Vitro Culture and Horticultural Breeding, Jerusalem, Israel, oct. 1996. |
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Conteúdo: |
More than 500 pepper varieties, breeding lines and F1 hybrids were investigated in anther culture. A decreasing androgenic response was obtained in wax-type, dark green blocky-type, tomato-shaped and Cayenne;chilli-type peppers (from 76 to 0 plants/100 cultured anthers). Although bell peppers generated a high? and reproducible response, spice peppers proved to be less or non-responsive. An in vitro colchicine treatment was applied to the haploid regenerated plants for chromosome doubling with a 75% rediplolidization rate. One responsive (Feherozon) and two poorly responsive (No. 40017 R-13 and No. 40017 RK-II) genotypes were selected for microspore culture experiments. Three basic culture media were used with different carbohydrate and growth regulator supplements. the highest frequency of microspore divisions was obtained on NLN-2-15 medium (modified LIchter, 1981 medium containing 2% saccharose, 0,5 mg/l NAA and 0,5 mg/l BA). Sporophytic divisions resulted in some multicellular pollens, but enbryo development from these structures has not been obtained ywt. Stigma co-culture, exine wall degestioni and the colchicine effect on microspore divisions were investigated. Although neither the stigma co-culture nor the enzyme treatment could increase the cell division frequency , the positive effect of the drug (25 mg;l for 24 h) on the microspore development was clear. |
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Palavras-Chave: |
Colchicina in vitro; In vitro colchicine; Production; Treatment. |
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Thesagro: |
Capsicum Annuum; Cultura de Tecido; Melhoramento; Pimentão; Produção; Tratamento. |
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Thesaurus Nal: |
breeding; pepper; tissue culture. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02337naa a2200301 a 4500
001 1132597
005 2017-06-16
008 1997 bl uuuu u00u1 u #d
100 1 $aMITYKO, J.
245 $aProblems and results of doubled haploid plant production in pepper (Capsicum annum L.) via anther - and microspore culture.
260 $c1997
500 $aEdição do Proceedings of the 3 International Symposium on in Vitro Culture and Horticultural Breeding, Jerusalem, Israel, oct. 1996.
520 $aMore than 500 pepper varieties, breeding lines and F1 hybrids were investigated in anther culture. A decreasing androgenic response was obtained in wax-type, dark green blocky-type, tomato-shaped and Cayenne;chilli-type peppers (from 76 to 0 plants/100 cultured anthers). Although bell peppers generated a high? and reproducible response, spice peppers proved to be less or non-responsive. An in vitro colchicine treatment was applied to the haploid regenerated plants for chromosome doubling with a 75% rediplolidization rate. One responsive (Feherozon) and two poorly responsive (No. 40017 R-13 and No. 40017 RK-II) genotypes were selected for microspore culture experiments. Three basic culture media were used with different carbohydrate and growth regulator supplements. the highest frequency of microspore divisions was obtained on NLN-2-15 medium (modified LIchter, 1981 medium containing 2% saccharose, 0,5 mg/l NAA and 0,5 mg/l BA). Sporophytic divisions resulted in some multicellular pollens, but enbryo development from these structures has not been obtained ywt. Stigma co-culture, exine wall degestioni and the colchicine effect on microspore divisions were investigated. Although neither the stigma co-culture nor the enzyme treatment could increase the cell division frequency , the positive effect of the drug (25 mg;l for 24 h) on the microspore development was clear.
650 $abreeding
650 $apepper
650 $atissue culture
650 $aCapsicum Annuum
650 $aCultura de Tecido
650 $aMelhoramento
650 $aPimentão
650 $aProdução
650 $aTratamento
653 $aColchicina in vitro
653 $aIn vitro colchicine
653 $aProduction
653 $aTreatment
700 1 $aFARI, M.
773 $tActa Horticulturae, Leuven$gn. 447, p.281-287, 1997.
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Registro original: |
Embrapa Semiárido (CPATSA) |
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| Registros recuperados : 18 | |
| 11. |  | MELO, N. F. de; TEIXEIRA, J. B.; FARI, M. In vitro cultivation on modal segments of the umbu tree [Spondias tuberosa (Arr.) Cam]. Acta Horticulturae, Leuven, n. 447, p. 535-537, 1997. Edição do Proceedings of the 3 International Symposium on in Vitro Culture and Horticultural Breeding, Jerusalem, Israel, oct. 1996.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - B |
| Biblioteca(s): Embrapa Semiárido. |
|    |
| 13. | | FARI, M.; SORVARI, S.; AHANEN, K; TOLDI, O.; MELO, N. F. de; HIRSALMI, H. Development and germination of somatic embryos on sugar-free wheat starch medium in carrot (Daucus carota L. cv. Nantes Duke). In: ENCONTRO BRASILEIRO DE BIOTECNOLOGIA VEGETAL, 2., 1997, Gramado. Programas e resumos... Passo Fundo: REDBIO Sub-Região Brasil, 1997. p. 128.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
|  |
| 15. | | FARI, M.; SORVARI, S.; AHANEN, K.; MELO, N. F. de; HIRSALMI, H. Production of dry type artificial seeds by means of PVC-FOIL desiccation method from genetically engineered somatic embryos of carrot (Daucus carota L., cv. Nantes Duke). In: ENCONTRO BRASILEIRO DE BIOTECNOLOGIA VEGETAL, 2., 1997, Gramado. Programas e resumos... Passo Fundo: REDBIO Sub-Região Brasil, 1997. p. 129.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
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| Registros recuperados : 18 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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