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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
25/08/2020 |
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Data da última atualização: |
25/08/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ARENA, G. D.; RAMOS-GONZALEZ, P. L.; FALK, B. W.; CASTEEL, C. L.; ASTUA, J. de F.; MACHADO, M. A. |
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Afiliação: |
GABRIELLA D. ARENA, Centro de Citricultura Sylvio Moreira; PEDRO LUIS RAMOS-GONZALEZ, Instituto Biológico; BRYCE W. FALK, University of California; CLARE L. CASTEEL, CASTEEL; JULIANA DE FREITAS ASTUA, CNPMF; MARCOS A. MACHADO, Centro de Citricultura Sylvio Moreira. |
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Título: |
Plant immune system activation upon Citrus Leprosis Virus c infection is mimicked by the ectopic expression of the P61 viral protein. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Frontiers in Plant Science, August, 2020. |
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Idioma: |
Inglês |
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Conteúdo: |
Citrus leprosis virus C (CiLV-C, genus Cilevirus, family Kitaviridae) is an atypical virus that does not spread systemically in its plant hosts. Upon its inoculation by Brevipalpus mites, only localized lesions occur, and the infection remains limited to cells around mite feeding sites. Here, we aimed to gain insights into the putative causes of viral unfitness in plants by expanding the limited knowledge of the molecular mechanisms underlying plant/kitavirid interactions. Firstly, we quantified the CiLV-C viral RNAs during the infection in Arabidopsis thaliana plants using RT-qPCR and systematized it by defining three stages of distinguishing subgenomic and genomic RNA accumulation: i) 0–24 h after infestation, ii) 2–4 days after infestation (dai), and iii) 6–10 dai. Accordingly, the global plant response to CiLV-C infection was assessed by RNA-Seq at each period. Results indicated a progressive reprogramming of the plant transcriptome in parallel to the increasing viral loads. Gene ontology enrichment analysis revealed the induction of cell growth-related processes at the early stages of the infection and the triggering of the SA-mediated pathway, ROS burst and hypersensitive response (HR) at the presymptomatic stage. Conversely, infected plants downregulated JA/ET-mediated pathways and processes involved in the primary metabolism including photosynthesis. Marker genes of unfolded protein response were also induced, suggesting a contribution of the endoplasmic reticulum stress to the cell death caused by the viral infection. Finally, we transiently expressed CiLV-C proteins in Nicotiana benthamiana plants to undertake their roles in the elicited plant responses. Expression of the CiLV-C P61 protein consistently triggered ROS burst, upregulated SA- and HR-related genes, increased SA levels, reduced JA levels, and caused cell death. Mimicry of responses typically observed during CiLV-C–plant interaction indicates P61 as a putative viral effector causing the HR-like symptoms associated with the infection. Our data strengthen the hypothesis that symptoms of CiLV-C infection might be the outcome of a hypersensitive-like response during an incompatible interaction. Consequently, the locally restricted infection of CiLV-C, commonly observed across infections by kitavirids, supports the thesis that these viruses, likely arising from an ancestral arthropod-infecting virus, are unable to fully circumvent plant defenses. MenosCitrus leprosis virus C (CiLV-C, genus Cilevirus, family Kitaviridae) is an atypical virus that does not spread systemically in its plant hosts. Upon its inoculation by Brevipalpus mites, only localized lesions occur, and the infection remains limited to cells around mite feeding sites. Here, we aimed to gain insights into the putative causes of viral unfitness in plants by expanding the limited knowledge of the molecular mechanisms underlying plant/kitavirid interactions. Firstly, we quantified the CiLV-C viral RNAs during the infection in Arabidopsis thaliana plants using RT-qPCR and systematized it by defining three stages of distinguishing subgenomic and genomic RNA accumulation: i) 0–24 h after infestation, ii) 2–4 days after infestation (dai), and iii) 6–10 dai. Accordingly, the global plant response to CiLV-C infection was assessed by RNA-Seq at each period. Results indicated a progressive reprogramming of the plant transcriptome in parallel to the increasing viral loads. Gene ontology enrichment analysis revealed the induction of cell growth-related processes at the early stages of the infection and the triggering of the SA-mediated pathway, ROS burst and hypersensitive response (HR) at the presymptomatic stage. Conversely, infected plants downregulated JA/ET-mediated pathways and processes involved in the primary metabolism including photosynthesis. Marker genes of unfolded protein response were also induced, suggesting a contribution of the endoplasmic reticulum st... Mostrar Tudo |
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Thesagro: |
Fruta Cítrica. |
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Categoria do assunto: |
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Marc: |
LEADER 03074naa a2200193 a 4500
001 2124551
005 2020-08-25
008 2020 bl uuuu u00u1 u #d
100 1 $aARENA, G. D.
245 $aPlant immune system activation upon Citrus Leprosis Virus c infection is mimicked by the ectopic expression of the P61 viral protein.$h[electronic resource]
260 $c2020
520 $aCitrus leprosis virus C (CiLV-C, genus Cilevirus, family Kitaviridae) is an atypical virus that does not spread systemically in its plant hosts. Upon its inoculation by Brevipalpus mites, only localized lesions occur, and the infection remains limited to cells around mite feeding sites. Here, we aimed to gain insights into the putative causes of viral unfitness in plants by expanding the limited knowledge of the molecular mechanisms underlying plant/kitavirid interactions. Firstly, we quantified the CiLV-C viral RNAs during the infection in Arabidopsis thaliana plants using RT-qPCR and systematized it by defining three stages of distinguishing subgenomic and genomic RNA accumulation: i) 0–24 h after infestation, ii) 2–4 days after infestation (dai), and iii) 6–10 dai. Accordingly, the global plant response to CiLV-C infection was assessed by RNA-Seq at each period. Results indicated a progressive reprogramming of the plant transcriptome in parallel to the increasing viral loads. Gene ontology enrichment analysis revealed the induction of cell growth-related processes at the early stages of the infection and the triggering of the SA-mediated pathway, ROS burst and hypersensitive response (HR) at the presymptomatic stage. Conversely, infected plants downregulated JA/ET-mediated pathways and processes involved in the primary metabolism including photosynthesis. Marker genes of unfolded protein response were also induced, suggesting a contribution of the endoplasmic reticulum stress to the cell death caused by the viral infection. Finally, we transiently expressed CiLV-C proteins in Nicotiana benthamiana plants to undertake their roles in the elicited plant responses. Expression of the CiLV-C P61 protein consistently triggered ROS burst, upregulated SA- and HR-related genes, increased SA levels, reduced JA levels, and caused cell death. Mimicry of responses typically observed during CiLV-C–plant interaction indicates P61 as a putative viral effector causing the HR-like symptoms associated with the infection. Our data strengthen the hypothesis that symptoms of CiLV-C infection might be the outcome of a hypersensitive-like response during an incompatible interaction. Consequently, the locally restricted infection of CiLV-C, commonly observed across infections by kitavirids, supports the thesis that these viruses, likely arising from an ancestral arthropod-infecting virus, are unable to fully circumvent plant defenses.
650 $aFruta Cítrica
700 1 $aRAMOS-GONZALEZ, P. L.
700 1 $aFALK, B. W.
700 1 $aCASTEEL, C. L.
700 1 $aASTUA, J. de F.
700 1 $aMACHADO, M. A.
773 $tFrontiers in Plant Science, August, 2020.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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Registro Completo
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
14/01/2011 |
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Data da última atualização: |
14/01/2011 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 4 |
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Autoria: |
MATTOS, P. P. de; OLIVEIRA, M. F.; AGUSTINI, A. F.; BRAZ, E. M.; RIVERA, H.; OLIVEIRA, Y. M. M. de; ROSOT, M. A. D.; GARRASTAZU, M. C. |
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Afiliação: |
PATRICIA POVOA DE MATTOS, CNPF; Mariana Ferraz Oliveira, UFPR; Andrea Fernanda Agustini, UFPR; EVALDO MUNOZ BRAZ, CNPF; Hugo Rivera, Conaf-Chile; YEDA MARIA MALHEIROS DE OLIVEIRA, CNPF; MARIA AUGUSTA DOETZER ROSOT, CNPF; MARILICE CORDEIRO GARRASTAZU, CNPF. |
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Título: |
Aceleração do crescimento em diâmetro de espécies da Floresta Ombrófila Mista nos últimos 90 anos. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Pesquisa Florestal Brasileira, Colombo, v. 30, n. 64, p. 319-326, nov./dez. 2010. |
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DOI: |
10.4336/2010.pfb.30.64.319 |
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Idioma: |
Português |
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Conteúdo: |
A Floresta Ombrófila Mista é considerada uma das principais formações do bioma Mata Atlântica. Apesar de sua importância, existe uma carência de informações sobre a dinâmica de crescimento de espécies dessa tipologia florestal. O presente estudo foi desenvolvido com o objetivo de estimar o crescimento passado de espécies arbóreas de Floresta Ombrófila Mista. Foram realizadas avaliações dendrocronológicas não destrutivas nas espécies Araucaria angustifolia, Ocotea porosa, Ilex paraguariensis, Cedrela fissilis, Ocotea puberula e Ocotea pulchella. O crescimento periódico médio em diâmetro até 2006 foi de aproximadamente 0,5 cm ano-1, para A. angustifolia, C. fissilis, O. porosa e O. pulchella. O. puberula e I. paraguariensis apresentaram crescimento periódico médio em diâmetro até 2006 igual ou superior a 0,9 cm ano-1. Foi observado uma aceleração do crescimento médio a partir das primeiras décadas do século passado. Sugere-se que esse padrão de crescimento seja consequência de variações climáticas locais ao longo desse período. |
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Palavras-Chave: |
Crescimento diamétrico; Dendocronologia. |
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Thesagro: |
Araucária Angustifólia; Floresta Nativa. |
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Categoria do assunto: |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/25495/1/102-831-3-PB.pdf
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Marc: |
LEADER 01930naa a2200265 a 4500
001 1873155
005 2011-01-14
008 2010 bl uuuu u00u1 u #d
024 7 $a10.4336/2010.pfb.30.64.319$2DOI
100 1 $aMATTOS, P. P. de
245 $aAceleração do crescimento em diâmetro de espécies da Floresta Ombrófila Mista nos últimos 90 anos.$h[electronic resource]
260 $c2010
520 $aA Floresta Ombrófila Mista é considerada uma das principais formações do bioma Mata Atlântica. Apesar de sua importância, existe uma carência de informações sobre a dinâmica de crescimento de espécies dessa tipologia florestal. O presente estudo foi desenvolvido com o objetivo de estimar o crescimento passado de espécies arbóreas de Floresta Ombrófila Mista. Foram realizadas avaliações dendrocronológicas não destrutivas nas espécies Araucaria angustifolia, Ocotea porosa, Ilex paraguariensis, Cedrela fissilis, Ocotea puberula e Ocotea pulchella. O crescimento periódico médio em diâmetro até 2006 foi de aproximadamente 0,5 cm ano-1, para A. angustifolia, C. fissilis, O. porosa e O. pulchella. O. puberula e I. paraguariensis apresentaram crescimento periódico médio em diâmetro até 2006 igual ou superior a 0,9 cm ano-1. Foi observado uma aceleração do crescimento médio a partir das primeiras décadas do século passado. Sugere-se que esse padrão de crescimento seja consequência de variações climáticas locais ao longo desse período.
650 $aAraucária Angustifólia
650 $aFloresta Nativa
653 $aCrescimento diamétrico
653 $aDendocronologia
700 1 $aOLIVEIRA, M. F.
700 1 $aAGUSTINI, A. F.
700 1 $aBRAZ, E. M.
700 1 $aRIVERA, H.
700 1 $aOLIVEIRA, Y. M. M. de
700 1 $aROSOT, M. A. D.
700 1 $aGARRASTAZU, M. C.
773 $tPesquisa Florestal Brasileira, Colombo$gv. 30, n. 64, p. 319-326, nov./dez. 2010.
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Embrapa Florestas (CNPF) |
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