Data/hora: 01/06/2024 10:10:45

Biblioteca(s): | Embrapa Agroenergia; Embrapa Milho e Sorgo; Embrapa Unidades Centrais.
Data corrente: | 09/02/2018
Data da última atualização: | 18/02/2019
Autoria: | SIHLER, W.; SOUZA, M. L. de; VALICENTE, F. H.; FALCAO, R.; SANCHES, M. M.
Afiliação: | WILLIAM SIHLER, Cenargen; MARLINDA LOBO DE SOUZA, Cenargen; FERNANDO HERCOS VALICENTE, CNPMS; ROSANA FALCAO, CNPAE; MARCIO MARTINELLO SANCHES, Cenargen.
Título: | In vitro infectivity of Spodoptera frugiperda multiple nucleopolyhedrovirus to different insect cell lines.
Ano de publicação: | 2018
Fonte/Imprenta: | Pesquisa Agropecuária Brasileira, Brasília, v. 53, n. 1, p. 1-9, 2018.
DOI: | 10.1590/S0100-204X2018000100001
Idioma: | Inglês
Conteúdo: | The objective of this work was to evaluate the response of an in vitro host range to Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV), a pathogenic virus to the fall armyworm (Spodoptera frugiperda, Lepidoptera: Noctuidae), for the further development of a biopesticide based on cell culture systems. The cell lines from Bombyx mori (BM-5), Lymantria dispar (IPLB-LD-625Y), Trichoplusia ni (BTITn-5B1-4), Anticarsia gemmatalis (UFL-AG-286), and S. frugiperda (IPLB-SF-21AE and Sf9) were tested for their susceptibility to a highly-virulent Brazilian isolate of SfMNPV. The cytopathic effects induced by the virus, the production of viral particles, and the synthesis of viral polypeptides were examined and compared. Both S. frugiperda cell lines showed hypertrophy of cell nuclei and production of many polyhedra. The SDSPage of radiolabed proteins showed that the cell protein synthesis was shutoff, while an intense band of about 30 kDa, recognized as polyhedrin, was synthesized. The other cell lines did not show polyhedra production, although some of them underwent morphological changes and protein synthesis shutdown in response to virus infection. The SF-21 and Sf9 cell lines are recommended for further in vitro production of SfMNPV.
Palavras-Chave: | Cultura de células; Lagarta-do-cartucho; Produção in vitro; SfMNPV.
Thesagro: | Baculovirus; Controle biológico; Spodoptera Frugiperda.
Thesaurus Nal: | Baculoviridae; Biological control; Cell culture; Spodoptera frugiperda multiple nucleopolyhedrovirus.
Categoria do assunto: | --

URL:

http://ainfo.cnptia.embrapa.br/digital/bitstream/item/172484/1/53n01a01.pdf
http://ainfo.cnptia.embrapa.br/digital/bitstream/item/173947/1/In-vitro-infectivity-of-Spodoptera-frugiperda.pdf

Registro original: | Embrapa Milho e Sorgo (CNPMS)

Exemplares:

CNPAE - (3289-1) - UPC - AP - DD -  -  -  - DIS
AI-SEDE - (62274-1) - UPE - AP - DD -  -  -  - DIS
CNPMS - (28191-1) - UPC - AP - DD -  -  -  - DIS