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Registro Completo |
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Biblioteca(s): |
Embrapa Uva e Vinho. |
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Data corrente: |
27/08/2014 |
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Data da última atualização: |
02/04/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FALAVIGNA, V. da S.; PORTO, D. D.; BUFFON, V.; MARGIS-PINHEIRO, M.; PASQUALI, G.; REVERS, L. F. |
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Afiliação: |
Vítor da Silveira Falavigna; Diogo Denardi Porto, CNPUV; Vanessa Buffon, CNPUV; Márcia Margis-Pinheiro; Giancarlo Pasquali; LUIS FERNANDO REVERS, CNPUV. |
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Título: |
Differential transcriptional profiles of dormancy-related genes in apple buds |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Plant Molecular Biology Reporter, v. 32, p. 796-813, 2014. |
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Idioma: |
Inglês |
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Notas: |
DOI 10.1007/s11105-013-0690-0 |
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Conteúdo: |
The production of temperate fruit crops depends on plant developmental processes, primarily the shift from the juvenile phase to the reproductive phase, dormancy transitions and flowering. Apple tree (Malus ×domestica Borkh.) development is regulated by chilling temperatures, which are required for bud dormancy progression. The apple cultivar Castel Gala is a spontaneous mutation of "Gala Standard". "Castel Gala" is characterized by a 50 % decrease in the chilling requirement (CR) for dormancy release, which results in an earlier budbreak. This work explores the contrasting phenotypes of these cultivars using suppression subtractive hybridization (SSH). From 1,019 unigenes identified by SSH, we selected 28 candidate genes putatively associated with dormancy cycling. Reverse transcription-quantitative polymerase chain reaction was used to validate the differential expression profiles and to transcriptionally characterize these genes in three distinct apple cultivars ("Castel Gala", "Royal Gala" and "Fuji Standard") during a cycle comprising growth to dormancy. Of the 28 candidate genes analyzed, 17 confirmed the differences in expression predicted by SSH. Seasonal transcript accumulation during the winter was observed for several genes, with higher steady-state mRNA levels maintained longer in cultivars with a high CR. The transcription profiles suggest that these genes may be associated with dormancy establishment and maintenance. Of the 17 candidate genes, transcripts coding for dormancy-associated MADS-box (DAM), dehydrins, GAST1, LTI65, NAC, HTA8, HTA12 and RAP2.12-like proteins displayed major differences in gene expression between cultivars through the winter. These genes were therefore considered good candidates for key roles in the dormancy process in apple trees. MenosThe production of temperate fruit crops depends on plant developmental processes, primarily the shift from the juvenile phase to the reproductive phase, dormancy transitions and flowering. Apple tree (Malus ×domestica Borkh.) development is regulated by chilling temperatures, which are required for bud dormancy progression. The apple cultivar Castel Gala is a spontaneous mutation of "Gala Standard". "Castel Gala" is characterized by a 50 % decrease in the chilling requirement (CR) for dormancy release, which results in an earlier budbreak. This work explores the contrasting phenotypes of these cultivars using suppression subtractive hybridization (SSH). From 1,019 unigenes identified by SSH, we selected 28 candidate genes putatively associated with dormancy cycling. Reverse transcription-quantitative polymerase chain reaction was used to validate the differential expression profiles and to transcriptionally characterize these genes in three distinct apple cultivars ("Castel Gala", "Royal Gala" and "Fuji Standard") during a cycle comprising growth to dormancy. Of the 28 candidate genes analyzed, 17 confirmed the differences in expression predicted by SSH. Seasonal transcript accumulation during the winter was observed for several genes, with higher steady-state mRNA levels maintained longer in cultivars with a high CR. The transcription profiles suggest that these genes may be associated with dormancy establishment and maintenance. Of the 17 candidate genes, transcripts codin... Mostrar Tudo |
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Palavras-Chave: |
Apple; Castel Gala; Fuji Standard; Royal Gala. |
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Thesagro: |
Dormencia; Genetica vegetal; Maçã; Temperatura. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/107373/1/Falavigna2014-Genes-Apple-Buds.pdf
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Marc: |
LEADER 02604naa a2200289 a 4500
001 1993551
005 2019-04-02
008 2014 bl uuuu u00u1 u #d
100 1 $aFALAVIGNA, V. da S.
245 $aDifferential transcriptional profiles of dormancy-related genes in apple buds$h[electronic resource]
260 $c2014
500 $aDOI 10.1007/s11105-013-0690-0
520 $aThe production of temperate fruit crops depends on plant developmental processes, primarily the shift from the juvenile phase to the reproductive phase, dormancy transitions and flowering. Apple tree (Malus ×domestica Borkh.) development is regulated by chilling temperatures, which are required for bud dormancy progression. The apple cultivar Castel Gala is a spontaneous mutation of "Gala Standard". "Castel Gala" is characterized by a 50 % decrease in the chilling requirement (CR) for dormancy release, which results in an earlier budbreak. This work explores the contrasting phenotypes of these cultivars using suppression subtractive hybridization (SSH). From 1,019 unigenes identified by SSH, we selected 28 candidate genes putatively associated with dormancy cycling. Reverse transcription-quantitative polymerase chain reaction was used to validate the differential expression profiles and to transcriptionally characterize these genes in three distinct apple cultivars ("Castel Gala", "Royal Gala" and "Fuji Standard") during a cycle comprising growth to dormancy. Of the 28 candidate genes analyzed, 17 confirmed the differences in expression predicted by SSH. Seasonal transcript accumulation during the winter was observed for several genes, with higher steady-state mRNA levels maintained longer in cultivars with a high CR. The transcription profiles suggest that these genes may be associated with dormancy establishment and maintenance. Of the 17 candidate genes, transcripts coding for dormancy-associated MADS-box (DAM), dehydrins, GAST1, LTI65, NAC, HTA8, HTA12 and RAP2.12-like proteins displayed major differences in gene expression between cultivars through the winter. These genes were therefore considered good candidates for key roles in the dormancy process in apple trees.
650 $aDormencia
650 $aGenetica vegetal
650 $aMaçã
650 $aTemperatura
653 $aApple
653 $aCastel Gala
653 $aFuji Standard
653 $aRoyal Gala
700 1 $aPORTO, D. D.
700 1 $aBUFFON, V.
700 1 $aMARGIS-PINHEIRO, M.
700 1 $aPASQUALI, G.
700 1 $aREVERS, L. F.
773 $tPlant Molecular Biology Reporter$gv. 32, p. 796-813, 2014.
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Registro original: |
Embrapa Uva e Vinho (CNPUV) |
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Registro Completo
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
18/05/2004 |
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Data da última atualização: |
04/08/2022 |
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Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
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Autoria: |
SABOURIN, E.; CARON, P. |
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Afiliação: |
ERIC SABOURIN, Consultor CIRAD/CPATSA; PATRICK CARON, Consultor CIRAD/CPATSA. |
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Título: |
Origem e evolução da agricultura familiar no Nordeste semi-árido. |
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Ano de publicação: |
2003 |
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Fonte/Imprenta: |
In: CARON, P.; SABOURIN, E. (Ed.). Camponeses do Sertão: mutação das agriculturas familiares no Nordeste do Brasil. Brasília, DF: Embrapa Informação Tecnológica; CIRAD, 2003. |
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Páginas: |
p. 29-45. |
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ISBN: |
85-7383-222-3 |
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Idioma: |
Português |
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Conteúdo: |
O desenvolvimento agrário no Nordeste Semi-árido; Agricultura familiar: uma história de resistência e adaptações; A colonização do sertão; O recuo econômico e o surgimento dos camponeses; As frentes pioneiras, a apropriação do espaço e a modernização agrícola; Um interesse renovado pela agricultura familiar brasileira. |
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Palavras-Chave: |
Evolução econômica; Nordeste; Origem da agricultura familiar; Produtor rural; Semi-árido. |
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Thesagro: |
Agricultura de Subsistência; Agricultura familiar; Desenvolvimento rural. |
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Thesaurus NAL: |
Farming systems; Rural development. |
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Categoria do assunto: |
B Sociologia Rural |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/196557/1/Camponeses-do-Sertao-pag-29-45.pdf
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Marc: |
LEADER 01245naa a2200277 a 4500
001 1153315
005 2022-08-04
008 2003 bl uuuu u00u1 u #d
020 $a85-7383-222-3
100 1 $aSABOURIN, E.
245 $aOrigem e evolução da agricultura familiar no Nordeste semi-árido.
260 $c2003
300 $ap. 29-45.
520 $aO desenvolvimento agrário no Nordeste Semi-árido; Agricultura familiar: uma história de resistência e adaptações; A colonização do sertão; O recuo econômico e o surgimento dos camponeses; As frentes pioneiras, a apropriação do espaço e a modernização agrícola; Um interesse renovado pela agricultura familiar brasileira.
650 $aFarming systems
650 $aRural development
650 $aAgricultura de Subsistência
650 $aAgricultura familiar
650 $aDesenvolvimento rural
653 $aEvolução econômica
653 $aNordeste
653 $aOrigem da agricultura familiar
653 $aProdutor rural
653 $aSemi-árido
700 1 $aCARON, P.
773 $tIn: CARON, P.; SABOURIN, E. (Ed.). Camponeses do Sertão: mutação das agriculturas familiares no Nordeste do Brasil. Brasília, DF: Embrapa Informação Tecnológica; CIRAD, 2003.
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Embrapa Semiárido (CPATSA) |
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