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Registro Completo |
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Biblioteca(s): |
Embrapa Uva e Vinho. |
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Data corrente: |
08/06/2018 |
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Data da última atualização: |
29/04/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BOSCO, D. D.; SINSKI, I.; RITSCHEL, P. S.; CAMARGO, U. A.; FAJARDO, T. V. M.; HARAKAVA, R.; QUECINI, V. |
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Afiliação: |
DANIELA DAL BOSCO, CNPUV; IRACI SINSKI, CNPUV; PATRICIA SILVA RITSCHEL, CNPUV; Umberto A. Camargo; THOR VINICIUS MARTINS FAJARDO, CNPUV; Ricardo Harakava; VERA MARIA QUECINI, CNPUV. |
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Título: |
Expression of disease resistance in genetically modified grapevines correlates with the contents of viral sequences in the T-DNA and global genome methylation. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Transgenic Research, p. 1-18, 2018. [Online] |
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DOI: |
https://doi.org/10.1007/s11248-018-0082-1 |
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Idioma: |
Português |
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Conteúdo: |
Increased tolerance to pathogens is an important goal in conventional and biotechnology-assisted grapevine breeding programs worldwide. Fungal and viral pathogens cause direct losses in berry production, but also affect the quality of the final products. Precision breeding strategies allow the introduction of resistance characters in elite cultivars, although the factors determining the plant?s overall performance are not fully characterized. Grapevine plants expressing defense proteins, from fungal or plant origins, or of the coat protein gene of grapevine leafroll-associated virus 3 (GLRaV-3) were generated by Agrobacterium -mediated transformation of somatic embryos and shoot apical meristems. The responses of the transformed lines to pathogen challenges were investigated by biochemical, phytopathological and molecular methods. The expression of a Metarhizium anisopliae chitinase gene delayed pathogenesis and disease progression against the necrotrophic pathogen Botrytis cinerea. Modified lines expressing a Solanum nigrum osmotin-like protein also exhibited slower disease progression, but to a smaller extent. Grapevine lines carrying two hairpin- inducing constructs had lower GLRaV-3 titers when challenged by grafting, although disease symptoms and viral multiplication were detected. The levels of global genome methylation were determined for the genetically engineered lines, and correlation analyses demonstrated the association between higher levels of methylated DNA and larger portions of virus-derived sequences. Resistance expression was also negatively correlated with the contents of introduced viral sequences and genome methylation, indicating that the effectiveness of resistance strategies employing sequences of viral origin is subject to epigenetic regulation in grapevine. Keywords Chitinase, Epigenetics, Fungus, Grapevine leafroll-associated virus 3, Pathogenesis related protein 5, Vitis MenosIncreased tolerance to pathogens is an important goal in conventional and biotechnology-assisted grapevine breeding programs worldwide. Fungal and viral pathogens cause direct losses in berry production, but also affect the quality of the final products. Precision breeding strategies allow the introduction of resistance characters in elite cultivars, although the factors determining the plant?s overall performance are not fully characterized. Grapevine plants expressing defense proteins, from fungal or plant origins, or of the coat protein gene of grapevine leafroll-associated virus 3 (GLRaV-3) were generated by Agrobacterium -mediated transformation of somatic embryos and shoot apical meristems. The responses of the transformed lines to pathogen challenges were investigated by biochemical, phytopathological and molecular methods. The expression of a Metarhizium anisopliae chitinase gene delayed pathogenesis and disease progression against the necrotrophic pathogen Botrytis cinerea. Modified lines expressing a Solanum nigrum osmotin-like protein also exhibited slower disease progression, but to a smaller extent. Grapevine lines carrying two hairpin- inducing constructs had lower GLRaV-3 titers when challenged by grafting, although disease symptoms and viral multiplication were detected. The levels of global genome methylation were determined for the genetically engineered lines, and correlation analyses demonstrated the association between higher levels of methylated DNA and... Mostrar Tudo |
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Palavras-Chave: |
Fungus; Pathogenesis related protein 5. |
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Thesaurus Nal: |
Chitinase; Epigenetics; Grapevine leafroll-associated virus 3; Vitis. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/178409/1/s11248-018-0082-1-Artigo-v-publicada-Metilacao-DNA-Transgenic-Research-06-06-18.pdf
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Marc: |
LEADER 02824naa a2200277 a 4500
001 2092380
005 2019-04-29
008 2018 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s11248-018-0082-1$2DOI
100 1 $aBOSCO, D. D.
245 $aExpression of disease resistance in genetically modified grapevines correlates with the contents of viral sequences in the T-DNA and global genome methylation.$h[electronic resource]
260 $c2018
520 $aIncreased tolerance to pathogens is an important goal in conventional and biotechnology-assisted grapevine breeding programs worldwide. Fungal and viral pathogens cause direct losses in berry production, but also affect the quality of the final products. Precision breeding strategies allow the introduction of resistance characters in elite cultivars, although the factors determining the plant?s overall performance are not fully characterized. Grapevine plants expressing defense proteins, from fungal or plant origins, or of the coat protein gene of grapevine leafroll-associated virus 3 (GLRaV-3) were generated by Agrobacterium -mediated transformation of somatic embryos and shoot apical meristems. The responses of the transformed lines to pathogen challenges were investigated by biochemical, phytopathological and molecular methods. The expression of a Metarhizium anisopliae chitinase gene delayed pathogenesis and disease progression against the necrotrophic pathogen Botrytis cinerea. Modified lines expressing a Solanum nigrum osmotin-like protein also exhibited slower disease progression, but to a smaller extent. Grapevine lines carrying two hairpin- inducing constructs had lower GLRaV-3 titers when challenged by grafting, although disease symptoms and viral multiplication were detected. The levels of global genome methylation were determined for the genetically engineered lines, and correlation analyses demonstrated the association between higher levels of methylated DNA and larger portions of virus-derived sequences. Resistance expression was also negatively correlated with the contents of introduced viral sequences and genome methylation, indicating that the effectiveness of resistance strategies employing sequences of viral origin is subject to epigenetic regulation in grapevine. Keywords Chitinase, Epigenetics, Fungus, Grapevine leafroll-associated virus 3, Pathogenesis related protein 5, Vitis
650 $aChitinase
650 $aEpigenetics
650 $aGrapevine leafroll-associated virus 3
650 $aVitis
653 $aFungus
653 $aPathogenesis related protein 5
700 1 $aSINSKI, I.
700 1 $aRITSCHEL, P. S.
700 1 $aCAMARGO, U. A.
700 1 $aFAJARDO, T. V. M.
700 1 $aHARAKAVA, R.
700 1 $aQUECINI, V.
773 $tTransgenic Research, p. 1-18, 2018. [Online]
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Registro original: |
Embrapa Uva e Vinho (CNPUV) |
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Biblioteca |
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| Registros recuperados : 283 | |
| 201. |  | TANABE, C. M. N.; FAJARDO, T. V. M.; BUSO, J. A.; AVILA, A. C.; TORRES, A. C.; RESENDE, R. O. A degenerescencia em campo causada por virus na cultura do alho. Fitopatologia Brasileira, Brasilia, v.24, p.362, ago. 1999. Resumo. Suplemento.| Biblioteca(s): Embrapa Hortaliças. |
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| 202. | | SILVA, J. M. F.; PEREIRA, B. R. de M.; AI RWAHNÍH, M.; BLAWID, R.; NAGATA, T.; FAJARDO, T. V. M. Discovery and partial characterization of a novel virus, tentatively named Grapevine virgalike virus. In:Congress of the International Council for the Study of Virus and Virus-like Diseases of the Grapevine (ICVG), 19., 2018, Santiago, Chile. Anais... santiago, Chile: ICGV, April, 2018. p. 146-147.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 203. | | NASCIMENTO, M. B.; FAJARDO, T. V. M.; EIRAS, M.; CZERMAINSKI, A. B. C.; NICKEL, O.; PIO-RIBEIRO, G. Desempenho agronômico de videiras com e sem sintomas de viroses, e comparação molecular de isolados virais. Pesquisa Agropecuária Brasileira, Brasília, DF, V. 50, n. 7, p. 541-550, jul. 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Unidades Centrais; Embrapa Uva e Vinho. |
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| 204. | | VARELA, A. P. M.; RADAELLI, P.; FAJARDO, T. V. M.; EIRAS, M.; KUHN, G. B.; NICKEL, O. Detecção e caracterização molecular do gene da proteína capsidial do Grapevine fanleaf virus, isolado RS. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA UVA E VINHO, 5., ENCONTRO DE PÓS-GRADUANDOS DA EMBRAPA UVA E VINHO, 1., 2007, Bento Gonçalves. Resumos. Bento Gonçalves: Embrapa Uva e Vinho, 2007. p. 26. (Embrapa Uva e Vinho. Documentos, 63). Resumo.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 205. | | RADAELLI, P.; VARELA, A. P. M.; FAJARDO, T. V. M.; EIRAS, M.; KUHN, G. B.; NICKEL, O. Detecção e caracterização molecular do gene da proteína capsidial do Grapevine leafroll-associated virus 2. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA UVA E VINHO, 5., ENCONTRO DE PÓS-GRADUANDOS DA EMBRAPA UVA E VINHO, 1., 2007, Bento Gonçalves. Resumos. Bento Gonçalves: Embrapa Uva e Vinho, 2007. p. 50. (Embrapa Uva e Vinho. Documentos, 63). Resumo.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 206. | | AFONSO, A. O. S.; FARIA, J. L. C.; FAJARDO, T. V. M.; BOTTON, M.; NICKEL, O.; SCHENATO, P. G. Detecção de GLRaV-1 e 3 e GVA em Pseudococcus viburni (Hemiptera: Pseudococcidae) associado à cultura da videira por DAS-ELISA. In.: CONGRESSO BRASILEIRO DE ENTOMOLOGIA , 20., 2004, Gramado, RS. Anais... Gramado: CBE, EN-1006-A, 2004.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 207. | | DUBIELA, C. R.; FAJARDO, T. V. M.; EIRAS, M.; REVERS, L. F.; SOUTO, E. R. de; NICKEL, O. Detecção por RT-PCR em Tempo Real e caracterização molecular do Grapevine virus D e Grapevine leafroll-associated virus 5 em videira. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA, 9.; ENCONTRO DE PÓS-GRADUANDOS DA EMBRAPA UVA E VINHO, 5., 2011, Bento Gonçalves. Resumos... Bento Gonçalves: Embrapa Uva e Vinho, 2011. p. 27. Resumo.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 208. | | GIACOMINI, R.; NICKEL, O.; QUECINI, V.; SINSKI, I.; DAL BOSCO, D.; FAJARDO, T. V. M.; MONTIPÓ, S.; SOUZA, F. de. Efeito de ribavirina sobre a eficácia do cultivo de meristemas na remoção de infecções virais in vitro de macieira. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA UVA E VINHO, 7.; ENCONTRO DE PÓS-GRADUANDOS DA EMBRAPA UVA E VINHO, 3., 2009, Bento Gonçalves. Resumos... Bento Gonçalves: Embrapa Uva e Vinho, 2009. p. 48.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 210. | | FAJARDO, T. V. M.; NISHIJIMA, M.; BUSO, J. A.; TORRES, A. C.; AVILA, A. C.; RESENDE, R. O. Garlic viral complex: identification of Potyviruses and Carlavirus in central Brazil. Fitopatologia Brasileira, Brasilia, v.26, n.3, p.619-626, set. 2001.| Biblioteca(s): Embrapa Hortaliças. |
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| 211. | | GROHS, D. S.; SANTOS, H. P. dos; GARRIDO, L. da R.; FAJARDO, T. V. M.; BOTTON, M. Limpeza viral. CULTIVAR HF, Pelotas, v. 13, n. 93, p. 24-27, ago. /set. 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 5 |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 212. | | BASSO, M. F.; FAJARDO, T. V. M.; SANTOS, H. P. dos; GUERRA, C. C.; AYUB, R. A.; NICKEL, O. Fisiologia foliar e qualidade enológica da uva em videiras infectadas por vírus. Tropical Plant Pathology, Brasília, v. 35, n. 6, p. 351-359, nov./dez. 2010.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 214. | | REZENDE, R. R.; LIMA, T. T. M.; MENDES, T. A. O.; FAJARDO, T. V. M.; ALFENAS-ZERBINI, P. Exploring virus diversity in grapevines. In: CONGRESSO BRASILEIRO DE VIROLOGIA, 29.; ENCONTRO DE VIROLOGIA DO MERCOSUL, 13., 2018, Gramado. Resumos... Brasília: SBV, 2018. p. 321.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 215. | | ALVES, S. A. M.; BONETI, J. I. da S.; KATSURAYAMA, Y.; NICKEL, O.; FAJARDO, T. V. M. Manejo de doenças. In: FIORAVANÇO, J. C.; SANTOS, R. S. S. dos (Ed.). Maçã: o produtor pergunta, a Embrapa responde. Brasília, DF: Embrapa, 2013. p. 219-237 (Coleção 500 perguntas, 500 respostas).| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 217. | | MOURA, C. J. M. de; FAJARDO, T. V. M.; EIRAS, M.; SILVA, F. N. da; NICKEL, O. Molecular characterization of GSyV-1 and GLRaV-3 and prevalence of grapevine viruses in a grape-growing area. Scientia Agricola, Brasília,DF, v. 75, n. 1, p.43-51, jan./feb. 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 218. | | BASSO, M. F.; SILVA, J. C. F.; FAJARDO, T. V. M.; FONTES, E. P. B.; ZERBINI, F. M. A novel, highly divergent ssDNA virus identified in Brazil infecting apple, pear and grapevine. Virus Research, v. 14, n. 210, p. 27-33, July 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 219. | | SOUZA, E. B.; NICKEL, O.; NAGATA, T.; SILVA, J. M. F.; FAJARDO, T. V. M.; BARROS, D. R. Recombination events in full genome sequences of apple stem grooving virus. Virus Reviews and Research, v. 20, n. 2, p. 146, 2016. (Annals of the XXVII Brazilian Congress of Virology & XI Mercosur Meeting of Virology). Annals of the XXVII Brazilian Congress of Virology & XI Mercosur Meeting of Virology, Pirienópolis, GO, 2016.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 220. | | FELDBERG, N. P.; GROHS, D. S.; FAJARDO, T. V. M.; RITSCHEL, P. S.; MAIA, J. D. G. Produção e comercialização de gemas e estacas de videira para viveiristas: experiência da Embrapa nos últimos 10 anos. In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 27.; ENCONTRO NACIONAL SOBRE FRUTICULTURA DE CLIMA TEMPERADO, 17., 2022, Florianópolis, SC. Anais... + Ciência + Saúde. Florianópolis, SC: Epagri, 25 a 29 abril de 2022. p. 1204-1208.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| Registros recuperados : 283 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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