| |
|
|
| Registros recuperados : 587 | |
| Registros recuperados : 587 | |
|
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Pecuária Sudeste. |
|
Data corrente: |
24/02/2017 |
|
Data da última atualização: |
30/08/2018 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
A - 1 |
|
Autoria: |
GIGLIOTI, R.; OLIVEIRA, H. N.; IBELLI, A. M. G.; BILHASSI, T. B.; NEO, T. A.; SANTANA, C. H.; RABELO, M. D.; MACHADO, R. Z.; CHAGAS, A. C. de S.; OLIVEIRA, M. C. de S. |
|
Afiliação: |
Rodrigo Giglioti, UNESP; Henrique Nunes Oliveira, UNESP; ADRIANA MERCIA GUARATINI IBELLI, CNPSA; Talita Barban Bilhassi, UNESP; Thalita Athiê Néo, UFSCar; Clarissa Helena Santana, UNESP; MARCIO DIAS RABELO, CPPSE; Rosângela Zacarias Machado, UNESP; ANA CAROLINA DE SOUZA CHAGAS, CPPSE; MARCIA CRISTINA DE SENA OLIVEIRA, CPPSE. |
|
Título: |
Neither quantification by qPCR nor quantitative Elisa can be used to discriminate angus cattle for resistance/susceptibility to Babesia bovis. |
|
Ano de publicação: |
2017 |
|
Fonte/Imprenta: |
Ticks and Tick-borne Diseases, v. 8, n. 3, p. 335-340, 2017. |
|
DOI: |
http://dx.doi.org/10.1016/j.ttbdis.2016.11.008 |
|
Idioma: |
Inglês |
|
Conteúdo: |
With the aim of finding quantitative phenotypic traits that can be used to discriminate the levels of resistance/susceptibility to Babesia bovis, we estimated the repeatability and correlation between the level of infection, determined by the number of copies of a fragment of the gene that encodes cytochrome B (NC mt-cyB) of B. bovis, and the levels of the anti-B. bovis antibodies, in blood samples collected from 51 Angus cattle on two different occasions. Samples with the anticoagulant EDTA were used for DNA extraction and without anticoagulant for separation of the blood serum. The quantification of the NC mt-cyB of B. bovis was carried out by the quantitative PCR technique (qPCR), while the anti-B. bovis IgG antibody titers (S/P) were quantified by the ELISA method. The NC and S/P data were log10-transformed to improve the approximation to the normal distribution and were analyzed using mixed models. The correlations between NC mt-cyB and S/P were estimated, as well as the repeatability values for each trait. The results obtainedshowedthehighsensitivity ofthe techniques, with100%ofthe animals beingpositive for B. bovis, detected by both the serological and molecular tests. The correlations estimated between NC and S/P were low, 0.10 and 0.12, in the first and second collection, respectively. The repeatability estimated for NC was 0.06, whereas for the S/P it was 0.42. The low correlations between S/P and NC in the two collections demonstrated that the variation in the NC value is independent of the level of antibodies. This results indicated that animals with a higher levels of antibodies against B. bovis in the first collection continued to have a higher levels in the second one. However, the very low values for the repeatability value of NC, and for the correlations between S/P and NC, demonstrates that neither NC or S/P could be used to discriminate animals for resistance/susceptibility to B. bovis. MenosWith the aim of finding quantitative phenotypic traits that can be used to discriminate the levels of resistance/susceptibility to Babesia bovis, we estimated the repeatability and correlation between the level of infection, determined by the number of copies of a fragment of the gene that encodes cytochrome B (NC mt-cyB) of B. bovis, and the levels of the anti-B. bovis antibodies, in blood samples collected from 51 Angus cattle on two different occasions. Samples with the anticoagulant EDTA were used for DNA extraction and without anticoagulant for separation of the blood serum. The quantification of the NC mt-cyB of B. bovis was carried out by the quantitative PCR technique (qPCR), while the anti-B. bovis IgG antibody titers (S/P) were quantified by the ELISA method. The NC and S/P data were log10-transformed to improve the approximation to the normal distribution and were analyzed using mixed models. The correlations between NC mt-cyB and S/P were estimated, as well as the repeatability values for each trait. The results obtainedshowedthehighsensitivity ofthe techniques, with100%ofthe animals beingpositive for B. bovis, detected by both the serological and molecular tests. The correlations estimated between NC and S/P were low, 0.10 and 0.12, in the first and second collection, respectively. The repeatability estimated for NC was 0.06, whereas for the S/P it was 0.42. The low correlations between S/P and NC in the two collections demonstrated that the variation in the NC ... Mostrar Tudo |
|
Palavras-Chave: |
Resistance. |
|
Thesagro: |
Babesia Bovis. |
|
Thesaurus NAL: |
beef cattle. |
|
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
|
Marc: |
LEADER 02829naa a2200277 a 4500
001 2065568
005 2018-08-30
008 2017 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.1016/j.ttbdis.2016.11.008$2DOI
100 1 $aGIGLIOTI, R.
245 $aNeither quantification by qPCR nor quantitative Elisa can be used to discriminate angus cattle for resistance/susceptibility to Babesia bovis.$h[electronic resource]
260 $c2017
520 $aWith the aim of finding quantitative phenotypic traits that can be used to discriminate the levels of resistance/susceptibility to Babesia bovis, we estimated the repeatability and correlation between the level of infection, determined by the number of copies of a fragment of the gene that encodes cytochrome B (NC mt-cyB) of B. bovis, and the levels of the anti-B. bovis antibodies, in blood samples collected from 51 Angus cattle on two different occasions. Samples with the anticoagulant EDTA were used for DNA extraction and without anticoagulant for separation of the blood serum. The quantification of the NC mt-cyB of B. bovis was carried out by the quantitative PCR technique (qPCR), while the anti-B. bovis IgG antibody titers (S/P) were quantified by the ELISA method. The NC and S/P data were log10-transformed to improve the approximation to the normal distribution and were analyzed using mixed models. The correlations between NC mt-cyB and S/P were estimated, as well as the repeatability values for each trait. The results obtainedshowedthehighsensitivity ofthe techniques, with100%ofthe animals beingpositive for B. bovis, detected by both the serological and molecular tests. The correlations estimated between NC and S/P were low, 0.10 and 0.12, in the first and second collection, respectively. The repeatability estimated for NC was 0.06, whereas for the S/P it was 0.42. The low correlations between S/P and NC in the two collections demonstrated that the variation in the NC value is independent of the level of antibodies. This results indicated that animals with a higher levels of antibodies against B. bovis in the first collection continued to have a higher levels in the second one. However, the very low values for the repeatability value of NC, and for the correlations between S/P and NC, demonstrates that neither NC or S/P could be used to discriminate animals for resistance/susceptibility to B. bovis.
650 $abeef cattle
650 $aBabesia Bovis
653 $aResistance
700 1 $aOLIVEIRA, H. N.
700 1 $aIBELLI, A. M. G.
700 1 $aBILHASSI, T. B.
700 1 $aNEO, T. A.
700 1 $aSANTANA, C. H.
700 1 $aRABELO, M. D.
700 1 $aMACHADO, R. Z.
700 1 $aCHAGAS, A. C. de S.
700 1 $aOLIVEIRA, M. C. de S.
773 $tTicks and Tick-borne Diseases$gv. 8, n. 3, p. 335-340, 2017.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Expressão de busca inválida. Verifique!!! |
|
|
