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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
17/09/2012 |
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Data da última atualização: |
25/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FEDERICI, M. T.; PICCHI, S. C.; STUCHI, E. S.; FADEL, A. L.; LAIA, M. L.; LEMOS, M. V. F.; LEMOS, E. G. M. |
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Afiliação: |
MARÍA TERESA FEDERICI, INIA; SIMONE CRISTINA PICCHI, UNESP; EDUARDO SANCHES STUCHI, CNPMF; ANDRÉ LUIZ FADEL, ESALQ; MARCELO LUIZ LAIA, UFVJM; MANOEL VICTOR FRANCO LEMOS, UNESP; ELIANA GERTRUDES MACEDO LEMOS, UNESP. |
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Título: |
Xylella fastidiosa: An in vivo system to study possible survival strategies within citrus xylem vessels based on global gene expression analysis. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Electronic Journal of Biotechnology, 2012. |
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ISSN: |
0717-3458 |
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DOI: |
10.2225/vol15-issue3-fulltext-4 |
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Idioma: |
Inglês |
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Conteúdo: |
Xylella fastidiosa inhabits the plant xylem, a nutrient-poor environment, so that mechanisms to sense and respond to adverse environmental conditions are extremely important for bacterial survival in the plant host. Although the complete genome sequences of different Xylella strains have been determined, little is known about stress responses and gene regulation in these organisms. In this work, a DNA microarray was constructed containing 2,600 ORFs identified in the genome sequencing project of Xylella fastidiosa 9a5c strain, and used to check global gene expression differences in the bacteria when it is infecting a symptomatic and a tolerant citrus tree. Different patterns of expression were found in each variety, suggesting that bacteria are responding differentially according to each plant xylem environment. The global gene expression profile was determined and several genes related to bacterial survival in stressed conditions were found to be differentially expressed between varieties, suggesting the involvement of different strategies for adaptation to the environment. The expression pattern of some genes related to the heat shock response, toxin and detoxification processes, adaptation to atypical conditions, repair systems as well as some regulatory genes are discussed in this paper. DNA microarray proved to be a powerful technique for global transcriptome analyses. This is one of the first studies of Xylella fastidiosa gene expression in vivo which helped to increase insight into stress responses and possible bacterial survival mechanisms in the nutrient-poor environment of xylem vessels. MenosXylella fastidiosa inhabits the plant xylem, a nutrient-poor environment, so that mechanisms to sense and respond to adverse environmental conditions are extremely important for bacterial survival in the plant host. Although the complete genome sequences of different Xylella strains have been determined, little is known about stress responses and gene regulation in these organisms. In this work, a DNA microarray was constructed containing 2,600 ORFs identified in the genome sequencing project of Xylella fastidiosa 9a5c strain, and used to check global gene expression differences in the bacteria when it is infecting a symptomatic and a tolerant citrus tree. Different patterns of expression were found in each variety, suggesting that bacteria are responding differentially according to each plant xylem environment. The global gene expression profile was determined and several genes related to bacterial survival in stressed conditions were found to be differentially expressed between varieties, suggesting the involvement of different strategies for adaptation to the environment. The expression pattern of some genes related to the heat shock response, toxin and detoxification processes, adaptation to atypical conditions, repair systems as well as some regulatory genes are discussed in this paper. DNA microarray proved to be a powerful technique for global transcriptome analyses. This is one of the first studies of Xylella fastidiosa gene expression in vivo which helped to increas... Mostrar Tudo |
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Palavras-Chave: |
Microarray. |
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Thesagro: |
Xylella Fastidiosa. |
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Thesaurus Nal: |
Citrus. |
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Categoria do assunto: |
-- |
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Marc: |
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100 1 $aFEDERICI, M. T.
245 $aXylella fastidiosa$bAn in vivo system to study possible survival strategies within citrus xylem vessels based on global gene expression analysis.$h[electronic resource]
260 $c2012
520 $aXylella fastidiosa inhabits the plant xylem, a nutrient-poor environment, so that mechanisms to sense and respond to adverse environmental conditions are extremely important for bacterial survival in the plant host. Although the complete genome sequences of different Xylella strains have been determined, little is known about stress responses and gene regulation in these organisms. In this work, a DNA microarray was constructed containing 2,600 ORFs identified in the genome sequencing project of Xylella fastidiosa 9a5c strain, and used to check global gene expression differences in the bacteria when it is infecting a symptomatic and a tolerant citrus tree. Different patterns of expression were found in each variety, suggesting that bacteria are responding differentially according to each plant xylem environment. The global gene expression profile was determined and several genes related to bacterial survival in stressed conditions were found to be differentially expressed between varieties, suggesting the involvement of different strategies for adaptation to the environment. The expression pattern of some genes related to the heat shock response, toxin and detoxification processes, adaptation to atypical conditions, repair systems as well as some regulatory genes are discussed in this paper. DNA microarray proved to be a powerful technique for global transcriptome analyses. This is one of the first studies of Xylella fastidiosa gene expression in vivo which helped to increase insight into stress responses and possible bacterial survival mechanisms in the nutrient-poor environment of xylem vessels.
650 $aCitrus
650 $aXylella Fastidiosa
653 $aMicroarray
700 1 $aPICCHI, S. C.
700 1 $aSTUCHI, E. S.
700 1 $aFADEL, A. L.
700 1 $aLAIA, M. L.
700 1 $aLEMOS, M. V. F.
700 1 $aLEMOS, E. G. M.
773 $tElectronic Journal of Biotechnology, 2012.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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| Registros recuperados : 16 | |
| 5. |  | FADEL, A. L.; DELLA COLETTA FILHO, H.; RODRIGUES, C. M.; RODRIGUES, M. T. F.; STUCHI, E. S. Reação da cultivar Navelina ISA 315 (Citrus sinensis L. Osb.), à clorose variegada dos citros em condições de campo. In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 21., 2010, Natal. Frutas: saúde, inovação e responsabilidade: anais. Natal: Sociedade Brasileira de Fruticultura, 2010. 1 CD-ROM. pdf 128| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 6. | | ASTUA, J. de F.; FADEL, A. L.; BASTIANEL, M.; NOVELLI, V. M.; ANTONIOLI-LUIZON, R.; MACHADO, M. A. Resposta diferencial de espécies e de híbridos de citros à leprose. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 43, n. 7, p. 809-814, jul. 2008. Título em inglês: Differential response of citrus species and hybrids to leprosis.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 7. | | FREITAS-ÁSTUA, J.; FADEL, A. L.; BASTIANEL, M.; NOVELLI, V. M.; ANTONIOLI-LUIZON, R.; MACHADO, M. A. Resposta diferencial de espécies e de híbridos de citros à leprose. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 43, n. 7, p. 809-814, jul. 2008.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 10. | | RAMOS, Y. C.; MOURÃO FILHO, F. de A. A.; STUCHI, E. S.; SENTELHAS, P. C.; FADEL, A. L. Sensibilidade de laranjeiras-doces ao deficit hídrico. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 51, n. 1, p. 86-89, jan. 2016 Notas científicas. Título em inglês: Sweet orange selections? sensitivity to water deficit.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura; Embrapa Unidades Centrais. |
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| 11. | | RAMOS, Y. C.; MOURÃO FILHO, F. A. A.; STUCHI, E. S.; SILVA, S. R; CAPUTO, M. M.; BREMER NETO, H.; FADEL, A. L. Initial production of 17 mid-season sweet orange cultivars in Southwestern São Paulo State, Brazil. In: INTERNATIONAL CITRUS CONGRESS, 22., 2012.Valencia. Book of abstracts. Valencia: International Society of Citriculture; Instituto Valenciano de Investigaciones Agrarias; Fundación Agroalimed, 2012. Documento eletrônico.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 12. | | COSTA, D. P; STUCHI, E. S.; GIRARDI, E. A.; RAMOS, Y. C.; FADEL, A. L.; PASSOS, O. S.; GESTEIRA, A. da S.; SOARES FILHO, W. dos S. Potential rootstocks for valencia sweet orange under rain-fed cultivation in northern São Paulo State, Brazil. In: INTERNATIONAL CITRUS CONGRESS, 2016, Foz do Iguaçu. Abstract book. Campinas: IAC; Londrina: IAPAR, 2016. p.175| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 14. | | COSTA, D. P.; STUCHI, E. S.; GIRARDI, E. A.; RAMOS, Y. C.; FADEL, A. L.; MALDONADO JUNIOR, W.; GESTEIRA, A. da S.; PASSOS, O. S.; SOARES FILHO, W. dos S. Potential rootstocks for Valencia sweet orange in rain-fed cultivation in the North of São Paulo, Brazil. Citrus Research & Technology, v. 37, n. 1, 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 15. | | SOARES FILHO, W. dos S.; RAMOS, Y. C.; SILVA, A. L. V.; FADEL, A. L.; STUCHI, E. S.; GIRARDI, E. A.; LEÃO, H. C.; GESTEIRA, A. da S.; PASSOS, O. S. Dwarfing rootstocks for Valencia sweet orange. In: INTERNATIONAL HORTICULTURAL CONGRESS, 29., 2014, Brisbane. Sustaining lives, livelihoods and landscapes. [Abstracts...]. Brisbane: AuSHS : ISHS, 2014.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 16. | | COSTA, D. P.; STUCHI, E. S.; GIRARDI, E. A.; GESTEIRA, A. da S.; COELHO FILHO, M. A.; LEDO, C. A. da S.; FADEL, A. L.; SILVA, A. L. V. da; LEÃO, H. C. de; RAMOS, Y. C.; PASSOS, O. S.; SOARES FILHO, W. dos S. Hybrid Rootstocks for Valencia Sweet Orange in Rainfed Cultivation Under Tropical Savannah Climate. Agricultural Science, v. 12, n.. 11, 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| Registros recuperados : 16 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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