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Registro Completo |
Biblioteca(s): |
Embrapa Agrobiologia. |
Data corrente: |
12/06/2023 |
Data da última atualização: |
11/07/2023 |
Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
Autoria: |
GUERRA, J. G. M.; ESPINDOLA, J. A. A.; ARAUJO, E. da S.; LEAL, M. A. de A.; ABBOUD, A. C. de S.; ALMEIDA, D. L. de; POLLI, H. de; NEVES, M. C. P.; RIBEIRO, R. de L. D. |
Afiliação: |
JOSE GUILHERME MARINHO GUERRA, CNPAB; JOSE ANTONIO AZEVEDO ESPINDOLA, CNPAB; EDNALDO DA SILVA ARAUJO, CNPAB; MARCO ANTONIO DE ALMEIDA LEAL, CNPAB; ANTÔNIO CARLOS DE SOUZA ABBOUD, UFRRJ; DEJAIR LOPES DE ALMEIDA, CNPAB; HELVECIO DE POLLI, CNPAB; MARIA CRISTINA PRATA NEVES, CNPAB; RAUL DE LUCENA DUARTE RIBEIRO, UFRRJ. |
Título: |
Adubação verde no cultivo de hortaliças. |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
In: LIMA FILHO, O. F. de; AMBROSANO, E. J. WUTKE, E. B.; ROSSI, F.; CARLOS, J. A. D. (ed.). Adubação verde e plantas de cobertura no Brasil: fundamentos e prática. 2. ed. rev. e atual. Brasília, DF: Embrapa, 2023. |
Volume: |
v. 2 |
Páginas: |
p. 265-291. |
ISBN: |
978-65-86056-62-4 v. 2 |
Idioma: |
Português |
Conteúdo: |
Os resultados discutidos neste capítulo referem-se a trabalhos de pesquisa de caráter tecnológico, que buscam oferecer opções técnicas para subsidiar agentes de desenvolvimento e agricultores na tomada de decisão sobre quando e como fazer uso da adubação verde. Para tanto, são abordadas distintas modalidades e estratégias de manejo de plantas de cobertura do solo, enfatizando-se arranjos populacionais, rotações e consórcios, faixas intercalares, coberturas mortas e compostagem. |
Thesagro: |
Adubo Verde; Batata Doce; Crotalária Juncea; Feijão de Porco; Guandu; Hortaliça; Leguminosa; Mucuna Preta; Produtividade. |
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1154391/1/Adubacao-verde-no-cultivo-de-hortalica.pdf
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Marc: |
LEADER 01591naa a2200349 a 4500 001 2154391 005 2023-07-11 008 2023 bl uuuu u00u1 u #d 100 1 $aGUERRA, J. G. M. 245 $aAdubação verde no cultivo de hortaliças.$h[electronic resource] 260 $c2023 300 $ap. 265-291. v. 2 490 $vv. 2 520 $aOs resultados discutidos neste capítulo referem-se a trabalhos de pesquisa de caráter tecnológico, que buscam oferecer opções técnicas para subsidiar agentes de desenvolvimento e agricultores na tomada de decisão sobre quando e como fazer uso da adubação verde. Para tanto, são abordadas distintas modalidades e estratégias de manejo de plantas de cobertura do solo, enfatizando-se arranjos populacionais, rotações e consórcios, faixas intercalares, coberturas mortas e compostagem. 650 $aAdubo Verde 650 $aBatata Doce 650 $aCrotalária Juncea 650 $aFeijão de Porco 650 $aGuandu 650 $aHortaliça 650 $aLeguminosa 650 $aMucuna Preta 650 $aProdutividade 700 1 $aESPINDOLA, J. A. A. 700 1 $aARAUJO, E. da S. 700 1 $aLEAL, M. A. de A. 700 1 $aABBOUD, A. C. de S. 700 1 $aALMEIDA, D. L. de 700 1 $aPOLLI, H. de 700 1 $aNEVES, M. C. P. 700 1 $aRIBEIRO, R. de L. D. 773 $tIn: LIMA FILHO, O. F. de; AMBROSANO, E. J. WUTKE, E. B.; ROSSI, F.; CARLOS, J. A. D. (ed.). Adubação verde e plantas de cobertura no Brasil: fundamentos e prática. 2. ed. rev. e atual. Brasília, DF: Embrapa, 2023.
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Embrapa Agrobiologia (CNPAB) |
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Registro Completo
Biblioteca(s): |
Embrapa Suínos e Aves. |
Data corrente: |
24/09/2018 |
Data da última atualização: |
24/09/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
OLIVEIRA FILHO, J. X. de; MORES, M. A. Z.; REBELATTO, R.; KICH, J. D.; CANTAO, M. E.; KLEIN, C. S.; GUEDES, R. M. C.; COLDEBELLA, A.; BARCELLOS, D. E. S. N. de; MORES, N. |
Afiliação: |
JOÃO XAVIER DE OLIVEIRA FILHO; MARCOS ANTONIO ZANELLA MORES, CNPSA; RAQUEL REBELATTO, CNPSA; JALUSA DEON KICH, CNPSA; MAURICIO EGIDIO CANTAO, CNPSA; CATIA SILENE KLEIN, CNPSA; ROBERTO MAURÍCIO CARVALHO GUEDES; ARLEI COLDEBELLA, CNPSA; DAVID EMÍLIO SANTOS NEVES de BARCELLOS; NELSON MORES, CNPSA. |
Título: |
Pathogenic variability among Pasteurella multocida type A isolates from Brazilian pig farms. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
BMC Veterinary Research, v. 14, n. 244, 2018. |
DOI: |
10.1186/s12917-018-1565-2 |
Idioma: |
Inglês |
Conteúdo: |
Abstract: Background: Pasteurella multocida type A (PmA) is considered a secondary agent of pneumonia in pigs. The role of PmA as a primary pathogen was investigated by challenging pigs with eight field strains isolated from pneumonia and serositis in six Brazilian states. Eight groups of eight pigs each were intranasally inoculated with different strains of PmA (1.5 mL/nostril of 10e7 CFU/mL). The control group (n = 12) received sterile PBS. The pigs were euthanized by electrocution and necropsied by 5 dpi. Macroscopic lesions were recorded, and swabs and fragments of thoracic and abdominal organs were analyzed by bacteriological and pathological assays. The PmA strains were analyzed for four virulence genes (toxA: toxin; pfhA: adhesion; tbpA and hgbB: iron acquisition) by PCR and sequencing and submitted to multilocus sequence typing (MLST). Results: The eight PmA strains were classified as follows: five as highly pathogenic (HP) for causing necrotic bronchopneumonia and diffuse fibrinous pleuritis and pericarditis; one as low pathogenic for causing only focal bronchopneumonia; and two as nonpathogenic because they did not cause injury to any pig. PCR for the gene pfhA was positive for all five HP isolates. Sequencing demonstrated that the pfhA region of the HP strains comprised four genes: tpsB1, pfhA1, tpsB2 and pfhA2. The low and nonpathogenic strains did not contain the genes tpsB2 and pfhA2. A deletion of four bases was observed in the pfhA gene in the low pathogenic strain, and an insertion of 37 kb of phage DNA was observed in the nonpathogenic strains. MLST clustered the HP isolates in one group and the low and nonpathogenic isolates in another. Only the nonpathogenic isolates matched sequence type 10; the other isolates did not match any type available in the MLST database. Conclusions: The hypothesis that some PmA strains are primary pathogens and cause disease in pigs without any co-factor was confirmed. The pfhA region, comprising the genes tpsB1, tpsB2, pfhA1 and pfhA2, is related to the pathogenicity of PmA. The HP strains can cause necrotic bronchopneumonia, fibrinous pleuritis and pericarditis in pigs and can be identified by PCR amplification of the gene pfhA2. MenosAbstract: Background: Pasteurella multocida type A (PmA) is considered a secondary agent of pneumonia in pigs. The role of PmA as a primary pathogen was investigated by challenging pigs with eight field strains isolated from pneumonia and serositis in six Brazilian states. Eight groups of eight pigs each were intranasally inoculated with different strains of PmA (1.5 mL/nostril of 10e7 CFU/mL). The control group (n = 12) received sterile PBS. The pigs were euthanized by electrocution and necropsied by 5 dpi. Macroscopic lesions were recorded, and swabs and fragments of thoracic and abdominal organs were analyzed by bacteriological and pathological assays. The PmA strains were analyzed for four virulence genes (toxA: toxin; pfhA: adhesion; tbpA and hgbB: iron acquisition) by PCR and sequencing and submitted to multilocus sequence typing (MLST). Results: The eight PmA strains were classified as follows: five as highly pathogenic (HP) for causing necrotic bronchopneumonia and diffuse fibrinous pleuritis and pericarditis; one as low pathogenic for causing only focal bronchopneumonia; and two as nonpathogenic because they did not cause injury to any pig. PCR for the gene pfhA was positive for all five HP isolates. Sequencing demonstrated that the pfhA region of the HP strains comprised four genes: tpsB1, pfhA1, tpsB2 and pfhA2. The low and nonpathogenic strains did not contain the genes tpsB2 and pfhA2. A deletion of four bases was observed in the pfhA gene in the low pathogenic ... Mostrar Tudo |
Palavras-Chave: |
Broncopneumonia; PCR; PfhA; Poliserositis; Polyserositis. |
Thesagro: |
Doença Respiratória; Suíno. |
Thesaurus NAL: |
Bronchopneumonia; Multilocus sequence typing; Pathogenicity; Polymerase chain reaction; Respiratory tract diseases; Swine; Virulent strains. |
Categoria do assunto: |
-- |
Marc: |
LEADER 03381naa a2200409 a 4500 001 2096196 005 2018-09-24 008 2018 bl uuuu u00u1 u #d 024 7 $a10.1186/s12917-018-1565-2$2DOI 100 1 $aOLIVEIRA FILHO, J. X. de 245 $aPathogenic variability among Pasteurella multocida type A isolates from Brazilian pig farms.$h[electronic resource] 260 $c2018 520 $aAbstract: Background: Pasteurella multocida type A (PmA) is considered a secondary agent of pneumonia in pigs. The role of PmA as a primary pathogen was investigated by challenging pigs with eight field strains isolated from pneumonia and serositis in six Brazilian states. Eight groups of eight pigs each were intranasally inoculated with different strains of PmA (1.5 mL/nostril of 10e7 CFU/mL). The control group (n = 12) received sterile PBS. The pigs were euthanized by electrocution and necropsied by 5 dpi. Macroscopic lesions were recorded, and swabs and fragments of thoracic and abdominal organs were analyzed by bacteriological and pathological assays. The PmA strains were analyzed for four virulence genes (toxA: toxin; pfhA: adhesion; tbpA and hgbB: iron acquisition) by PCR and sequencing and submitted to multilocus sequence typing (MLST). Results: The eight PmA strains were classified as follows: five as highly pathogenic (HP) for causing necrotic bronchopneumonia and diffuse fibrinous pleuritis and pericarditis; one as low pathogenic for causing only focal bronchopneumonia; and two as nonpathogenic because they did not cause injury to any pig. PCR for the gene pfhA was positive for all five HP isolates. Sequencing demonstrated that the pfhA region of the HP strains comprised four genes: tpsB1, pfhA1, tpsB2 and pfhA2. The low and nonpathogenic strains did not contain the genes tpsB2 and pfhA2. A deletion of four bases was observed in the pfhA gene in the low pathogenic strain, and an insertion of 37 kb of phage DNA was observed in the nonpathogenic strains. MLST clustered the HP isolates in one group and the low and nonpathogenic isolates in another. Only the nonpathogenic isolates matched sequence type 10; the other isolates did not match any type available in the MLST database. Conclusions: The hypothesis that some PmA strains are primary pathogens and cause disease in pigs without any co-factor was confirmed. The pfhA region, comprising the genes tpsB1, tpsB2, pfhA1 and pfhA2, is related to the pathogenicity of PmA. The HP strains can cause necrotic bronchopneumonia, fibrinous pleuritis and pericarditis in pigs and can be identified by PCR amplification of the gene pfhA2. 650 $aBronchopneumonia 650 $aMultilocus sequence typing 650 $aPathogenicity 650 $aPolymerase chain reaction 650 $aRespiratory tract diseases 650 $aSwine 650 $aVirulent strains 650 $aDoença Respiratória 650 $aSuíno 653 $aBroncopneumonia 653 $aPCR 653 $aPfhA 653 $aPoliserositis 653 $aPolyserositis 700 1 $aMORES, M. A. Z. 700 1 $aREBELATTO, R. 700 1 $aKICH, J. D. 700 1 $aCANTAO, M. E. 700 1 $aKLEIN, C. S. 700 1 $aGUEDES, R. M. C. 700 1 $aCOLDEBELLA, A. 700 1 $aBARCELLOS, D. E. S. N. de 700 1 $aMORES, N. 773 $tBMC Veterinary Research$gv. 14, n. 244, 2018.
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