Data/hora: 24/05/2024 15:52:50

Biblioteca(s): 	Embrapa Mandioca e Fruticultura.	
Data corrente: 	20/04/1994	
Data da última atualização: 	20/04/1994	
Autoria: 	ESCOBAR, R. H.; ROCA, W, M.; MAFLA, G.	
Afiliação: 	CIAT, Cali, Colombia.	
Título: 	Cryopreservation of cassava shoot tips.	
Ano de publicação: 	1993	
Fonte/Imprenta: 	Cali, Colombia: CIAT, 1993	
Páginas: 	116-121p	
Idioma: 	Inglês	
Notas: 	International scientific meeting Cassava Biotechnology Network, 1, 1992, Cartajena, Colombia. Proceedinjs... Cali, Colombia: CIAT, 1993.	
Conteúdo: 	Currently, ex-situ conservation of cassava germplasm at CIAT is carried out both in the field and laboratory as shoot tipo cultures. The in vitro conservation constitutes an active collection and clones are sub-cultured and re-cycled every 12-18 months depending on the genotype. The availability of long-term storage of cassava germplasm in reduced space, free of genetic change, and at a low cost can be achieved by cryopreservation. A research proiect on cassava cryopreservation began at CIAT in 1988; the project comprises three phases: (i) the first phase was carried out in cooperation with IBPGR (1988-90) and resulted in the recovery of plants from frozen shoot tips in liquid nitrogen. With the cv. MCol 22, recovery rates ranged from 20 to 40%. however, se   veral cassava cvs. tested only showed a low response or did not respond at all (Ann. Report, CIAT 1991). (ii) the second phase of the research (1990-present), was designed to improved the previous protocol in order to increase the recovery rate of plants, minimizing genotypic differences . Lower temperature and higher illumination of donor cultures increased recovery from liquid nitrogen with consisten rates 50- 60%. Use of high concentration of sucrose in lieu of sorbitol and DMSO in the pre-culture stage, allowed high rated of plant recovery from frozen shoot tips. Work continues to adjust the cryoprotection, freezing, and post-thawing culture phases. 	
Palavras-Chave: 	Alimentacao humana; Congresso.	
Thesagro: 	Biotecnologia; Germoplasma; Melhoramento.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Mandioca e Fruticultura (CNPMF)	
Exemplares: 	CNPMF - (13425-1) - ADD - PL - -- - 633.6820601 - I61p - 1993000070 - DIS