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Registro Completo |
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Biblioteca(s): |
Embrapa Amapá; Embrapa Amazônia Oriental; Embrapa Mandioca e Fruticultura; Embrapa Meio-Norte; Embrapa Unidades Centrais. |
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Data corrente: |
18/08/1997 |
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Data da última atualização: |
18/05/2023 |
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Autoria: |
EMBRAPA. Centro Nacional de Pesquisa de Mandioca e Fruticultura. |
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Título: |
EMBRAPA - pesquisa para o desenvolvimento Cruz das Almas - BA. |
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Ano de publicação: |
1987 |
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Fonte/Imprenta: |
Cruz das Almas: Embrapa Mandioca e Fruticultura, 1987. |
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Série: |
(Embrapa Mandioca e Fruticultura. Documentos, 20). |
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ISSN: |
0101-7411 |
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Idioma: |
Português |
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Notas: |
Memória. |
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Conteúdo: |
EMBRAPA: Instituições/ Finalidades/ Atividades. EMBRAPA: Unidades Centrais. EMBRAPA: Unidades Descentralizadas. CNPMF: Objetivos/ Implantação. CNPMF: Localização. Programas Nacionais de Pesquisa. SID. CNPMF: Administração. CNPMF: Assistencia Social. CRUZ DAS ALMAS. Primórdios/Aspectos Históricos. Aspectos Físicos/ Geográficos. Aspectos Culturais. Aspectos Religiosos. Imprensa/Literatura/Artes. Saúde/Assistencai Médica. Comércio/Indústria. Aspectos Sociais/ Esportivos. Governo. Hospedagem. Monumentos. Hino a Cruz das Almas. |
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Palavras-Chave: |
Atividades; Bahia; Brasil; Cruz das Almas; Embrapa; Embrapa Semi-Árido; Fruit; Instituto de pesquisa; Pesquisa agropecuaria; Social conditions; Valor cultural. |
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Thesagro: |
Condição Social; Divulgação; Fruta; Historia; Pesquisa. |
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Thesaurus Nal: |
cultural values; history; research. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/80609/1/Embrapa-Pesquisa-Documentos-20-1987.pdf
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Marc: |
LEADER 01574nam a2200373 a 4500
001 1955058
005 2023-05-18
008 1987 bl uuuu 00u1 u #d
022 $a0101-7411
100 1 $aEMBRAPA. Centro Nacional de Pesquisa de Mandioca e Fruticultura.
245 $aEMBRAPA - pesquisa para o desenvolvimento Cruz das Almas - BA.
260 $aCruz das Almas: Embrapa Mandioca e Fruticultura$c1987
490 $a(Embrapa Mandioca e Fruticultura. Documentos, 20).
500 $aMemória.
520 $aEMBRAPA: Instituições/ Finalidades/ Atividades. EMBRAPA: Unidades Centrais. EMBRAPA: Unidades Descentralizadas. CNPMF: Objetivos/ Implantação. CNPMF: Localização. Programas Nacionais de Pesquisa. SID. CNPMF: Administração. CNPMF: Assistencia Social. CRUZ DAS ALMAS. Primórdios/Aspectos Históricos. Aspectos Físicos/ Geográficos. Aspectos Culturais. Aspectos Religiosos. Imprensa/Literatura/Artes. Saúde/Assistencai Médica. Comércio/Indústria. Aspectos Sociais/ Esportivos. Governo. Hospedagem. Monumentos. Hino a Cruz das Almas.
650 $acultural values
650 $ahistory
650 $aresearch
650 $aCondição Social
650 $aDivulgação
650 $aFruta
650 $aHistoria
650 $aPesquisa
653 $aAtividades
653 $aBahia
653 $aBrasil
653 $aCruz das Almas
653 $aEmbrapa
653 $aEmbrapa Semi-Árido
653 $aFruit
653 $aInstituto de pesquisa
653 $aPesquisa agropecuaria
653 $aSocial conditions
653 $aValor cultural
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
05/09/1994 |
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Data da última atualização: |
05/10/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ARAUJO, R. S.; ROBLETO, E. A.; HANDELSMAN, J. |
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Afiliação: |
RICARDO SILVA ARAUJO, CNPAF; EDUARDO A. ROBLETO; JO HANDELSMAN. |
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Título: |
A hydrophobic mutant of Rhizobium etli altered in nodulation competitiveness and growth in the rhizosphere. |
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Ano de publicação: |
1994 |
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Fonte/Imprenta: |
Applied and Environmental Microbiology, v. 60, n. 5, p. 1430-1436, May 1994. |
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DOI: |
https://doi.org/10.1128/aem.60.5.1430-1436.1994 |
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Idioma: |
Inglês |
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Conteúdo: |
We isolated and characterized CE3003, a Tn5-induced mutant with altered colony morphology derived from Rhizobium etli CE3. CE3003 produced domed colonies and was highly hydrophobic as indicated by its ability to partition into hexadecane, whereas its parent produced flat colonies and was hydrophilic. On bean plants, CE3003 induced nodules and reduced acetylene. CE3003 and CE3 grew at similar rates when they were grown separately or together in culture medium or inoculated singly onto bean seeds. However, when they were mixed at a 1:1 ratio and applied to seeds, CE3003 achieved significantly lower populations than CE3 in the rhizosphere. Five days after coinoculation of CE3 and CE3003, the population of the mutant was less than 10% of the population of CE3 in the bean rhizosphere. To determine the nodulation competitiveness of the mutant, it was coinoculated with CE3 at various ratios at planting, and the ratio of the nodules occupied by each strain was determined 21 days later. A 17,000-fold excess of CE3003 in mixed inocula was required to obtain equal nodule occupancy by the two strains. A genomic library of strain CE3 was mobilized into CE3003, and we identified a cosmid, pRA3003, that restored the parental colony morphology and hydrophilicity to the mutant. Restoration of the parental colony morphology was accompanied by recovery of the ability to grow competitively in the rhizosphere and to compete for nodulation of beans. The data show an association between cell surface hydrophobicity, nodulation competitiveness, and competitive growth in the rhizosphere in mutant CE3003. MenosWe isolated and characterized CE3003, a Tn5-induced mutant with altered colony morphology derived from Rhizobium etli CE3. CE3003 produced domed colonies and was highly hydrophobic as indicated by its ability to partition into hexadecane, whereas its parent produced flat colonies and was hydrophilic. On bean plants, CE3003 induced nodules and reduced acetylene. CE3003 and CE3 grew at similar rates when they were grown separately or together in culture medium or inoculated singly onto bean seeds. However, when they were mixed at a 1:1 ratio and applied to seeds, CE3003 achieved significantly lower populations than CE3 in the rhizosphere. Five days after coinoculation of CE3 and CE3003, the population of the mutant was less than 10% of the population of CE3 in the bean rhizosphere. To determine the nodulation competitiveness of the mutant, it was coinoculated with CE3 at various ratios at planting, and the ratio of the nodules occupied by each strain was determined 21 days later. A 17,000-fold excess of CE3003 in mixed inocula was required to obtain equal nodule occupancy by the two strains. A genomic library of strain CE3 was mobilized into CE3003, and we identified a cosmid, pRA3003, that restored the parental colony morphology and hydrophilicity to the mutant. Restoration of the parental colony morphology was accompanied by recovery of the ability to grow competitively in the rhizosphere and to compete for nodulation of beans. The data show an association between cell surfa... Mostrar Tudo |
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Palavras-Chave: |
Hydrophobic; Mutant; PTCA. |
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Thesagro: |
Bactéria; Mutante; Nodulação; Rhizobium. |
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Thesaurus NAL: |
nodulation; Rhizobium etli; rhizosphere. |
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Categoria do assunto: |
B Sociologia Rural |
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Marc: |
LEADER 02428naa a2200277 a 4500
001 1196158
005 2021-10-05
008 1994 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1128/aem.60.5.1430-1436.1994$2DOI
100 1 $aARAUJO, R. S.
245 $aA hydrophobic mutant of Rhizobium etli altered in nodulation competitiveness and growth in the rhizosphere.$h[electronic resource]
260 $c1994
520 $aWe isolated and characterized CE3003, a Tn5-induced mutant with altered colony morphology derived from Rhizobium etli CE3. CE3003 produced domed colonies and was highly hydrophobic as indicated by its ability to partition into hexadecane, whereas its parent produced flat colonies and was hydrophilic. On bean plants, CE3003 induced nodules and reduced acetylene. CE3003 and CE3 grew at similar rates when they were grown separately or together in culture medium or inoculated singly onto bean seeds. However, when they were mixed at a 1:1 ratio and applied to seeds, CE3003 achieved significantly lower populations than CE3 in the rhizosphere. Five days after coinoculation of CE3 and CE3003, the population of the mutant was less than 10% of the population of CE3 in the bean rhizosphere. To determine the nodulation competitiveness of the mutant, it was coinoculated with CE3 at various ratios at planting, and the ratio of the nodules occupied by each strain was determined 21 days later. A 17,000-fold excess of CE3003 in mixed inocula was required to obtain equal nodule occupancy by the two strains. A genomic library of strain CE3 was mobilized into CE3003, and we identified a cosmid, pRA3003, that restored the parental colony morphology and hydrophilicity to the mutant. Restoration of the parental colony morphology was accompanied by recovery of the ability to grow competitively in the rhizosphere and to compete for nodulation of beans. The data show an association between cell surface hydrophobicity, nodulation competitiveness, and competitive growth in the rhizosphere in mutant CE3003.
650 $anodulation
650 $aRhizobium etli
650 $arhizosphere
650 $aBactéria
650 $aMutante
650 $aNodulação
650 $aRhizobium
653 $aHydrophobic
653 $aMutant
653 $aPTCA
700 1 $aROBLETO, E. A.
700 1 $aHANDELSMAN, J.
773 $tApplied and Environmental Microbiology$gv. 60, n. 5, p. 1430-1436, May 1994.
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Embrapa Arroz e Feijão (CNPAF) |
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