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 | Acesso ao texto completo restrito à biblioteca da Embrapa Solos. Para informações adicionais entre em contato com cnps.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Solos. |
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Data corrente: |
20/09/2021 |
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Data da última atualização: |
20/09/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OLIVEIRA, V. M. de; MANFIO, G. P.; COUTINHO, H. L. da C.; KEIJZER-WOLTERS, A. C.; ELSAS, J. D. van. |
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Afiliação: |
VALÉRIA MAIA DE OLIVEIRA, UNICAMP; GILSON PAULO MANFIO, UNICAMP; HEITOR LUIZ DA COSTA COUTINHO, CNPS; ANNEKE CHRISTINA KEIJZER-WOLTERS, Plant Research International; JAN DIRK VAN ELSAS, University of Groningen. |
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Título: |
A ribosomal RNA gene intergenic spacer based PCR and DGGE fingerprinting method for the analysis of specific rhizobial communities in soil. |
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Ano de publicação: |
2006 |
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Fonte/Imprenta: |
Journal of Microbiological Methods, v. 64, n. 3, p. 366-379, Mar. 2006. |
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DOI: |
https://doi.org/10.1016/j.mimet.2005.05.015 |
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Idioma: |
Inglês |
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Conteúdo: |
A direct molecular method for assessing the diversity of specific populations of rhizobia in soil, based on nested PCR amplification of 16S-23S ribosomal RNA gene (rDNA) intergenic spacer (IGS) sequences, was developed. Initial generic amplification of bacterial rDNA IGS sequences from soil DNA was followed by specific amplification of (1) sequences affiliated with Rhizobium leguminosarum "sensu lato" and (2) R. tropici. Using analysis of the amplified sequences in clone libraries obtained on the basis of soil DNA, this two-sided method was shown to be very specific for rhizobial subpopulations in soil. It was then further validated as a direct fingerprinting tool of the target rhizobia based on denaturing gradient gel electrophoresis (DGGE). The PCR-DGGE approach was applied to soils from fields in Brazil cultivated with common bean (Phaseolus vulgaris) under conventional or no-tillage practices. The community fingerprints obtained allowed the direct analysis of the respective rhizobial community structures in soil samples from the two contrasting agricultural practices. Data obtained with both primer sets revealed clustering of the community structures of the target rhizobial types along treatment. Moreover, the DGGE profiles obtained with the R. tropici primer set indicated that the abundance and diversity of these organisms were favoured under NT practices. These results suggest that the R. leguminosarum-as well as R. tropici-targeted IGS-based nested PCR and DGGE are useful tools for monitoring the effect of agricultural practices on these and related rhizobial subpopulations in soils. MenosA direct molecular method for assessing the diversity of specific populations of rhizobia in soil, based on nested PCR amplification of 16S-23S ribosomal RNA gene (rDNA) intergenic spacer (IGS) sequences, was developed. Initial generic amplification of bacterial rDNA IGS sequences from soil DNA was followed by specific amplification of (1) sequences affiliated with Rhizobium leguminosarum "sensu lato" and (2) R. tropici. Using analysis of the amplified sequences in clone libraries obtained on the basis of soil DNA, this two-sided method was shown to be very specific for rhizobial subpopulations in soil. It was then further validated as a direct fingerprinting tool of the target rhizobia based on denaturing gradient gel electrophoresis (DGGE). The PCR-DGGE approach was applied to soils from fields in Brazil cultivated with common bean (Phaseolus vulgaris) under conventional or no-tillage practices. The community fingerprints obtained allowed the direct analysis of the respective rhizobial community structures in soil samples from the two contrasting agricultural practices. Data obtained with both primer sets revealed clustering of the community structures of the target rhizobial types along treatment. Moreover, the DGGE profiles obtained with the R. tropici primer set indicated that the abundance and diversity of these organisms were favoured under NT practices. These results suggest that the R. leguminosarum-as well as R. tropici-targeted IGS-based nested PCR and DGGE are us... Mostrar Tudo |
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Palavras-Chave: |
Culture-independent analysis; DGGE; Diversity; RDNA spacer; Rhizobia. |
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Thesaurus Nal: |
Soil management. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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Marc: |
LEADER 02461naa a2200253 a 4500
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024 7 $ahttps://doi.org/10.1016/j.mimet.2005.05.015$2DOI
100 1 $aOLIVEIRA, V. M. de
245 $aA ribosomal RNA gene intergenic spacer based PCR and DGGE fingerprinting method for the analysis of specific rhizobial communities in soil.$h[electronic resource]
260 $c2006
520 $aA direct molecular method for assessing the diversity of specific populations of rhizobia in soil, based on nested PCR amplification of 16S-23S ribosomal RNA gene (rDNA) intergenic spacer (IGS) sequences, was developed. Initial generic amplification of bacterial rDNA IGS sequences from soil DNA was followed by specific amplification of (1) sequences affiliated with Rhizobium leguminosarum "sensu lato" and (2) R. tropici. Using analysis of the amplified sequences in clone libraries obtained on the basis of soil DNA, this two-sided method was shown to be very specific for rhizobial subpopulations in soil. It was then further validated as a direct fingerprinting tool of the target rhizobia based on denaturing gradient gel electrophoresis (DGGE). The PCR-DGGE approach was applied to soils from fields in Brazil cultivated with common bean (Phaseolus vulgaris) under conventional or no-tillage practices. The community fingerprints obtained allowed the direct analysis of the respective rhizobial community structures in soil samples from the two contrasting agricultural practices. Data obtained with both primer sets revealed clustering of the community structures of the target rhizobial types along treatment. Moreover, the DGGE profiles obtained with the R. tropici primer set indicated that the abundance and diversity of these organisms were favoured under NT practices. These results suggest that the R. leguminosarum-as well as R. tropici-targeted IGS-based nested PCR and DGGE are useful tools for monitoring the effect of agricultural practices on these and related rhizobial subpopulations in soils.
650 $aSoil management
653 $aCulture-independent analysis
653 $aDGGE
653 $aDiversity
653 $aRDNA spacer
653 $aRhizobia
700 1 $aMANFIO, G. P.
700 1 $aCOUTINHO, H. L. da C.
700 1 $aKEIJZER-WOLTERS, A. C.
700 1 $aELSAS, J. D. van
773 $tJournal of Microbiological Methods$gv. 64, n. 3, p. 366-379, Mar. 2006.
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Registro original: |
Embrapa Solos (CNPS) |
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| Registros recuperados : 13 | |
| 1. |  | OLIVEIRA, V. M. de; COUTINHO, H. L. da C.; ELSAS, J. D. van; MANFIO, G. P. Avaliacao da diversidade de rizobios em amostras ambientais atraves do uso de primers especificos. In REUNIAO BRASILEIRA DE FERTILIDADE DE SOLO E NUTRICAO DE PLANTAS, 23.; REUNIAO BRASILEIRA SOBRE MICORRIZAS, 7.; SIMPOSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 5.; REUNIAO BRASILEIRA DE BIOLOGIA DO SOLO, 2., 1998, Caxambu,MG. Resumos... Lavras: UFLA / SBCS / SBM, 1998. p.808. FERTBIO 98: Interrelacao fertilidade, biologia de solo e nutricao de plantas: consolidacao de um paradigma.| Biblioteca(s): Embrapa Solos. |
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| 2. | | WEID, I. von der; PAIVA, E.; NOBREGA, A.; ELSAS, J. D. van; SELDIN, L. Diversity of Paenibacillus polymyxa strains isolated from the rhizosphere of maize planted in cerrado soil. Research in Microbiology, Paris, v. 151, n. 5, p. 369-381, 2000.| Tipo: Artigo em Periódico Indexado |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 3. | | COTTA, S. R.; DIAS, A. C. F.; MARRIEL, I. E.; GOMES, E. A.; ELSAS, J. D. van; SELDIN, L. Avaliação do possível impacto de milho geneticamente modificado na ciclagem do nitrogênio em solo de Cerrado. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 30.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 14.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 12.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 9.; SIMPÓSIO SOBRE SELÊNIO NO BRASIL, 1., 2012, Maceió. A responsabilidade socioambiental da pesquisa agrícola: anais. Viçosa, MG: Sociedade Brasileira de Ciência do Solo, 2012. 1 CD-ROM. Fertbio 2012.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 4. | | SELDIN, L.; ROSADO, A. S.; CRUZ, D. W. da; NOBREGA, A.; ELSAS, J. D. van; PAIVA, E. Comparison of Paenibacillus azotofixans strains isolated from rhizoplane, rhizosphere, and non-root-associated soil from maize planted in two different Brazilian soils. Applied and Environmental Microbiology, Washington, v. 64, n. 10, p. 3860-3868, 1998.| Tipo: Artigo em Periódico Indexado |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 5. | | COTTA, S. R.; DIAS, A. C. F.; MARRIEL, I. E.; ANDREOTE, F. D.; SELDIN, L.; ELSAS, J. D. van. Different effects of transgenic maize and nontransgenic maize on nitrogen-transforming archaea and bacteria in tropical soils. Applied and Environmental Microbiology, Washington, v. 80, n. 20, p. 6437-6445, Oct. 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 7. | | COTTA, S. R.; DIAS, A. C. F.; MARRIEL, I. E.; GOMES, E. A.; ELSAS, J. D. van; SELDIN, L. Temporal dynamics of microbial communities in the rhizosphere of two genetically modified (GM) maize hybrids in tropical agrosystems. Antonie van Leeuwenhoek, Dordrecht, v. 103, n. 3, p. 589-601, 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 8. | | EVANS, F. F.; ROSADO, A. S.; SEBASTIÁN, G. V.; CASELLA, R.; MACHADO, P. L. O. de A.; HOLMSTRÖM, C.; KJELLEBERG, S.; ELSAS, J. D. van; SELDIN, L. Impact of oil contamination and biostimulation on the diversity of indigenous bacterial communities in soil microcosms. FEMS Microbiology Ecology, v. 49, n. 2, p. 295-305, Aug. 2004. Na publicação: Pedro L. O. A. Machado.| Tipo: Artigo em Periódico Indexado |
| Biblioteca(s): Embrapa Solos. |
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| 9. | | OLIVEIRA, V. M. de; COUTINHO, H. L. da C.; SOBRAL, B. W. S.; GUIMARAES, C. T.; ELSAS, J. D. van; MANFIO, G. P. Discrimination of Rhizobium tropici and R. leguminosarum strains by PCR-specific amplification of 16S-23S rDNA spacer region fragments and denaturing gradient gel electrophoresis (DGGE). Letters in Applied Microbiology, v. 28, N. 2, p. 137-141, Feb. 1999.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Solos. |
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| 10. | | DIAS, A. C. F.; SILVA, M. C. P. e; SALLES, J. F.; AZEVEDO, J. L.; MELO, I. S. de; ELSAS, J. D. van; ANDREOTE, F. D. Linking the edges of microbially-mediated nitrogen transformations in mangrove sediments. In: SYMPOSIUM ON BACTERIAL GENETICS AND ECOLOGY - BAGECO 2011, 11., 2011, Corfu. Book of Abstracts... Corfu: University of Athens, 2011. P2.06.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 11. | | RACHID, C. T. C. C.; PICCOLO, M. C.; LEITE, D. C. A.; BALIEIRO, F. de C.; COUTINHO, H. L. da C.; ELSAS, J. D. van; PEIXOTO, R. S.; ROSADO, A. S. Physical-chemical and microbiological changes in Cerrado Soil under differing sugarcane harvest management systems. BMC Microbiology, v. 12, 170, 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Solos. |
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| 12. | | FASANELLA, C. C.; DIAS, A. C. F.; RIGONATO, J.; FIORE, M. F.; SOARES JUNIOR, F. L.; MELO, I. S. de; PIZZIRANI-KLEINER, A. A.; ELSAS, J. D. van; ANDREOTE, F. D. The selection exerted by oil contamination on mangrove fungal communities. Water, Air and Soil Pollution, Dordrecht, v. 223, n. 7, p. 4233-4243, 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 13. | | PEIXOTO, R. S.; COUTINHO, H. L. da C.; MADARI, B. E.; MACHADO, P. L. O. de A.; RUMJANEK, N. G.; ELSAS, J. D. van; SELDIN, L.; ROSADO, A. S. Soil aggregation and bacterial community structure as affected by tillage and cover cropping in the Brazilian cerrados. Soil & Tillage Research, Amsterdam, v. 90, n. 1/2, p. 16-28, Nov. 2006.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Agrobiologia; Embrapa Solos. |
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| Registros recuperados : 13 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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