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Registro Completo |
Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
11/09/2006 |
Data da última atualização: |
11/09/2006 |
Autoria: |
KOWALCHUCK, G. A.; BRUIJN, F. J. de; HEAD, I. M.; AKKERMANS, A. D. L.; ELSAS, J. D. van (Ed.). |
Título: |
Molecular microbial ecology manual. |
Edição: |
2. ed. |
Ano de publicação: |
2004 |
Fonte/Imprenta: |
Dordrecht: Kluwer, 2004. |
Volume: |
2v. |
Idioma: |
Inglês |
Conteúdo: |
Simplified protocols for the preparation of genomic DNA from bacterial cultures; Extraction of ribosomal RNA from microbial cultures; Extraction of microbial DNA from aquatic sources: marine environments; Extraction of microbial DNA from aquatic sources: freshwater; Methods for extracting DNA from microbial mats and cultivated micro-organisms: high molecular weight DNA from French press lysis; Extraction of microbial DNA from aquatic sediments; Extraction of microbial RNA from aquatic sources: marine environments; Extraction of total RNA and DNA from bacterioplankton; Methods for extracting RNA or ribosomes from microbial mats and cultivated microorganisms; Cell extraction method; DNA and RNA extraction from soil; Rapid simultaneous extraction of DNA and RNA from bulk and rhizosphere soil; Direct extraction of fungal DNA from soil; Purification of microbial genes from soil and rhizosphere by magnetic capture hybridization and subsequent amplification of target genes by PCR; Direct ribosome isolation from soil; DNA extraction from actinorhizal nodules; Quantification of nucleic acids; Quantification of nucleic acids from aquatic environments by using green-fluorescent dyes and microtiter plates; Degradation and turnover of extracellular DNA in marine sediments; Incorporation of thymidine into DNA of soil bacteria; Preparation of radioactive probes; Detection of nucleic ccids by chemiluminescence; Parameters of nucleic acid hybridization experiments; Detection and quantification of microbial DNA sequences in soil by Southern- and dot/slot blot hybridization; Detection of microbial DNA sequences by colony hybridization; Polymerase chain reaction analysis of soil microbial DNA; Detection of microbial nucleic acids by polymerase chain reaction in aquatic samples; Isolation and detection of bacterial DNA sequences in dairy products; Quantitative PCR of environmental samples; Molecular beacons for homogeneous real-time monitoring of amplification products; Detection and enumeration of soil bacteria using the MPN-PCR technique; Detection of mRNA and rRNA via reverse transcription and PCR in soil; Amplification of ribosomal RNA sequences; Cloning 16S rRNA genes and utilization to type bacterial communities; SARST, Serial Analysis of Ribosomal Sequence Tags; Oligonucleotide Fingerprinting of Ribosomal RNA Genes (OFRG); Genotyping of bacterial isolates from the environment using low-molecular-weight RNA fingerprints; Characterization of the diversity of ecologically important microbes by rep-PCR genomic fingerprinting; Genomic fingerprinting of micro-organisms by AFLPTM and ERIC-anchor PCR; The use of pulsed-field gel electrophoresis to study bacteria recovered from the environment; Easy individual strain and community typing by rDNA ITS1 analysis; In situ PCR methodologies for visualization of microscale genetic and taxonomic diversities of prokaryotic communities; Sensitive multi-color fluorescence in situ hybridization for the identification of environmental microorganisms; Use of Cloned Artificial Targets for FISH (catFISH) for the optimization of oligonucleotide probe hybridization conditions with 16S rRNA clones for in situ quantification of uncultivated prokaryotic cells; Denaturing gradient gel electrophoresis (DGGE) in microbial ecology; Fungal community analysis using PCR- Denaturing Gradient Gel Electrophoresis (DGGE); The Analysis of Microbial Communities with Terminal Restriction Fragment Length Polymorphism (T-RFLP) Microbial community analysis by PCR-single-strand conformation polymorphism (PCR-SSCP); Isolation of high molecular weight genomic DNA from soil bacteria for genomic library construction; Use of Biolog r _ for the Community Level Physiological Profiling (CLPP) of environmental samples; Fluorescent staining of microbes for total direct counts; Detection of microbes by Scanning Confocal Laser Microscopy (SCLM); Production of anti-microbial antibodies and their utilization in studies of microbial autecology by immunofluorescence microscopy and in situ CMEIAS image analysis; The slide immunoenzymatic assay (SIA): A simple and low cost system suitable for detecting water-borne microbes without the need for sophisticated technological infrastructure; In situ hybridization to detect microbial messenger RNA in plant tissues; Fatty acid analysis in the identification, taxonomy and ecology of (plant pathogenic) bacteria; Determination of microbial community structure using phospholipid fatty acid profiles; Respiratory lipoquinones as biomarkers; Environmental proteomics: methods and applications for aquatic ecosystems; Natural transformation in aquatic environments; Natural transformation in soil: microcosm studies; Plasmid transfer in aquatic environments; Conjugation in the epilithon; Detection of bacterial conjugation in soil; Transduction in the aquatic environment; Phage ecology and genetic exchange in soil; Lac as a marker gene to track microbes in the environment; XylE as a marker gene for microorganisms; GUS as a marker to track microbes; The celB marker gene; Visualisation of microbes and their interactions in the rhizosphere using auto fluorescent proteins as markers; Identification of bacteria by their intrinsic sequences: probe design and testing of their specificity; Subtraction hybridization for the production of high specificity DNA probes; Considerations for the use of functional markers and field release of genetically engineered microorganisms to soils and plants; Application of ecological diversity statistics in microbial ecology; Sampling efficiency and interpretation of diversity in 16S rRNA gene libraries; LIBSHUFF comparisons of 16S rRNA gene clone libraries; Cluster analysis and statistical comparison of molecular community profile data; Computer-assisted analysis of molecular fingerprint profiles and database construction; Multivariate statistical methods and artificial neural networks for analysis of microbial community molecular fingerprints; Quantitative fluorescence in situ hybridisation (FISH): statistical methods for valid cell counting; Oligonucleotide probe design for mixed microbial community microarrays and other applications and important considerations for data analysis; Design of microarrays for genome-wide expression profiling; Assessment of the membrane potential, intracellular pH and respiration of bacteria employing fluorescence techniques; Use of microelectrodes to measure in situ microbial activities in biofilms, sediments, and microbial mats; Application of whole-cell biosensors in soil; Detection of bacterial homoserine lactone quorum sensing signals; BrdU substrate utilization assay; Stable isotope probing of nucleic acids to identify active microbial populations; Linking microbial community structure and functioning: stable isotope (13C) labeling in combination with PLFA analysis; Correlating single-cell count with function in mixed natural microbial communities through STARFISH; Differential display of mRNA; Macro-arrays protocols for gene expression studies in bacteria; Oligonucleotide-based functional gene arrays for analysis of microbial communities in the environment; Proteomic analysis of bacterial systems. MenosSimplified protocols for the preparation of genomic DNA from bacterial cultures; Extraction of ribosomal RNA from microbial cultures; Extraction of microbial DNA from aquatic sources: marine environments; Extraction of microbial DNA from aquatic sources: freshwater; Methods for extracting DNA from microbial mats and cultivated micro-organisms: high molecular weight DNA from French press lysis; Extraction of microbial DNA from aquatic sediments; Extraction of microbial RNA from aquatic sources: marine environments; Extraction of total RNA and DNA from bacterioplankton; Methods for extracting RNA or ribosomes from microbial mats and cultivated microorganisms; Cell extraction method; DNA and RNA extraction from soil; Rapid simultaneous extraction of DNA and RNA from bulk and rhizosphere soil; Direct extraction of fungal DNA from soil; Purification of microbial genes from soil and rhizosphere by magnetic capture hybridization and subsequent amplification of target genes by PCR; Direct ribosome isolation from soil; DNA extraction from actinorhizal nodules; Quantification of nucleic acids; Quantification of nucleic acids from aquatic environments by using green-fluorescent dyes and microtiter plates; Degradation and turnover of extracellular DNA in marine sediments; Incorporation of thymidine into DNA of soil bacteria; Preparation of radioactive probes; Detection of nucleic ccids by chemiluminescence; Parameters of nucleic acid hybridization experiments; Detection and quantifi... Mostrar Tudo |
Thesagro: |
Bactéria; Microrganismo; Solo. |
Categoria do assunto: |
-- |
Marc: |
LEADER 07748nam a2200229 a 4500 001 1462634 005 2006-09-11 008 2004 bl uuuu 00u1 u #d 100 1 $aKOWALCHUCK, G. A. 245 $aMolecular microbial ecology manual. 250 $a2. ed. 260 $aDordrecht: Kluwer$c2004 300 $a2v. 490 $v2v. 520 $aSimplified protocols for the preparation of genomic DNA from bacterial cultures; Extraction of ribosomal RNA from microbial cultures; Extraction of microbial DNA from aquatic sources: marine environments; Extraction of microbial DNA from aquatic sources: freshwater; Methods for extracting DNA from microbial mats and cultivated micro-organisms: high molecular weight DNA from French press lysis; Extraction of microbial DNA from aquatic sediments; Extraction of microbial RNA from aquatic sources: marine environments; Extraction of total RNA and DNA from bacterioplankton; Methods for extracting RNA or ribosomes from microbial mats and cultivated microorganisms; Cell extraction method; DNA and RNA extraction from soil; Rapid simultaneous extraction of DNA and RNA from bulk and rhizosphere soil; Direct extraction of fungal DNA from soil; Purification of microbial genes from soil and rhizosphere by magnetic capture hybridization and subsequent amplification of target genes by PCR; Direct ribosome isolation from soil; DNA extraction from actinorhizal nodules; Quantification of nucleic acids; Quantification of nucleic acids from aquatic environments by using green-fluorescent dyes and microtiter plates; Degradation and turnover of extracellular DNA in marine sediments; Incorporation of thymidine into DNA of soil bacteria; Preparation of radioactive probes; Detection of nucleic ccids by chemiluminescence; Parameters of nucleic acid hybridization experiments; Detection and quantification of microbial DNA sequences in soil by Southern- and dot/slot blot hybridization; Detection of microbial DNA sequences by colony hybridization; Polymerase chain reaction analysis of soil microbial DNA; Detection of microbial nucleic acids by polymerase chain reaction in aquatic samples; Isolation and detection of bacterial DNA sequences in dairy products; Quantitative PCR of environmental samples; Molecular beacons for homogeneous real-time monitoring of amplification products; Detection and enumeration of soil bacteria using the MPN-PCR technique; Detection of mRNA and rRNA via reverse transcription and PCR in soil; Amplification of ribosomal RNA sequences; Cloning 16S rRNA genes and utilization to type bacterial communities; SARST, Serial Analysis of Ribosomal Sequence Tags; Oligonucleotide Fingerprinting of Ribosomal RNA Genes (OFRG); Genotyping of bacterial isolates from the environment using low-molecular-weight RNA fingerprints; Characterization of the diversity of ecologically important microbes by rep-PCR genomic fingerprinting; Genomic fingerprinting of micro-organisms by AFLPTM and ERIC-anchor PCR; The use of pulsed-field gel electrophoresis to study bacteria recovered from the environment; Easy individual strain and community typing by rDNA ITS1 analysis; In situ PCR methodologies for visualization of microscale genetic and taxonomic diversities of prokaryotic communities; Sensitive multi-color fluorescence in situ hybridization for the identification of environmental microorganisms; Use of Cloned Artificial Targets for FISH (catFISH) for the optimization of oligonucleotide probe hybridization conditions with 16S rRNA clones for in situ quantification of uncultivated prokaryotic cells; Denaturing gradient gel electrophoresis (DGGE) in microbial ecology; Fungal community analysis using PCR- Denaturing Gradient Gel Electrophoresis (DGGE); The Analysis of Microbial Communities with Terminal Restriction Fragment Length Polymorphism (T-RFLP) Microbial community analysis by PCR-single-strand conformation polymorphism (PCR-SSCP); Isolation of high molecular weight genomic DNA from soil bacteria for genomic library construction; Use of Biolog r _ for the Community Level Physiological Profiling (CLPP) of environmental samples; Fluorescent staining of microbes for total direct counts; Detection of microbes by Scanning Confocal Laser Microscopy (SCLM); Production of anti-microbial antibodies and their utilization in studies of microbial autecology by immunofluorescence microscopy and in situ CMEIAS image analysis; The slide immunoenzymatic assay (SIA): A simple and low cost system suitable for detecting water-borne microbes without the need for sophisticated technological infrastructure; In situ hybridization to detect microbial messenger RNA in plant tissues; Fatty acid analysis in the identification, taxonomy and ecology of (plant pathogenic) bacteria; Determination of microbial community structure using phospholipid fatty acid profiles; Respiratory lipoquinones as biomarkers; Environmental proteomics: methods and applications for aquatic ecosystems; Natural transformation in aquatic environments; Natural transformation in soil: microcosm studies; Plasmid transfer in aquatic environments; Conjugation in the epilithon; Detection of bacterial conjugation in soil; Transduction in the aquatic environment; Phage ecology and genetic exchange in soil; Lac as a marker gene to track microbes in the environment; XylE as a marker gene for microorganisms; GUS as a marker to track microbes; The celB marker gene; Visualisation of microbes and their interactions in the rhizosphere using auto fluorescent proteins as markers; Identification of bacteria by their intrinsic sequences: probe design and testing of their specificity; Subtraction hybridization for the production of high specificity DNA probes; Considerations for the use of functional markers and field release of genetically engineered microorganisms to soils and plants; Application of ecological diversity statistics in microbial ecology; Sampling efficiency and interpretation of diversity in 16S rRNA gene libraries; LIBSHUFF comparisons of 16S rRNA gene clone libraries; Cluster analysis and statistical comparison of molecular community profile data; Computer-assisted analysis of molecular fingerprint profiles and database construction; Multivariate statistical methods and artificial neural networks for analysis of microbial community molecular fingerprints; Quantitative fluorescence in situ hybridisation (FISH): statistical methods for valid cell counting; Oligonucleotide probe design for mixed microbial community microarrays and other applications and important considerations for data analysis; Design of microarrays for genome-wide expression profiling; Assessment of the membrane potential, intracellular pH and respiration of bacteria employing fluorescence techniques; Use of microelectrodes to measure in situ microbial activities in biofilms, sediments, and microbial mats; Application of whole-cell biosensors in soil; Detection of bacterial homoserine lactone quorum sensing signals; BrdU substrate utilization assay; Stable isotope probing of nucleic acids to identify active microbial populations; Linking microbial community structure and functioning: stable isotope (13C) labeling in combination with PLFA analysis; Correlating single-cell count with function in mixed natural microbial communities through STARFISH; Differential display of mRNA; Macro-arrays protocols for gene expression studies in bacteria; Oligonucleotide-based functional gene arrays for analysis of microbial communities in the environment; Proteomic analysis of bacterial systems. 650 $aBactéria 650 $aMicrorganismo 650 $aSolo 700 1 $aBRUIJN, F. J. de 700 1 $aHEAD, I. M. 700 1 $aAKKERMANS, A. D. L. 700 1 $aELSAS, J. D. van (Ed.).
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Registro Completo
Biblioteca(s): |
Embrapa Cerrados. |
Data corrente: |
18/05/2021 |
Data da última atualização: |
24/06/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 1 |
Autoria: |
NOCHI, A. R. F.; VARGAS, L. N.; SARTORI, R.; GUIMARAES JUNIOR, R.; ARAÚJO, D. B.; FIGUEIREDO, R. A.; TOGAWA, R. C.; COSTA, M. M.; GRYNBERG, P.; MENDONÇA, A. S.; KUSSANO, N. R.; PIVATO, I.; SILVA, B. D. M.; SPRICIGO, J. F. W.; LEME, L. O.; SILVA, J. P. da; CAETANO, A. R.; DODE, M. A. N.; FRANCO, M. M. |
Afiliação: |
ROBERTO GUIMARAES JUNIOR, CPAC; BIANCA DAMIANI MARQUES SILVA, Cenargen; JOSEANE PADILHA DA SILVA, Cenargen; ALEXANDRE RODRIGUES CAETANO, Cenargen; MARGOT ALVES NUNES DODE, Cenargen; MAURICIO MACHAIM FRANCO, Cenargen. |
Título: |
Low levels of sulfur and cobalt during the pre- and periconceptional periods affect the oocyte yield of donors and the DNA methylome of preimplantation bovine embryos. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Journal of Developmental Origins of Health and Disease, 2021. |
Páginas: |
13 p. |
Idioma: |
Inglês |
Conteúdo: |
Maternal nutrition is critical in mammalian development, influencing the epigenetic reprogramming of gametes, embryos, and fetal programming. We evaluated the effects of different levels of sulfur (S) and cobalt (Co) in the maternal diet throughout the pre- and periconceptional periods on the biochemical and reproductive parameters of the donors and the DNA methylome of the progeny in Bos indicus cattle. The low-S/Co group differed from the control with respect to homocysteine, folic acid, B12, insulin growth factor 1, and glucose. The oocyte yield was lower in heifers from the low S/Co group than that in the control heifers. Embryos from the low-S/Co group exhibited 2320 differentially methylated regions (DMRs) across the genome compared with the control embryos. We also characterized candidate DMRs linked to the DNMT1 and DNMT3B genes in the blood and sperm cells of the adult progeny. A DMR located in DNMT1 that was identified in embryos remained differentially methylated in the sperm of the progeny from the low-S/Co group. Therefore, we associated changes in specific compounds in the maternal diet with DNA methylation modifications in the progeny. Our results help to elucidate the impact of maternal nutrition on epigenetic reprogramming in livestock, opening new avenues of research to study the effect of disturbed epigenetic patterns in early life on health and fertility in adulthood. Considering that cattle are physiologically similar to humans with respect to gestational length, our study may serve as a model for studies related to the developmental origin of health and disease in humans. MenosMaternal nutrition is critical in mammalian development, influencing the epigenetic reprogramming of gametes, embryos, and fetal programming. We evaluated the effects of different levels of sulfur (S) and cobalt (Co) in the maternal diet throughout the pre- and periconceptional periods on the biochemical and reproductive parameters of the donors and the DNA methylome of the progeny in Bos indicus cattle. The low-S/Co group differed from the control with respect to homocysteine, folic acid, B12, insulin growth factor 1, and glucose. The oocyte yield was lower in heifers from the low S/Co group than that in the control heifers. Embryos from the low-S/Co group exhibited 2320 differentially methylated regions (DMRs) across the genome compared with the control embryos. We also characterized candidate DMRs linked to the DNMT1 and DNMT3B genes in the blood and sperm cells of the adult progeny. A DMR located in DNMT1 that was identified in embryos remained differentially methylated in the sperm of the progeny from the low-S/Co group. Therefore, we associated changes in specific compounds in the maternal diet with DNA methylation modifications in the progeny. Our results help to elucidate the impact of maternal nutrition on epigenetic reprogramming in livestock, opening new avenues of research to study the effect of disturbed epigenetic patterns in early life on health and fertility in adulthood. Considering that cattle are physiologically similar to humans with respect to gestationa... Mostrar Tudo |
Thesagro: |
Cobalto; Enxofre; Gado; Genética Animal; Nutrição Animal; Vaca. |
Thesaurus NAL: |
Epigenetics; Livestock. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02888naa a2200445 a 4500 001 2131856 005 2021-06-24 008 2021 bl uuuu u00u1 u #d 100 1 $aNOCHI, A. R. F. 245 $aLow levels of sulfur and cobalt during the pre- and periconceptional periods affect the oocyte yield of donors and the DNA methylome of preimplantation bovine embryos.$h[electronic resource] 260 $c2021 300 $a13 p. 520 $aMaternal nutrition is critical in mammalian development, influencing the epigenetic reprogramming of gametes, embryos, and fetal programming. We evaluated the effects of different levels of sulfur (S) and cobalt (Co) in the maternal diet throughout the pre- and periconceptional periods on the biochemical and reproductive parameters of the donors and the DNA methylome of the progeny in Bos indicus cattle. The low-S/Co group differed from the control with respect to homocysteine, folic acid, B12, insulin growth factor 1, and glucose. The oocyte yield was lower in heifers from the low S/Co group than that in the control heifers. Embryos from the low-S/Co group exhibited 2320 differentially methylated regions (DMRs) across the genome compared with the control embryos. We also characterized candidate DMRs linked to the DNMT1 and DNMT3B genes in the blood and sperm cells of the adult progeny. A DMR located in DNMT1 that was identified in embryos remained differentially methylated in the sperm of the progeny from the low-S/Co group. Therefore, we associated changes in specific compounds in the maternal diet with DNA methylation modifications in the progeny. Our results help to elucidate the impact of maternal nutrition on epigenetic reprogramming in livestock, opening new avenues of research to study the effect of disturbed epigenetic patterns in early life on health and fertility in adulthood. Considering that cattle are physiologically similar to humans with respect to gestational length, our study may serve as a model for studies related to the developmental origin of health and disease in humans. 650 $aEpigenetics 650 $aLivestock 650 $aCobalto 650 $aEnxofre 650 $aGado 650 $aGenética Animal 650 $aNutrição Animal 650 $aVaca 700 1 $aVARGAS, L. N. 700 1 $aSARTORI, R. 700 1 $aGUIMARAES JUNIOR, R. 700 1 $aARAÚJO, D. B. 700 1 $aFIGUEIREDO, R. A. 700 1 $aTOGAWA, R. C. 700 1 $aCOSTA, M. M. 700 1 $aGRYNBERG, P. 700 1 $aMENDONÇA, A. S. 700 1 $aKUSSANO, N. R. 700 1 $aPIVATO, I. 700 1 $aSILVA, B. D. M. 700 1 $aSPRICIGO, J. F. W. 700 1 $aLEME, L. O. 700 1 $aSILVA, J. P. da 700 1 $aCAETANO, A. R. 700 1 $aDODE, M. A. N. 700 1 $aFRANCO, M. M. 773 $tJournal of Developmental Origins of Health and Disease, 2021.
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