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Registro Completo |
Biblioteca(s): |
Embrapa Solos. |
Data corrente: |
19/10/2021 |
Data da última atualização: |
19/10/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
EVANS, F. F.; ROSADO, A. S.; SEBASTIÁN, G. V.; CASELLA, R.; MACHADO, P. L. O. de A.; HOLMSTRÖM, C.; KJELLEBERG, S.; ELSAS, J. D. van; SELDIN, L. |
Afiliação: |
FLAVIA F. EVANS, UFRJ; ALEXANDRE S. ROSADO, UFRJ; GINA V. SEBASTIÁN, CENPES/PETROBRAS; RENATA CASELLA, CENPES/PETROBRAS; PEDRO LUIZ OLIVEIRA DE A MACHADO, CNPS; CAROLA HOLMSTRÖM, UNIVERSITY OF NEW SOUTH WALES; STAFFAN KJELLEBERG, UNIVERSITY OF NEW SOUTH WALES; JAN D. VAN ELSAS, GRONINGEN UNIVERSITY; LUCY SELDIN, UFRJ. |
Título: |
Impact of oil contamination and biostimulation on the diversity of indigenous bacterial communities in soil microcosms. |
Ano de publicação: |
2004 |
Fonte/Imprenta: |
FEMS Microbiology Ecology, v. 49, n. 2, p. 295-305, Aug. 2004. |
DOI: |
https://doi.org/10.1016/j.femsec.2004.04.007 |
Idioma: |
Inglês |
Notas: |
Na publicação: Pedro L. O. A. Machado. |
Conteúdo: |
The aim of this study was to analyse the effect of oil contamination and biostimulation (soil pH raise, and nitrogen, phosphate and sulphur addition) on the diversity of a bacterial community of an acidic Cambisol under Atlantic Forest. The experiment was based on the enumeration of bacterial populations and hydrocarbon degraders in microcosms through the use of conventional plating techniques and molecular fingerprinting of samples directly from the environment. PCR followed by denaturing gradient gel electrophoresis (DGGE) was used to generate microbial community fingerprints employing 16S rRNA gene as molecular marker. Biostimulation led to increases of soil pH (to 7.0) and of the levels of phosphorus and K, Ca, and Mg. Oil contamination caused an increase in soil organic carbon (170-190% higher than control soil). Total bacterial counts were stable throughout the experiment, while MPN counts of hydrocarbon degraders showed an increase in the biostimulated and oil-contaminated soil samples. Molecular fingerprinting performed with 16S rRNA gene PCR and DGGE analysis revealed stable patterns along the 360 days of experiment, showing little change in oil-contaminated microcosms after 90 days. The DGGE patterns of the biostimulated samples showed severe changes due to decreases in the number of bands as compared to the control samples as from 15 days after addition of nutrients to the soil. Results obtained in the present study indicate that the addition of inorganic compounds to soil in conjunction with oil contamination has a greater impact on the bacterial community than oil contamination only. MenosThe aim of this study was to analyse the effect of oil contamination and biostimulation (soil pH raise, and nitrogen, phosphate and sulphur addition) on the diversity of a bacterial community of an acidic Cambisol under Atlantic Forest. The experiment was based on the enumeration of bacterial populations and hydrocarbon degraders in microcosms through the use of conventional plating techniques and molecular fingerprinting of samples directly from the environment. PCR followed by denaturing gradient gel electrophoresis (DGGE) was used to generate microbial community fingerprints employing 16S rRNA gene as molecular marker. Biostimulation led to increases of soil pH (to 7.0) and of the levels of phosphorus and K, Ca, and Mg. Oil contamination caused an increase in soil organic carbon (170-190% higher than control soil). Total bacterial counts were stable throughout the experiment, while MPN counts of hydrocarbon degraders showed an increase in the biostimulated and oil-contaminated soil samples. Molecular fingerprinting performed with 16S rRNA gene PCR and DGGE analysis revealed stable patterns along the 360 days of experiment, showing little change in oil-contaminated microcosms after 90 days. The DGGE patterns of the biostimulated samples showed severe changes due to decreases in the number of bands as compared to the control samples as from 15 days after addition of nutrients to the soil. Results obtained in the present study indicate that the addition of inorganic compound... Mostrar Tudo |
Palavras-Chave: |
16S rRNA gene; Atlantic forest; Bacterial diversity; Biostimulation; Crude oil; DGGE; Soil microcosms. |
Thesaurus Nal: |
Cambisols. |
Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/227068/1/Impact-of-oil-contamination-and-biostimulation-2004.pdf
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Marc: |
LEADER 02661naa a2200337 a 4500 001 2135450 005 2021-10-19 008 2004 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1016/j.femsec.2004.04.007$2DOI 100 1 $aEVANS, F. F. 245 $aImpact of oil contamination and biostimulation on the diversity of indigenous bacterial communities in soil microcosms.$h[electronic resource] 260 $c2004 500 $aNa publicação: Pedro L. O. A. Machado. 520 $aThe aim of this study was to analyse the effect of oil contamination and biostimulation (soil pH raise, and nitrogen, phosphate and sulphur addition) on the diversity of a bacterial community of an acidic Cambisol under Atlantic Forest. The experiment was based on the enumeration of bacterial populations and hydrocarbon degraders in microcosms through the use of conventional plating techniques and molecular fingerprinting of samples directly from the environment. PCR followed by denaturing gradient gel electrophoresis (DGGE) was used to generate microbial community fingerprints employing 16S rRNA gene as molecular marker. Biostimulation led to increases of soil pH (to 7.0) and of the levels of phosphorus and K, Ca, and Mg. Oil contamination caused an increase in soil organic carbon (170-190% higher than control soil). Total bacterial counts were stable throughout the experiment, while MPN counts of hydrocarbon degraders showed an increase in the biostimulated and oil-contaminated soil samples. Molecular fingerprinting performed with 16S rRNA gene PCR and DGGE analysis revealed stable patterns along the 360 days of experiment, showing little change in oil-contaminated microcosms after 90 days. The DGGE patterns of the biostimulated samples showed severe changes due to decreases in the number of bands as compared to the control samples as from 15 days after addition of nutrients to the soil. Results obtained in the present study indicate that the addition of inorganic compounds to soil in conjunction with oil contamination has a greater impact on the bacterial community than oil contamination only. 650 $aCambisols 653 $a16S rRNA gene 653 $aAtlantic forest 653 $aBacterial diversity 653 $aBiostimulation 653 $aCrude oil 653 $aDGGE 653 $aSoil microcosms 700 1 $aROSADO, A. S. 700 1 $aSEBASTIÁN, G. V. 700 1 $aCASELLA, R. 700 1 $aMACHADO, P. L. O. de A. 700 1 $aHOLMSTRÖM, C. 700 1 $aKJELLEBERG, S. 700 1 $aELSAS, J. D. van 700 1 $aSELDIN, L. 773 $tFEMS Microbiology Ecology$gv. 49, n. 2, p. 295-305, Aug. 2004.
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Registro original: |
Embrapa Solos (CNPS) |
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Registros recuperados : 13 | |
1. | | OLIVEIRA, V. M. de; COUTINHO, H. L. da C.; ELSAS, J. D. van; MANFIO, G. P. Avaliacao da diversidade de rizobios em amostras ambientais atraves do uso de primers especificos. In REUNIAO BRASILEIRA DE FERTILIDADE DE SOLO E NUTRICAO DE PLANTAS, 23.; REUNIAO BRASILEIRA SOBRE MICORRIZAS, 7.; SIMPOSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 5.; REUNIAO BRASILEIRA DE BIOLOGIA DO SOLO, 2., 1998, Caxambu,MG. Resumos... Lavras: UFLA / SBCS / SBM, 1998. p.808. FERTBIO 98: Interrelacao fertilidade, biologia de solo e nutricao de plantas: consolidacao de um paradigma.Biblioteca(s): Embrapa Solos. |
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2. | | WEID, I. von der; PAIVA, E.; NOBREGA, A.; ELSAS, J. D. van; SELDIN, L. Diversity of Paenibacillus polymyxa strains isolated from the rhizosphere of maize planted in cerrado soil. Research in Microbiology, Paris, v. 151, n. 5, p. 369-381, 2000.Tipo: Artigo em Periódico Indexado |
Biblioteca(s): Embrapa Milho e Sorgo. |
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3. | | COTTA, S. R.; DIAS, A. C. F.; MARRIEL, I. E.; GOMES, E. A.; ELSAS, J. D. van; SELDIN, L. Avaliação do possível impacto de milho geneticamente modificado na ciclagem do nitrogênio em solo de Cerrado. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 30.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 14.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 12.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 9.; SIMPÓSIO SOBRE SELÊNIO NO BRASIL, 1., 2012, Maceió. A responsabilidade socioambiental da pesquisa agrícola: anais. Viçosa, MG: Sociedade Brasileira de Ciência do Solo, 2012. 1 CD-ROM. Fertbio 2012.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Milho e Sorgo. |
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4. | | COTTA, S. R.; DIAS, A. C. F.; MARRIEL, I. E.; ANDREOTE, F. D.; SELDIN, L.; ELSAS, J. D. van. Different effects of transgenic maize and nontransgenic maize on nitrogen-transforming archaea and bacteria in tropical soils. Applied and Environmental Microbiology, Washington, v. 80, n. 20, p. 6437-6445, Oct. 2014.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Milho e Sorgo. |
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5. | | SELDIN, L.; ROSADO, A. S.; CRUZ, D. W. da; NOBREGA, A.; ELSAS, J. D. van; PAIVA, E. Comparison of Paenibacillus azotofixans strains isolated from rhizoplane, rhizosphere, and non-root-associated soil from maize planted in two different Brazilian soils. Applied and Environmental Microbiology, Washington, v. 64, n. 10, p. 3860-3868, 1998.Tipo: Artigo em Periódico Indexado |
Biblioteca(s): Embrapa Milho e Sorgo. |
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7. | | COTTA, S. R.; DIAS, A. C. F.; MARRIEL, I. E.; GOMES, E. A.; ELSAS, J. D. van; SELDIN, L. Temporal dynamics of microbial communities in the rhizosphere of two genetically modified (GM) maize hybrids in tropical agrosystems. Antonie van Leeuwenhoek, Dordrecht, v. 103, n. 3, p. 589-601, 2013.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
Biblioteca(s): Embrapa Milho e Sorgo. |
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8. | | OLIVEIRA, V. M. de; COUTINHO, H. L. da C.; SOBRAL, B. W. S.; GUIMARAES, C. T.; ELSAS, J. D. van; MANFIO, G. P. Discrimination of Rhizobium tropici and R. leguminosarum strains by PCR-specific amplification of 16S-23S rDNA spacer region fragments and denaturing gradient gel electrophoresis (DGGE). Letters in Applied Microbiology, v. 28, N. 2, p. 137-141, Feb. 1999.Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
Biblioteca(s): Embrapa Solos. |
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9. | | DIAS, A. C. F.; SILVA, M. C. P. e; SALLES, J. F.; AZEVEDO, J. L.; MELO, I. S. de; ELSAS, J. D. van; ANDREOTE, F. D. Linking the edges of microbially-mediated nitrogen transformations in mangrove sediments. In: SYMPOSIUM ON BACTERIAL GENETICS AND ECOLOGY - BAGECO 2011, 11., 2011, Corfu. Book of Abstracts... Corfu: University of Athens, 2011. P2.06.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Meio Ambiente. |
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10. | | EVANS, F. F.; ROSADO, A. S.; SEBASTIÁN, G. V.; CASELLA, R.; MACHADO, P. L. O. de A.; HOLMSTRÖM, C.; KJELLEBERG, S.; ELSAS, J. D. van; SELDIN, L. Impact of oil contamination and biostimulation on the diversity of indigenous bacterial communities in soil microcosms. FEMS Microbiology Ecology, v. 49, n. 2, p. 295-305, Aug. 2004. Na publicação: Pedro L. O. A. Machado.Tipo: Artigo em Periódico Indexado |
Biblioteca(s): Embrapa Solos. |
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11. | | RACHID, C. T. C. C.; PICCOLO, M. C.; LEITE, D. C. A.; BALIEIRO, F. de C.; COUTINHO, H. L. da C.; ELSAS, J. D. van; PEIXOTO, R. S.; ROSADO, A. S. Physical-chemical and microbiological changes in Cerrado Soil under differing sugarcane harvest management systems. BMC Microbiology, v. 12, 170, 2012.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Solos. |
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12. | | PEIXOTO, R. S.; COUTINHO, H. L. da C.; MADARI, B. E.; MACHADO, P. L. O. de A.; RUMJANEK, N. G.; ELSAS, J. D. van; SELDIN, L.; ROSADO, A. S. Soil aggregation and bacterial community structure as affected by tillage and cover cropping in the Brazilian cerrados. Soil & Tillage Research, Amsterdam, v. 90, n. 1/2, p. 16-28, Nov. 2006.Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
Biblioteca(s): Embrapa Agrobiologia; Embrapa Solos. |
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13. | | FASANELLA, C. C.; DIAS, A. C. F.; RIGONATO, J.; FIORE, M. F.; SOARES JUNIOR, F. L.; MELO, I. S. de; PIZZIRANI-KLEINER, A. A.; ELSAS, J. D. van; ANDREOTE, F. D. The selection exerted by oil contamination on mangrove fungal communities. Water, Air and Soil Pollution, Dordrecht, v. 223, n. 7, p. 4233-4243, 2012.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Meio Ambiente. |
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Registros recuperados : 13 | |
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Nenhum registro encontrado para a expressão de busca informada. |
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