Data/hora: 06/06/2024 02:39:48

Biblioteca(s): 	Embrapa Mandioca e Fruticultura.	
Data corrente: 	11/08/1999	
Data da última atualização: 	11/08/1999	
Autoria: 	ELIAS, M.; NAMBISAN, B. SUDHAKRAN, P. R.	
Afiliação: 	Department of Biochemistry, University of Kerala, Kariavattom, Trivandrum 695 581, India; and Central Tuber Crops Research Institute, Sreekaryam, Trivandrum 695 581, India.	
Título: 	Characterization of linamarase of latex and its localization in petioles in cassava	
Ano de publicação: 	1997	
Fonte/Imprenta: 	Archives of Biochemistry and Biophysics, v.341, n.2, p.222-228, 1997	
Idioma: 	Inglês	
Conteúdo: 	Yhe distribution of linamarase in latex and its purification, characterization, and immunocytochemical localization in petioles were studied in order to get an insight into the process of cyanogenesis in cassava. Crude latex exudate exhibited low linamarase activity, but on dilution the activity increased about fivefold. Assay using petiole latex collected in isotonic medium showed that the enzyme was distributed in vesicle-like structures. In vitro studies showed that about 50% activity was released from the vesicle into the medium within an hour. Latex linamarase was purified by ammonium sulfate fractionation and DEAE-cellulose chromatography and was characterized with respect to its amino acid composition and kinetic properties.	
Palavras-Chave: 	Linamarase; Localization; Petiole.	
Thesagro: 	Latex.	
Thesaurus Nal: 	cassava; cyanogenesis.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Mandioca e Fruticultura (CNPMF)	
Exemplares: 	CNPMF - (17264-1) - ADD - SP - -- - S11439 -  - S11439 - DIS