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Biblioteca(s): |
Embrapa Unidades Centrais. |
Data corrente: |
30/03/2016 |
Data da última atualização: |
30/03/2016 |
Autoria: |
BERNARDO, F. D.; CONHIZAK, C.; AMBROSINI, F.; JESUS, F. P. K. de; SANTURIO, J. M.; KOMMERS, G. D.; ELIAS, F.; FRANCISCATO, C. |
Afiliação: |
FÁBIO D. BERNARDO, UFFS; CLEVERSON CONHIZAK, Médico veterinário autônomo; FRANCIELLI AMBROSINI, UFFS; FRANCIELLI P. K. de JESUS, UFRGS; JANIO M. SANTURIO, UFSM; GLAUCIA D. KOMMERS, UFSM; FABIANA ELIAS, UFFS; CATARINA FRANCISCATO, UFFS. |
Título: |
Pythiosis in sheep from Paraná, southern Brazil. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Brasília, DF, v. 35, n. 6, p. 513-517, jun. 2015 |
Idioma: |
Inglês |
Conteúdo: |
This paper reports pythiosis in a sheep from southwestern Paraná, Brazil, confirmed by indirect ELISA (Enzime-Linked Immunosorbent Assay) and immunohistochemistry, as well as it describes the macro and microscopic injuries, in order to understand the pathogenicity. A 4-year-old ewe from a flock of 30 Santa Inês sheep, raised semi-extensively with access to a weir, showed cachexia, bilateral enlargement in nasal region, a serous and bloody secretion with a fetid odor from its nose and swollen submandibular and retropharyngeal lymph nodes. Blood collection was performed trough jugular vein puncture in order to make complete blood cell count (CBC) and to obtain serum for the subsequent serological examination. As the hematological counts were within the normal range for sheep, the animal was euthanized and submitted to necropsy. Indirect ELISA resulted positive for pythiosis. Necropsy revealed necrosis of the hard palate with a diameter of 3.5cm and extending up to the nasal cavity, forming a fistula. Submandibular and retropharyngeal lymph nodes were enlarged and edematous on section. Microscopic findings for submandibular and retropharyngeal lymph nodes consisted in moderate infiltration of eosinophils mainly in the subcapsular sinus, characterizing reactive eosinophilic lymphadenitis. The nasal cavity revealed rhinitis and oral cavity stomatitis with necro-eosinophilic and pronounced multifocal granulomatous infiltration and presence of hyphae. Hyphae found in palate and nasal cavity were positive for Pythium insidiosum by Grocott?s method and immunohistochemistry, the last one considered to be confirmatory for the pathogen diagnostic. This report has an important epidemiological aspect, as it is the first case of pythiosis in sheep confirmed by serology in South Brazil and an alert of possible infection by the pathogen in floodplains. MenosThis paper reports pythiosis in a sheep from southwestern Paraná, Brazil, confirmed by indirect ELISA (Enzime-Linked Immunosorbent Assay) and immunohistochemistry, as well as it describes the macro and microscopic injuries, in order to understand the pathogenicity. A 4-year-old ewe from a flock of 30 Santa Inês sheep, raised semi-extensively with access to a weir, showed cachexia, bilateral enlargement in nasal region, a serous and bloody secretion with a fetid odor from its nose and swollen submandibular and retropharyngeal lymph nodes. Blood collection was performed trough jugular vein puncture in order to make complete blood cell count (CBC) and to obtain serum for the subsequent serological examination. As the hematological counts were within the normal range for sheep, the animal was euthanized and submitted to necropsy. Indirect ELISA resulted positive for pythiosis. Necropsy revealed necrosis of the hard palate with a diameter of 3.5cm and extending up to the nasal cavity, forming a fistula. Submandibular and retropharyngeal lymph nodes were enlarged and edematous on section. Microscopic findings for submandibular and retropharyngeal lymph nodes consisted in moderate infiltration of eosinophils mainly in the subcapsular sinus, characterizing reactive eosinophilic lymphadenitis. The nasal cavity revealed rhinitis and oral cavity stomatitis with necro-eosinophilic and pronounced multifocal granulomatous infiltration and presence of hyphae. Hyphae found in palate and nas... Mostrar Tudo |
Palavras-Chave: |
Immunehistochemistry; Imuno-histoquímica. |
Thesagro: |
ELISA; Histopatologia; Ovino. |
Thesaurus Nal: |
Enzyme-linked immunosorbent assay; Pythium insidiosum. |
Categoria do assunto: |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/141834/1/Pythiosis-in-sheep.pdf
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Marc: |
LEADER 02675naa a2200289 a 4500 001 2042199 005 2016-03-30 008 2015 bl uuuu u00u1 u #d 100 1 $aBERNARDO, F. D. 245 $aPythiosis in sheep from Paraná, southern Brazil. 260 $c2015 520 $aThis paper reports pythiosis in a sheep from southwestern Paraná, Brazil, confirmed by indirect ELISA (Enzime-Linked Immunosorbent Assay) and immunohistochemistry, as well as it describes the macro and microscopic injuries, in order to understand the pathogenicity. A 4-year-old ewe from a flock of 30 Santa Inês sheep, raised semi-extensively with access to a weir, showed cachexia, bilateral enlargement in nasal region, a serous and bloody secretion with a fetid odor from its nose and swollen submandibular and retropharyngeal lymph nodes. Blood collection was performed trough jugular vein puncture in order to make complete blood cell count (CBC) and to obtain serum for the subsequent serological examination. As the hematological counts were within the normal range for sheep, the animal was euthanized and submitted to necropsy. Indirect ELISA resulted positive for pythiosis. Necropsy revealed necrosis of the hard palate with a diameter of 3.5cm and extending up to the nasal cavity, forming a fistula. Submandibular and retropharyngeal lymph nodes were enlarged and edematous on section. Microscopic findings for submandibular and retropharyngeal lymph nodes consisted in moderate infiltration of eosinophils mainly in the subcapsular sinus, characterizing reactive eosinophilic lymphadenitis. The nasal cavity revealed rhinitis and oral cavity stomatitis with necro-eosinophilic and pronounced multifocal granulomatous infiltration and presence of hyphae. Hyphae found in palate and nasal cavity were positive for Pythium insidiosum by Grocott?s method and immunohistochemistry, the last one considered to be confirmatory for the pathogen diagnostic. This report has an important epidemiological aspect, as it is the first case of pythiosis in sheep confirmed by serology in South Brazil and an alert of possible infection by the pathogen in floodplains. 650 $aEnzyme-linked immunosorbent assay 650 $aPythium insidiosum 650 $aELISA 650 $aHistopatologia 650 $aOvino 653 $aImmunehistochemistry 653 $aImuno-histoquímica 700 1 $aCONHIZAK, C. 700 1 $aAMBROSINI, F. 700 1 $aJESUS, F. P. K. de 700 1 $aSANTURIO, J. M. 700 1 $aKOMMERS, G. D. 700 1 $aELIAS, F. 700 1 $aFRANCISCATO, C. 773 $tPesquisa Veterinária Brasileira, Brasília, DF$gv. 35, n. 6, p. 513-517, jun. 2015
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Embrapa Unidades Centrais (AI-SEDE) |
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Registro Completo
Biblioteca(s): |
Embrapa Pantanal. |
Data corrente: |
16/01/2008 |
Data da última atualização: |
01/08/2019 |
Tipo da produção científica: |
Documentos |
Autoria: |
TOMICH, T. R.; PEREIRA, L. G. R.; GONÇALVES, L. C. |
Afiliação: |
Thierry Ribeiro Tomich, Embrapa Pantanal; Luiz Gustavo Ribeiro Pereira, UESC; Lúcio Carlos Gonçalves, UFMG. |
Título: |
Alimentos volumosos para o período seco - I: silagem de girassol. |
Ano de publicação: |
2004 |
Fonte/Imprenta: |
Corumbá: Embrapa Pantanal, 2004 |
Páginas: |
30 p. |
Série: |
(Embrapa Pantanal. Documentos, 72). |
Idioma: |
Português |
Notas: |
Formato Eletrônico |
Conteúdo: |
O objetivo desta publicação é reunir as principais informações relacionadas à silagem de girassol e, dessa forma, servir como embasamento para técnicos e produtores acerca da produção e conservação de uma opção de alimento volumoso de bom valor nutritivo. |
Palavras-Chave: |
Forragem conservada Helianthus annuus. |
Thesagro: |
Valor Nutritivo. |
Categoria do assunto: |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/CPAP/55979/1/DOC72.pdf
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Marc: |
LEADER 00833nam a2200193 a 4500 001 1784139 005 2019-08-01 008 2004 bl uuuu u0uu1 u #d 100 1 $aTOMICH, T. R. 245 $aAlimentos volumosos para o período seco - I$bsilagem de girassol. 260 $aCorumbá: Embrapa Pantanal$c2004 300 $a30 p. 490 $a(Embrapa Pantanal. Documentos, 72). 500 $aFormato Eletrônico 520 $aO objetivo desta publicação é reunir as principais informações relacionadas à silagem de girassol e, dessa forma, servir como embasamento para técnicos e produtores acerca da produção e conservação de uma opção de alimento volumoso de bom valor nutritivo. 650 $aValor Nutritivo 653 $aForragem conservada Helianthus annuus 700 1 $aPEREIRA, L. G. R. 700 1 $aGONÇALVES, L. C.
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