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Registro Completo |
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
31/05/2016 |
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Data da última atualização: |
29/12/2017 |
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Autoria: |
ELEOTÉRIO, R. B.; SEPÚLVEDA, R. V.; REIS, E. C. C.; VALENTE, F. L.; BORGES, A P. B. |
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Afiliação: |
RENATO B. ELEOTÉRIO, UFV; RODRIGO V. SEPULVEDA, UFV; EMILY C. C. REIS, UFV; FABRÍCIO L. VALENTE, UFV; ANDRÉA P. B. BORGES, UFV. |
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Título: |
Isolation, expansion and differentiation of mesenchymal stromal cells from rabbits' marrow'. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 36, n. 5, p. 423-430, maio. 2016. |
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Idioma: |
Inglês |
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Conteúdo: |
Tissue engineering has been a fundamental technique in the regenerative medicine field, once it permits to build tri-dimensional tissue constructs associating undifferentiated mesenchymal cells (or mesenchymal stromal cells - MSCs) and scaffolds in vitro. Therefore, many studies have been carried out using these cells from different animal species, and rabbits are often used as animal model for in vivo tissue repair studies. However, most of the information available about MSCs harvesting and characterization is about human and murine cells, which brings some doubts to researchers who desire to work with a rabbit model in tissue repair studies based on MSCs. In this context, this study aimed to add and improve the information available in the scientific literature providing a complete technique for isolation, expansion and differentiation of MSCs from rabbits. Bone marrow mononuclear cells (BMMCs) from humerus and femur of rabbits were obtained and to evaluate their proliferation rate, three different culture media were tested, here referred as DMEM-P, DMEM´S and ?-MEM. The BMMCs were also cultured in osteogenic, chondrogenic and adipogenic induction media to prove their multipotentiality. It was concluded that the techniques suggested in this study can provide a guideline to harvest and isolate MSCs from bone marrow of rabbits in enough amount to allow their expansion and, based on the laboratory experience where the study was developed, it is also suggested a culture media formulation to provide a better cell proliferation rate with multipotentiality preservation. MenosTissue engineering has been a fundamental technique in the regenerative medicine field, once it permits to build tri-dimensional tissue constructs associating undifferentiated mesenchymal cells (or mesenchymal stromal cells - MSCs) and scaffolds in vitro. Therefore, many studies have been carried out using these cells from different animal species, and rabbits are often used as animal model for in vivo tissue repair studies. However, most of the information available about MSCs harvesting and characterization is about human and murine cells, which brings some doubts to researchers who desire to work with a rabbit model in tissue repair studies based on MSCs. In this context, this study aimed to add and improve the information available in the scientific literature providing a complete technique for isolation, expansion and differentiation of MSCs from rabbits. Bone marrow mononuclear cells (BMMCs) from humerus and femur of rabbits were obtained and to evaluate their proliferation rate, three different culture media were tested, here referred as DMEM-P, DMEM´S and ?-MEM. The BMMCs were also cultured in osteogenic, chondrogenic and adipogenic induction media to prove their multipotentiality. It was concluded that the techniques suggested in this study can provide a guideline to harvest and isolate MSCs from bone marrow of rabbits in enough amount to allow their expansion and, based on the laboratory experience where the study was developed, it is also suggested a culture media... Mostrar Tudo |
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Palavras-Chave: |
Bone marrow stem cells; Cell therapy; Células tronco mesenquimais; Engenharia de tecido; Medicina regenerativa; Mesenchymal stromal cells; Regenerative medicine; Terapia celular. |
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Thesagro: |
Coelho; Medula osséa. |
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Thesaurus Nal: |
Rabbits; Tissue engineering. |
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Categoria do assunto: |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/143602/1/Isolation-expansion.pdf
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Marc: |
LEADER 02539naa a2200313 a 4500
001 2045981
005 2017-12-29
008 2016 bl uuuu u00u1 u #d
100 1 $aELEOTÉRIO, R. B.
245 $aIsolation, expansion and differentiation of mesenchymal stromal cells from rabbits' marrow'.
260 $c2016
520 $aTissue engineering has been a fundamental technique in the regenerative medicine field, once it permits to build tri-dimensional tissue constructs associating undifferentiated mesenchymal cells (or mesenchymal stromal cells - MSCs) and scaffolds in vitro. Therefore, many studies have been carried out using these cells from different animal species, and rabbits are often used as animal model for in vivo tissue repair studies. However, most of the information available about MSCs harvesting and characterization is about human and murine cells, which brings some doubts to researchers who desire to work with a rabbit model in tissue repair studies based on MSCs. In this context, this study aimed to add and improve the information available in the scientific literature providing a complete technique for isolation, expansion and differentiation of MSCs from rabbits. Bone marrow mononuclear cells (BMMCs) from humerus and femur of rabbits were obtained and to evaluate their proliferation rate, three different culture media were tested, here referred as DMEM-P, DMEM´S and ?-MEM. The BMMCs were also cultured in osteogenic, chondrogenic and adipogenic induction media to prove their multipotentiality. It was concluded that the techniques suggested in this study can provide a guideline to harvest and isolate MSCs from bone marrow of rabbits in enough amount to allow their expansion and, based on the laboratory experience where the study was developed, it is also suggested a culture media formulation to provide a better cell proliferation rate with multipotentiality preservation.
650 $aRabbits
650 $aTissue engineering
650 $aCoelho
650 $aMedula osséa
653 $aBone marrow stem cells
653 $aCell therapy
653 $aCélulas tronco mesenquimais
653 $aEngenharia de tecido
653 $aMedicina regenerativa
653 $aMesenchymal stromal cells
653 $aRegenerative medicine
653 $aTerapia celular
700 1 $aSEPÚLVEDA, R. V.
700 1 $aREIS, E. C. C.
700 1 $aVALENTE, F. L.
700 1 $aBORGES, A P. B.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 36, n. 5, p. 423-430, maio. 2016.
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Embrapa Unidades Centrais (AI-SEDE) |
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Registro Completo
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
03/01/2012 |
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Data da última atualização: |
10/08/2017 |
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Tipo da produção científica: |
Orientação de Tese de Pós-Graduação |
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Autoria: |
PINTO, P. H. N. |
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Afiliação: |
Pedro Henrique Nicolau Pinto. |
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Título: |
Uso de sêmen resfriado e inseminação artificial em caprinos leiteiros na República de Cabo Verde. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
2011. |
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Páginas: |
88 f. |
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Idioma: |
Português |
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Notas: |
Dissertação (Mestrado em Ciência Veterinárias) - Universidade Federal do Paraná, Curitiba. Orientador: José Antônio de Freitas; Co-orientador: Jeferson Ferreira da Fonseca, Embrapa Caprinos e Ovinos (CNPC). |
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Conteúdo: |
Resumo: Resumo: Este projeto e fruto de um trabalho conjunto entre a Agencia Brasileira de Cooperacao Internacional, CNPq, Embrapa, Governo de Cabo Verde, GOPA, UFF e UFPR. Teve como objetivo treinar tecnicos caboverdianos, para que possam executar todas as etapas envolvidas em um programa de inseminacao artificial (IA); determinar um protocolo de IA com semen resfriado que possibilite a disseminacao de genetica para todas as ilhas do arquipelago; fazer uma primeira disseminacao genetica; estruturar um centro de coleta e manipulacao de semen caprino; alem de avaliar a viabilidade da utilizacao do semen caprino diluido em meio tris-gema 2,5% (Evans & Maxwell, 1987; modificado), resfriado a 5 ‹C e armazenado por diferentes periodos (24 ou 48 horas). Foram inseminadas por via transcervical 133 cabras sem raca definida e nativas da Republica de Cabo Verde, divididas aleatoriamente em dois tratamentos T24 e T48. O estro foi sincronizado com a utilizacao de esponjas intra-vaginais contendo 60 mg de acetato de medroxiprogesterona por seis dias; 37,5 ƒÊg de D-cloprostenol e 200 UI de eCG 24 horas antes da retirada da esponja. Foram utilizados tres reprodutores da Raca Canarias, foi utilizado dose inseminante de 150 x 106 de espermatozoides viaveis. Para resfriar e manter o semen a 5o C foi utilizado o BotutainerR (Biotech Botucatu, Reproducao Animal, Botucatu - SP) adaptado. As analises estatisticas foram realizadas utilizando-se o pacote computacional SAEG (2009). Nao houve diferenca (P>0,05) entre os padroes seminais para os diferentes periodos de resfriamento (T24 - 58,8% }11,1 de motilidade e 2,9 }0,5 de vigor; T48 - 51,3% }2,5 de motilidade e 2,8 }0,3 de vigor), o que permitiu obter taxas de paricao similares em ambos os tratamentos (T24-26,5% e T48-21,5%). A eficiencia dos protocolos testados permitiu a disseminacao de genetica caprina na Republica de Cabo Verde. Houve correlacao (r = 0,27; P<0,05) entre o intervalo de retirada do progestageno a inseminacao artificial (IRIA) com a profundidade de deposicao de semen (PROF). Houve tambem correlacao (r = 0,29; P<0,05) entre o IRIA e a taxa de paricao (PARI). Conclui-se que o semen caprino, resfriado por 48 horas a 50C, apresenta o mesmo potencial de fertilizacao do semen resfriado por 24 horas a 50C. Abstract: This project is the result of a collaborative effort among the Brazilian Agency for International Cooperation, the National Council of Scientific and Technological Development (CNPq), Brazilian Agricultural Research Corporation – Embrapa Goats and Sheep, the government of Cape Verde, GOPA (Worldwide Consultants), the Federal University of Rio de Janeiro (UFF) and the Federal University of Paraná (UFPR). It aimed to train Cape Verdeans technicians to perform all the steps involved in a program for goat artificial insemination (AI); to determine a protocol for AI with chilled semen that enables the dissemination of genetics to all islands of the archipelago; to make a first genetic spread; and to structure a center for collection and handling goat semen. The fertilizing capacity of goat semen diluted in tris-egg yolk 2.5% (Evans & Maxwell, 1987; modified), chilled at 5 oC for 24 or 48 hours was evaluated. Transcervical artificial insemination was performed in 133 goats that were divided, randomly, into two treatments T24 and T48. The estrus was synchronized by the mean of intravaginal sponges containing 60 mg of medroxyprogesterone acetate for six days; 37.5 mg of D-cloprostenol and 200 IU eCG 24 hours before removing the sponge. Three Canarian’s Buck were used, the insemination dose was 150 x 106 mobile spermatozoa, for cooling and keeping the semen at 5 oC a Botutainer® (Biotech Botucatu, Animal Reproduction, Botucatu - SP) was adapted and used. There was no difference (P> 0.05) between the seminal patterns for the different periods of cooling (T24 - 58.8%±11.1 for motility and 2.9±0.5 for strength; T48 - 51.3%±2.5 for motility and 2.8±0.3 for strength), which allowed to obtain similar pregnancy rates in both treatments (T24 – 26.5% and T48 - 21,5%). The efficiency of the tested protocols allowed the dissemination of goat’s genetic material in the Republic of Cape Verde. There was a correlation (r = 0.27, P < 0.05) between the range of sponge withdrawal to artificial insemination (IRIA) with the depth of semen deposition (PROF). There was also a correlation (r = 0.29, P < 0.05) between IRIA and the calving rate (PARI). Statistical analysis was done in the computer package SAEG (2009). It was concluded that goat semen, cooled for 48 hours at 5 oC, has the same fertility that semen cooled for 24 hours at 5 oC. MenosResumo: Resumo: Este projeto e fruto de um trabalho conjunto entre a Agencia Brasileira de Cooperacao Internacional, CNPq, Embrapa, Governo de Cabo Verde, GOPA, UFF e UFPR. Teve como objetivo treinar tecnicos caboverdianos, para que possam executar todas as etapas envolvidas em um programa de inseminacao artificial (IA); determinar um protocolo de IA com semen resfriado que possibilite a disseminacao de genetica para todas as ilhas do arquipelago; fazer uma primeira disseminacao genetica; estruturar um centro de coleta e manipulacao de semen caprino; alem de avaliar a viabilidade da utilizacao do semen caprino diluido em meio tris-gema 2,5% (Evans & Maxwell, 1987; modificado), resfriado a 5 ‹C e armazenado por diferentes periodos (24 ou 48 horas). Foram inseminadas por via transcervical 133 cabras sem raca definida e nativas da Republica de Cabo Verde, divididas aleatoriamente em dois tratamentos T24 e T48. O estro foi sincronizado com a utilizacao de esponjas intra-vaginais contendo 60 mg de acetato de medroxiprogesterona por seis dias; 37,5 ƒÊg de D-cloprostenol e 200 UI de eCG 24 horas antes da retirada da esponja. Foram utilizados tres reprodutores da Raca Canarias, foi utilizado dose inseminante de 150 x 106 de espermatozoides viaveis. Para resfriar e manter o semen a 5o C foi utilizado o BotutainerR (Biotech Botucatu, Reproducao Animal, Botucatu - SP) adaptado. As analises estatisticas foram realizadas utilizando-se o pacote computacional SAEG (2009). Nao houve diferen... Mostrar Tudo |
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Thesagro: |
Caprino; Inseminação artificial; Reprodução animal. |
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Thesaurus NAL: |
Goats; Reproduction. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/96065/1/TS-Uso-do-semen.pdf
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Marc: |
LEADER 05396nam a2200193 a 4500
001 1911456
005 2017-08-10
008 2011 bl uuuu m 00u1 u #d
100 1 $aPINTO, P. H. N.
245 $aUso de sêmen resfriado e inseminação artificial em caprinos leiteiros na República de Cabo Verde.
260 $a2011.$c2011
300 $a88 f.
500 $aDissertação (Mestrado em Ciência Veterinárias) - Universidade Federal do Paraná, Curitiba. Orientador: José Antônio de Freitas; Co-orientador: Jeferson Ferreira da Fonseca, Embrapa Caprinos e Ovinos (CNPC).
520 $aResumo: Resumo: Este projeto e fruto de um trabalho conjunto entre a Agencia Brasileira de Cooperacao Internacional, CNPq, Embrapa, Governo de Cabo Verde, GOPA, UFF e UFPR. Teve como objetivo treinar tecnicos caboverdianos, para que possam executar todas as etapas envolvidas em um programa de inseminacao artificial (IA); determinar um protocolo de IA com semen resfriado que possibilite a disseminacao de genetica para todas as ilhas do arquipelago; fazer uma primeira disseminacao genetica; estruturar um centro de coleta e manipulacao de semen caprino; alem de avaliar a viabilidade da utilizacao do semen caprino diluido em meio tris-gema 2,5% (Evans & Maxwell, 1987; modificado), resfriado a 5 ‹C e armazenado por diferentes periodos (24 ou 48 horas). Foram inseminadas por via transcervical 133 cabras sem raca definida e nativas da Republica de Cabo Verde, divididas aleatoriamente em dois tratamentos T24 e T48. O estro foi sincronizado com a utilizacao de esponjas intra-vaginais contendo 60 mg de acetato de medroxiprogesterona por seis dias; 37,5 ƒÊg de D-cloprostenol e 200 UI de eCG 24 horas antes da retirada da esponja. Foram utilizados tres reprodutores da Raca Canarias, foi utilizado dose inseminante de 150 x 106 de espermatozoides viaveis. Para resfriar e manter o semen a 5o C foi utilizado o BotutainerR (Biotech Botucatu, Reproducao Animal, Botucatu - SP) adaptado. As analises estatisticas foram realizadas utilizando-se o pacote computacional SAEG (2009). Nao houve diferenca (P>0,05) entre os padroes seminais para os diferentes periodos de resfriamento (T24 - 58,8% }11,1 de motilidade e 2,9 }0,5 de vigor; T48 - 51,3% }2,5 de motilidade e 2,8 }0,3 de vigor), o que permitiu obter taxas de paricao similares em ambos os tratamentos (T24-26,5% e T48-21,5%). A eficiencia dos protocolos testados permitiu a disseminacao de genetica caprina na Republica de Cabo Verde. Houve correlacao (r = 0,27; P<0,05) entre o intervalo de retirada do progestageno a inseminacao artificial (IRIA) com a profundidade de deposicao de semen (PROF). Houve tambem correlacao (r = 0,29; P<0,05) entre o IRIA e a taxa de paricao (PARI). Conclui-se que o semen caprino, resfriado por 48 horas a 50C, apresenta o mesmo potencial de fertilizacao do semen resfriado por 24 horas a 50C. Abstract: This project is the result of a collaborative effort among the Brazilian Agency for International Cooperation, the National Council of Scientific and Technological Development (CNPq), Brazilian Agricultural Research Corporation – Embrapa Goats and Sheep, the government of Cape Verde, GOPA (Worldwide Consultants), the Federal University of Rio de Janeiro (UFF) and the Federal University of Paraná (UFPR). It aimed to train Cape Verdeans technicians to perform all the steps involved in a program for goat artificial insemination (AI); to determine a protocol for AI with chilled semen that enables the dissemination of genetics to all islands of the archipelago; to make a first genetic spread; and to structure a center for collection and handling goat semen. The fertilizing capacity of goat semen diluted in tris-egg yolk 2.5% (Evans & Maxwell, 1987; modified), chilled at 5 oC for 24 or 48 hours was evaluated. Transcervical artificial insemination was performed in 133 goats that were divided, randomly, into two treatments T24 and T48. The estrus was synchronized by the mean of intravaginal sponges containing 60 mg of medroxyprogesterone acetate for six days; 37.5 mg of D-cloprostenol and 200 IU eCG 24 hours before removing the sponge. Three Canarian’s Buck were used, the insemination dose was 150 x 106 mobile spermatozoa, for cooling and keeping the semen at 5 oC a Botutainer® (Biotech Botucatu, Animal Reproduction, Botucatu - SP) was adapted and used. There was no difference (P> 0.05) between the seminal patterns for the different periods of cooling (T24 - 58.8%±11.1 for motility and 2.9±0.5 for strength; T48 - 51.3%±2.5 for motility and 2.8±0.3 for strength), which allowed to obtain similar pregnancy rates in both treatments (T24 – 26.5% and T48 - 21,5%). The efficiency of the tested protocols allowed the dissemination of goat’s genetic material in the Republic of Cape Verde. There was a correlation (r = 0.27, P < 0.05) between the range of sponge withdrawal to artificial insemination (IRIA) with the depth of semen deposition (PROF). There was also a correlation (r = 0.29, P < 0.05) between IRIA and the calving rate (PARI). Statistical analysis was done in the computer package SAEG (2009). It was concluded that goat semen, cooled for 48 hours at 5 oC, has the same fertility that semen cooled for 24 hours at 5 oC.
650 $aGoats
650 $aReproduction
650 $aCaprino
650 $aInseminação artificial
650 $aReprodução animal
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Embrapa Caprinos e Ovinos (CNPC) |
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