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 | Acesso ao texto completo restrito à biblioteca da Embrapa Café. Para informações adicionais entre em contato com biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
04/03/2011 |
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Data da última atualização: |
04/03/2011 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
SATO, J. H.; TEIXEIRA, J. B.; EIRA, M. T. S. da; FRANCO, O. L.; REIS, A. M. dos. |
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Afiliação: |
UNB; JOAO BATISTA TEIXEIRA, CENARGEN; MIRIAN THEREZINHA SOUZA DA EIRA, SAPC; UNIVERSIDADE CATÓLICA DE BRASÍLIA; ANGELA MEHTA DOS REIS, CENARGEN. |
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Título: |
Comparative proteomic analysis of Coffea arabica somatic embryos in the different stages of embryogenesis. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 22., 2008, Campinas. |
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Idioma: |
Inglês |
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Conteúdo: |
Coffee is one of the most important cultivated agricultural crops and is of great importance for Brazilian economy. Studies focusing on somatic embryogenesis have been widely performed aiming at the comprehension of the mechanisms involved. Somatic embryogenesis is an important process that helps in vitro micropropagation, which is performed to obtain a high number of plants with high economic value, genetically uniform and free from diseases. The objective of this study was to analyze the protein profile of Coffea arabica cv. Catuaí Vermelho somatic embryos in the stages of torpedo and cotyledonary by two-dimensional electrophoresis (2-DE). Samples were collected and macerated in liquid nitrogen. Protein extraction was performed by using phenol and extraction buffer. The proteins obtained were quantified, analyzed by 2-DE and stained with silver nitrate. The 2D gels revealed proteins varying in size from 10 to 160 kDa and in pI from 3 to 10. The analysis of the protein profiles showed a higher amount of proteins in the cotyledonary phase, especially in the area of pH 3 to 5,5. A total of 45 differentially expressed proteins were observed when comparing both profiles, including 8 proteins observed only in the cotyledonary phase and 4 exclusive to the torpedo phase. Theses proteins will be further identified by mass spectrometry in order to better understand the expression changes during somatic embryogenesis in coffee. |
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Palavras-Chave: |
Coffee; In vitro micropropagation. |
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Thesaurus Nal: |
Somatic embryogenesis. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02078nam a2200193 a 4500
001 1880074
005 2011-03-04
008 2008 bl uuuu u00u1 u #d
100 1 $aSATO, J. H.
245 $aComparative proteomic analysis of Coffea arabica somatic embryos in the different stages of embryogenesis.$h[electronic resource]
260 $aIn: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 22., 2008, Campinas.$c2008
520 $aCoffee is one of the most important cultivated agricultural crops and is of great importance for Brazilian economy. Studies focusing on somatic embryogenesis have been widely performed aiming at the comprehension of the mechanisms involved. Somatic embryogenesis is an important process that helps in vitro micropropagation, which is performed to obtain a high number of plants with high economic value, genetically uniform and free from diseases. The objective of this study was to analyze the protein profile of Coffea arabica cv. Catuaí Vermelho somatic embryos in the stages of torpedo and cotyledonary by two-dimensional electrophoresis (2-DE). Samples were collected and macerated in liquid nitrogen. Protein extraction was performed by using phenol and extraction buffer. The proteins obtained were quantified, analyzed by 2-DE and stained with silver nitrate. The 2D gels revealed proteins varying in size from 10 to 160 kDa and in pI from 3 to 10. The analysis of the protein profiles showed a higher amount of proteins in the cotyledonary phase, especially in the area of pH 3 to 5,5. A total of 45 differentially expressed proteins were observed when comparing both profiles, including 8 proteins observed only in the cotyledonary phase and 4 exclusive to the torpedo phase. Theses proteins will be further identified by mass spectrometry in order to better understand the expression changes during somatic embryogenesis in coffee.
650 $aSomatic embryogenesis
653 $aCoffee
653 $aIn vitro micropropagation
700 1 $aTEIXEIRA, J. B.
700 1 $aEIRA, M. T. S. da
700 1 $aFRANCO, O. L.
700 1 $aREIS, A. M. dos
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Registro original: |
Embrapa Café (CNPCa) |
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Registro Completo
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
07/04/2015 |
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Data da última atualização: |
08/04/2015 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
BERTECHINI, A. G.; MAZZUCO, H.; RODRIGUES, E. C.; RAMOS, E. M. |
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Afiliação: |
ANTONIO GILBERTO BERTECHINI, UFL; HELENICE MAZZUCO, CNPSA; ERIKA CRISTINA RODRIGUES; EDUARDO MENDES RAMOS, UFL. |
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Título: |
Study of the utilization of light egg-type males: A proposal for the sustainability of the egg industry |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Poultry Science, v. 93, p. 755-761, 2014. |
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DOI: |
10.3382/ps.2013-03462 |
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Idioma: |
Inglês |
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Conteúdo: |
This study evaluated the performance and viability of light egg-type males, usually euthanized at the hatcheries, from White and Brown Leghorn laying hen lines. One-day-old male chicks, half from each hen line, were raised in floor pens until they were 42 d of age. The birds were distributed into 48 floor pens, furnished with tube feeders and nipple drinkers, and submitted to 24 h of continuous light, 3 feeding phases (1-7, 8-21, and 22-42 d) and diets composed of corn and soybean meal as the main ingredients. A completely randomized design was used in a 4 × 2 factorial arrangement (phase levels of ME dietary treatments × strain) of 6 replicates of 40 birds each. The variables evaluated were bird performance, carcass quality, and yield at 42 d of age. Mortality and cannibalism were not observed during the entire experimental period, although the birds' beaks were not trimmed. The brown males line showed higher feed consumption and BW gain and better feed conversion compared with the white male line (P< 0.05). Metabolizable energy (kcal/kg) levels of 3,200 (1 to 7 d), 3,050 (8 to 21 d), and 3,200 (22 to 42 d) provided better performance (P< 0.05) in both lines. The carcass yields were similar (P> 0.05) between the 2 lines; males from the white line showed higher breast yield, and the brown line males showed higher yield of thighs and drumstick (P< 0.05). The treatments had no effect on meat quality (P > 0.05). Overall, the results suggest that there is great viability for the use of male chicks from laying hens of both leghorn lines as a high quality protein source for human consumption. MenosThis study evaluated the performance and viability of light egg-type males, usually euthanized at the hatcheries, from White and Brown Leghorn laying hen lines. One-day-old male chicks, half from each hen line, were raised in floor pens until they were 42 d of age. The birds were distributed into 48 floor pens, furnished with tube feeders and nipple drinkers, and submitted to 24 h of continuous light, 3 feeding phases (1-7, 8-21, and 22-42 d) and diets composed of corn and soybean meal as the main ingredients. A completely randomized design was used in a 4 × 2 factorial arrangement (phase levels of ME dietary treatments × strain) of 6 replicates of 40 birds each. The variables evaluated were bird performance, carcass quality, and yield at 42 d of age. Mortality and cannibalism were not observed during the entire experimental period, although the birds' beaks were not trimmed. The brown males line showed higher feed consumption and BW gain and better feed conversion compared with the white male line (P< 0.05). Metabolizable energy (kcal/kg) levels of 3,200 (1 to 7 d), 3,050 (8 to 21 d), and 3,200 (22 to 42 d) provided better performance (P< 0.05) in both lines. The carcass yields were similar (P> 0.05) between the 2 lines; males from the white line showed higher breast yield, and the brown line males showed higher yield of thighs and drumstick (P< 0.05). The treatments had no effect on meat quality (P > 0.05). Overall, the results suggest that there is great viability for the... Mostrar Tudo |
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Palavras-Chave: |
Qualidade da carne; Qualidade do ovo; Sustentabilidade. |
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Thesagro: |
Industrialização. |
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Thesaurus NAL: |
Egg industry; Egg quality; Environmental sustainability; Meat quality. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02420naa a2200265 a 4500
001 2012932
005 2015-04-08
008 2014 bl uuuu u00u1 u #d
024 7 $a10.3382/ps.2013-03462$2DOI
100 1 $aBERTECHINI, A. G.
245 $aStudy of the utilization of light egg-type males$bA proposal for the sustainability of the egg industry$h[electronic resource]
260 $c2014
520 $aThis study evaluated the performance and viability of light egg-type males, usually euthanized at the hatcheries, from White and Brown Leghorn laying hen lines. One-day-old male chicks, half from each hen line, were raised in floor pens until they were 42 d of age. The birds were distributed into 48 floor pens, furnished with tube feeders and nipple drinkers, and submitted to 24 h of continuous light, 3 feeding phases (1-7, 8-21, and 22-42 d) and diets composed of corn and soybean meal as the main ingredients. A completely randomized design was used in a 4 × 2 factorial arrangement (phase levels of ME dietary treatments × strain) of 6 replicates of 40 birds each. The variables evaluated were bird performance, carcass quality, and yield at 42 d of age. Mortality and cannibalism were not observed during the entire experimental period, although the birds' beaks were not trimmed. The brown males line showed higher feed consumption and BW gain and better feed conversion compared with the white male line (P< 0.05). Metabolizable energy (kcal/kg) levels of 3,200 (1 to 7 d), 3,050 (8 to 21 d), and 3,200 (22 to 42 d) provided better performance (P< 0.05) in both lines. The carcass yields were similar (P> 0.05) between the 2 lines; males from the white line showed higher breast yield, and the brown line males showed higher yield of thighs and drumstick (P< 0.05). The treatments had no effect on meat quality (P > 0.05). Overall, the results suggest that there is great viability for the use of male chicks from laying hens of both leghorn lines as a high quality protein source for human consumption.
650 $aEgg industry
650 $aEgg quality
650 $aEnvironmental sustainability
650 $aMeat quality
650 $aIndustrialização
653 $aQualidade da carne
653 $aQualidade do ovo
653 $aSustentabilidade
700 1 $aMAZZUCO, H.
700 1 $aRODRIGUES, E. C.
700 1 $aRAMOS, E. M.
773 $tPoultry Science$gv. 93, p. 755-761, 2014.
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