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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos; Embrapa Gado de Leite. |
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Data corrente: |
19/07/2018 |
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Data da última atualização: |
05/04/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SANTOS, A. S. de O. dos; MEURER, V. M.; COSTA, F. F.; PAIVA, I. M. de; FOGAÇA, G. N.; EGITO, A. S. do; FURTADO, M. A. M.; MARTINS, M. F. |
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Afiliação: |
Alessa Siqueira de O. dos Santos; Vaneida Maria Meurer; Fabiano Freire Costa; Igor Moura de Paiva; Gisele Nogueira Fogaça; ANTÔNIO SILVIO DO EGITO, CNPC; Marco Antonio Moreira Furtado; Marta Fonseca Martins. |
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Título: |
Major goat milk protein: Separation and characterization by "lab-on-a-chip" microfluidic electrophoresis. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Boletim do Centro Pesquisa Processamento de Alimentos, Curitiba, v. 35, n. 2, jul./dez. 2017. |
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DOI: |
10.5380/bceppa.v35i2.60308 |
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Idioma: |
Inglês |
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Notas: |
Boletim do CEPPA. |
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Conteúdo: |
This work presents the electrophoretic profile of goat and cow milk samples and their mixtures using microfluidic and conventional electrophoresis. The microfluidic method allowed the separation of the major caseins from milk, excepting the goat kappa-casein. Besides, the major whey proteins were separated with perfect distinction of A and B beta-lactoglobulin variants. Comparing to SDS-PAGE, a variation in the molecular weight was observed in all milk proteins. However, A and B beta-lactoglobulin variants could not be isolated using SDS-PAGE. Although urea-PAGE did not show high resolution among whey proteins, y-, k-, beta-, and alpha-caseins were clearly identified. This method also showed a lower limit detection of cow milk in mixture samples than the "lab-on-a-chip" electrophoresis. In both methods, the highest linearity obtained from plotting total percentage against cow milk concentration was observed by using cow ?s1-casein (R2 = 0.986 and R² = 0.973). This result indicates that microfluidic electrophoresis is an effective tool to detect the presence of some proteins in goat and cow milk, and in mixtures. Microfluidic chip technology might will complement the current methods for analyzing milk proteins, highlighting its speed amount of reagents and whey protein separation, which showed a better result than urea or SDS-PAGE |
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Palavras-Chave: |
Cow milk; Protein quantification. |
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Thesaurus Nal: |
Alpha-casein; Beta-casein; Kappa-casein; Milk proteins; Polyacrylamide; Urea. |
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Categoria do assunto: |
Q Alimentos e Nutrição Humana |
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Marc: |
LEADER 02340naa a2200325 a 4500
001 2093410
005 2019-04-05
008 2017 bl uuuu u00u1 u #d
024 7 $a10.5380/bceppa.v35i2.60308$2DOI
100 1 $aSANTOS, A. S. de O. dos
245 $aMajor goat milk protein$bSeparation and characterization by "lab-on-a-chip" microfluidic electrophoresis.$h[electronic resource]
260 $c2017
500 $aBoletim do CEPPA.
520 $aThis work presents the electrophoretic profile of goat and cow milk samples and their mixtures using microfluidic and conventional electrophoresis. The microfluidic method allowed the separation of the major caseins from milk, excepting the goat kappa-casein. Besides, the major whey proteins were separated with perfect distinction of A and B beta-lactoglobulin variants. Comparing to SDS-PAGE, a variation in the molecular weight was observed in all milk proteins. However, A and B beta-lactoglobulin variants could not be isolated using SDS-PAGE. Although urea-PAGE did not show high resolution among whey proteins, y-, k-, beta-, and alpha-caseins were clearly identified. This method also showed a lower limit detection of cow milk in mixture samples than the "lab-on-a-chip" electrophoresis. In both methods, the highest linearity obtained from plotting total percentage against cow milk concentration was observed by using cow ?s1-casein (R2 = 0.986 and R² = 0.973). This result indicates that microfluidic electrophoresis is an effective tool to detect the presence of some proteins in goat and cow milk, and in mixtures. Microfluidic chip technology might will complement the current methods for analyzing milk proteins, highlighting its speed amount of reagents and whey protein separation, which showed a better result than urea or SDS-PAGE
650 $aAlpha-casein
650 $aBeta-casein
650 $aKappa-casein
650 $aMilk proteins
650 $aPolyacrylamide
650 $aUrea
653 $aCow milk
653 $aProtein quantification
700 1 $aMEURER, V. M.
700 1 $aCOSTA, F. F.
700 1 $aPAIVA, I. M. de
700 1 $aFOGAÇA, G. N.
700 1 $aEGITO, A. S. do
700 1 $aFURTADO, M. A. M.
700 1 $aMARTINS, M. F.
773 $tBoletim do Centro Pesquisa Processamento de Alimentos, Curitiba$gv. 35, n. 2, jul./dez. 2017.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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Registro Completo
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
29/09/2021 |
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Data da última atualização: |
02/10/2021 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
ALVARENGA, R. C.; BORGHI, E.; GONTIJO NETO, M. M.; RESENDE, A. V. de; CALONEGO, J. C.; SILVEIRA, M. C. T. da; KARAM, D.; SIMEÃO, R. M. |
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Afiliação: |
RAMON COSTA ALVARENGA, CNPMS; EMERSON BORGHI, CNPMS; MIGUEL MARQUES GONTIJO NETO, CNPMS; ALVARO VILELA DE RESENDE, CNPMS; JULIANO CARLOS CALONEGO; MARCIA CRISTINA T DA SILVEIRA, CPPSUL; DECIO KARAM, CNPMS; ROSANGELA MARIA SIMEAO, CNPGC. |
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Título: |
Agricultural productivity of a long-term crop-livestock system in the Cerrado Biome, Brazil. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
In: WORLD CONGRESS ON INTEGRATED CROP-LIVESTOCK-FORESTRY SYSTEMS, 2., 2021. Proceedings reference. Brasília, DF: Embrapa, 2021. |
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Páginas: |
p. 487-491. |
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ISBN: |
978-65-994135-4-4 |
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Idioma: |
Inglês |
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Notas: |
WCCLF.
Evento online. |
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Conteúdo: |
Some regions of the Cerrado Biome present edaphoclimatic conditions restrictive to the maximum productive potential of agriculture and livestock. In these regions, experiments with crop-livestock system (CLS) show that this strategy allows the intensification of agricultural production in a sustainable manner. Rotation, intercropping and succession of crops producing grains or silage with forage species, and conservationist soil management such as no-till and correction of the soil profile favorable to root growth, allow the exploitation of the agricultural area, especially when there is a water deficit. In fifteen years of conducting the CLS, the production of agricultural crops met the demand for food and, also, provided grain yields above regional averages, and even national averages. In addition to the lower risk, the strategy adopted met internal demand and generated surplus grain and silage for commercialization, increasing income. In the temporal analysis of the CLS, the adoption of sustainable soil and crop management practices has, over time, overcome the region's edaphoclimatic limitations, in an economically viable way, based on adequate land use planning, and by the choice of appropriate combination of crops, properly aligned with the livestock activity of interest. |
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Palavras-Chave: |
Agricultural production; Consortium. |
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Thesagro: |
Cerrado; Consorciação de Cultura; Plantio Direto; Rotação de Cultura. |
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Thesaurus NAL: |
Crop rotation; No-tillage. |
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Categoria do assunto: |
A Sistemas de Cultivo |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/226601/1/Agricultural-productivity.pdf
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Marc: |
LEADER 02329nam a2200325 a 4500
001 2134877
005 2021-10-02
008 2021 bl uuuu u00u1 u #d
020 $a978-65-994135-4-4
100 1 $aALVARENGA, R. C.
245 $aAgricultural productivity of a long-term crop-livestock system in the Cerrado Biome, Brazil.$h[electronic resource]
260 $aIn: WORLD CONGRESS ON INTEGRATED CROP-LIVESTOCK-FORESTRY SYSTEMS, 2., 2021. Proceedings reference. Brasília, DF: Embrapa$c2021
300 $ap. 487-491.
500 $aWCCLF. Evento online.
520 $aSome regions of the Cerrado Biome present edaphoclimatic conditions restrictive to the maximum productive potential of agriculture and livestock. In these regions, experiments with crop-livestock system (CLS) show that this strategy allows the intensification of agricultural production in a sustainable manner. Rotation, intercropping and succession of crops producing grains or silage with forage species, and conservationist soil management such as no-till and correction of the soil profile favorable to root growth, allow the exploitation of the agricultural area, especially when there is a water deficit. In fifteen years of conducting the CLS, the production of agricultural crops met the demand for food and, also, provided grain yields above regional averages, and even national averages. In addition to the lower risk, the strategy adopted met internal demand and generated surplus grain and silage for commercialization, increasing income. In the temporal analysis of the CLS, the adoption of sustainable soil and crop management practices has, over time, overcome the region's edaphoclimatic limitations, in an economically viable way, based on adequate land use planning, and by the choice of appropriate combination of crops, properly aligned with the livestock activity of interest.
650 $aCrop rotation
650 $aNo-tillage
650 $aCerrado
650 $aConsorciação de Cultura
650 $aPlantio Direto
650 $aRotação de Cultura
653 $aAgricultural production
653 $aConsortium
700 1 $aBORGHI, E.
700 1 $aGONTIJO NETO, M. M.
700 1 $aRESENDE, A. V. de
700 1 $aCALONEGO, J. C.
700 1 $aSILVEIRA, M. C. T. da
700 1 $aKARAM, D.
700 1 $aSIMEÃO, R. M.
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Embrapa Milho e Sorgo (CNPMS) |
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