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Registro Completo |
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Biblioteca(s): |
Embrapa Pantanal. |
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Data corrente: |
27/06/1996 |
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Data da última atualização: |
04/04/2017 |
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Autoria: |
EAGLESOME, M. D.; SAMPATH, M. I.; GARCIA, M. M. |
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Título: |
A detection assay for Campylobcter fetus in bovine semen by restriction analysis of PCR amplified DNA. |
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Ano de publicação: |
1995 |
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Fonte/Imprenta: |
Veterinary Research Communications, v.19, n.4, p.253-263, 1995. |
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Idioma: |
Inglês |
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Conteúdo: |
A rapid screening assay for Campylobacter fetus in bull semen was developed using the polymerase chain reaction (PCR) and restriction endonuclease analysis (REA) to complement isolation by culture. An oligonucleotide primer par (C1/C2) from the hypervariable region of 16S rRNA of C. fetus was used to amplify a 362 base pair fragment by PCR. The PCR/REA assay, which is completed in 10 hours, detected as few as three C. fetus subsp. venerealis cells in experimentally infected raw bull smenen and in semen diluted with milk or egg yolk Tris (EYT). All the strains tested, of both subspecies of C. fetus, were amplified, as were some other Campylobacter species. Restrictring the amplified products by AluI differentiated C. fetus from the other organisms. There was no visible product generated by PCR from S. sputorum subsp. bubulus, a saprophytic organim found in the prepuce of bulls, or from seven other species of bacteria found in semen. A modification of the PCR assay, using another primer pair (C3/C2) and two temeprature PCR cycling conditions, increased the probability of detecting C. fetus sunsp. venereallis. PCR amplification followed by REA could be used to screen bovine semen rapidly for C. fetus. In most cases, sequencing of C1/C2 PCR generated products would be preferable for distinguishing between the two subspecies of C. fetus. |
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Palavras-Chave: |
Bovine; Bull; PCR. |
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Thesagro: |
Bovino; Campylobacter Fetus; Sêmen; Touro. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01976naa a2200229 a 4500
001 1789228
005 2017-04-04
008 1995 bl --- 0-- u #d
100 1 $aEAGLESOME, M. D.
245 $aA detection assay for Campylobcter fetus in bovine semen by restriction analysis of PCR amplified DNA.
260 $c1995
520 $aA rapid screening assay for Campylobacter fetus in bull semen was developed using the polymerase chain reaction (PCR) and restriction endonuclease analysis (REA) to complement isolation by culture. An oligonucleotide primer par (C1/C2) from the hypervariable region of 16S rRNA of C. fetus was used to amplify a 362 base pair fragment by PCR. The PCR/REA assay, which is completed in 10 hours, detected as few as three C. fetus subsp. venerealis cells in experimentally infected raw bull smenen and in semen diluted with milk or egg yolk Tris (EYT). All the strains tested, of both subspecies of C. fetus, were amplified, as were some other Campylobacter species. Restrictring the amplified products by AluI differentiated C. fetus from the other organisms. There was no visible product generated by PCR from S. sputorum subsp. bubulus, a saprophytic organim found in the prepuce of bulls, or from seven other species of bacteria found in semen. A modification of the PCR assay, using another primer pair (C3/C2) and two temeprature PCR cycling conditions, increased the probability of detecting C. fetus sunsp. venereallis. PCR amplification followed by REA could be used to screen bovine semen rapidly for C. fetus. In most cases, sequencing of C1/C2 PCR generated products would be preferable for distinguishing between the two subspecies of C. fetus.
650 $aBovino
650 $aCampylobacter Fetus
650 $aSêmen
650 $aTouro
653 $aBovine
653 $aBull
653 $aPCR
700 1 $aSAMPATH, M. I.
700 1 $aGARCIA, M. M.
773 $tVeterinary Research Communications$gv.19, n.4, p.253-263, 1995.
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Registro original: |
Embrapa Pantanal (CPAP) |
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Registro Completo
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Biblioteca(s): |
Embrapa Acre; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
12/09/2016 |
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Data da última atualização: |
02/07/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
AZÊVEDO, H. S. F. S.; SOUSA, A. C. B.; MARTINS, K.; OLIVEIRA, J. C.; TEIXEIRA, R. B.; SILVA, L. M. da; VALLS, J. F. M.; ASSIS, G. M. L. de; CAMPOS, T. de. |
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Afiliação: |
Programa de Pós-Graduação em Biotecnologia e Recursos Genéticos da Rede Bionorte, Embrapa Rondônia; Universidade Federal da Paraíba; Universidade Federal de São Carlos; Universidade Federal do Acre; RENATA BELTRAO TEIXEIRA YOMURA, CPAF-Acre; LUCIELIO MANOEL DA SILVA, CPAF-Acre; JOSE FRANCISCO MONTENEGRO VALLS, Cenargen; GISELLE MARIANO LESSA DE ASSIS, CPAF-Acre; TATIANA DE CAMPOS, CPAF-Acre. |
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Título: |
Genetic diversity of the forage peanut in the Jequitinhonha, São Francisco, and Paranã River valleys of Brazil. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Genetics and Molecular Research, Ribeirão Preto, v. 15, n. 3, Sept. 2016. |
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ISSN: |
1676-5680 |
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DOI: |
10.4238/gmr.15038601 |
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Idioma: |
Inglês |
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Conteúdo: |
Arachis pintoi and A. repens are legumes with a high forage value that are used to feed ruminants in consortium systems. Not only do they increase the persistence and quality of pastures, they are also used for ornamental and green cover. The objective of this study was to analyze microsatellite markers in order to access the genetic diversity of 65 forage peanut germplasm accessions in the section Caulorrhizae of the genus Arachis in the Jequitinhonha, São Francisco and Paranã River valleys of Brazil. Fifty-seven accessions of A. pintoi and eight of A. repens were analyzed using 17 microsatellites, and the observed heterozygosity (HO), expected heterozygosity (HE), number of alleles per locus, discriminatory power, and polymorphism information content were all estimated. Ten loci (58.8%) were polymorphic, and 125 alleles were found in total. The HE ranged from 0.30 to 0.94, and HO values ranged from 0.03 to 0.88. By using Bayesian analysis, the accessions were genetically differentiated into three gene pools. Neither the unweighted pair group method with arithmetic mean nor a neighbor-joining analysis clustered samples into species, origin, or collection area. These results reveal a very weak genetic structure that does not form defined clusters, and that there is a high degree of similarity between the two species. |
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Palavras-Chave: |
Acesso; Amendoim forrageiro; Arachis repens; Diversidade genética; Forage peanut; Leguminosas forrajeras; Marcador microssatélite; Marcadores genéticos; Marcadores microssatélites; Pastizales; Repeticiones de microsatélite; Vale do Jequitinhonha; Vale do Rio Paraná; Vale do Rio São Francisco; Variación genética. |
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Thesagro: |
Leguminosa Forrageira; Marcador genético; Pastagem; Variação genética. |
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Thesaurus NAL: |
Arachis pintoi; Forage legumes; Genetic markers; Genetic variation; Microsatellite repeats; Pastures. |
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Categoria do assunto: |
K Ciência Florestal e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/147293/1/26116.pdf
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Marc: |
LEADER 02978naa a2200541 a 4500
001 2052708
005 2021-07-02
008 2016 bl uuuu u00u1 u #d
022 $a1676-5680
024 7 $a10.4238/gmr.15038601$2DOI
100 1 $aAZÊVEDO, H. S. F. S.
245 $aGenetic diversity of the forage peanut in the Jequitinhonha, São Francisco, and Paranã River valleys of Brazil.$h[electronic resource]
260 $c2016
520 $aArachis pintoi and A. repens are legumes with a high forage value that are used to feed ruminants in consortium systems. Not only do they increase the persistence and quality of pastures, they are also used for ornamental and green cover. The objective of this study was to analyze microsatellite markers in order to access the genetic diversity of 65 forage peanut germplasm accessions in the section Caulorrhizae of the genus Arachis in the Jequitinhonha, São Francisco and Paranã River valleys of Brazil. Fifty-seven accessions of A. pintoi and eight of A. repens were analyzed using 17 microsatellites, and the observed heterozygosity (HO), expected heterozygosity (HE), number of alleles per locus, discriminatory power, and polymorphism information content were all estimated. Ten loci (58.8%) were polymorphic, and 125 alleles were found in total. The HE ranged from 0.30 to 0.94, and HO values ranged from 0.03 to 0.88. By using Bayesian analysis, the accessions were genetically differentiated into three gene pools. Neither the unweighted pair group method with arithmetic mean nor a neighbor-joining analysis clustered samples into species, origin, or collection area. These results reveal a very weak genetic structure that does not form defined clusters, and that there is a high degree of similarity between the two species.
650 $aArachis pintoi
650 $aForage legumes
650 $aGenetic markers
650 $aGenetic variation
650 $aMicrosatellite repeats
650 $aPastures
650 $aLeguminosa Forrageira
650 $aMarcador genético
650 $aPastagem
650 $aVariação genética
653 $aAcesso
653 $aAmendoim forrageiro
653 $aArachis repens
653 $aDiversidade genética
653 $aForage peanut
653 $aLeguminosas forrajeras
653 $aMarcador microssatélite
653 $aMarcadores genéticos
653 $aMarcadores microssatélites
653 $aPastizales
653 $aRepeticiones de microsatélite
653 $aVale do Jequitinhonha
653 $aVale do Rio Paraná
653 $aVale do Rio São Francisco
653 $aVariación genética
700 1 $aSOUSA, A. C. B.
700 1 $aMARTINS, K.
700 1 $aOLIVEIRA, J. C.
700 1 $aTEIXEIRA, R. B.
700 1 $aSILVA, L. M. da
700 1 $aVALLS, J. F. M.
700 1 $aASSIS, G. M. L. de
700 1 $aCAMPOS, T. de
773 $tGenetics and Molecular Research, Ribeirão Preto$gv. 15, n. 3, Sept. 2016.
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Embrapa Acre (CPAF-AC) |
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