Data/hora: 10/06/2024 12:01:20

Biblioteca(s): | Embrapa Pantanal.
Data corrente: | 27/06/1996
Data da última atualização: | 04/04/2017
Autoria: | EAGLESOME, M. D.; SAMPATH, M. I.; GARCIA, M. M.
Título: | A detection assay for Campylobcter fetus in bovine semen by restriction analysis of PCR amplified DNA.
Ano de publicação: | 1995
Fonte/Imprenta: | Veterinary Research Communications, v.19, n.4, p.253-263, 1995.
Idioma: | Inglês
Conteúdo: | A rapid screening assay for Campylobacter fetus in bull semen was developed using the polymerase chain reaction (PCR) and restriction endonuclease analysis (REA) to complement isolation by culture. An oligonucleotide primer par (C1/C2) from the hypervariable region of 16S rRNA of C. fetus was used to amplify a 362 base pair fragment by PCR. The PCR/REA assay, which is completed in 10 hours, detected as few as three C. fetus subsp. venerealis cells in experimentally infected raw bull smenen and in semen diluted with milk or egg yolk Tris (EYT). All the strains tested, of both subspecies of C. fetus, were amplified, as were some other Campylobacter species. Restrictring the amplified products by AluI differentiated C. fetus from the other organisms. There was no visible product generated by PCR from S. sputorum subsp. bubulus, a saprophytic organim found in the prepuce of bulls, or from seven other species of bacteria found in semen. A modification of the  PCR assay, using another primer pair (C3/C2) and two temeprature PCR cycling conditions, increased the probability of detecting C. fetus sunsp. venereallis. PCR amplification followed by REA could be used to screen bovine semen rapidly for C. fetus. In most cases, sequencing of C1/C2 PCR generated products would be preferable for distinguishing between the two subspecies of C. fetus.
Palavras-Chave: | Bovine; Bull; PCR.
Thesagro: | Bovino; Campylobacter Fetus; Sêmen; Touro.
Categoria do assunto: | --
Registro original: | Embrapa Pantanal (CPAP)
Exemplares: | CPAP - (32583-1) - ADD - SP - PP - SP14891 -  - 14891 - DIS