Data/hora: 10/06/2024 12:55:33

Biblioteca(s): | Embrapa Pantanal.
Data corrente: | 19/04/1996
Data da última atualização: | 04/04/2017
Autoria: | BIELANSKI, A.; SAMPATH, M.; GRADIL, C.; EAGLESOME, M. D.; GARCIA, M.
Título: | In vitro fertilization of bovine ova in the presence of Campylobacter fetus subsp. venerealis.
Ano de publicação: | 1994
Fonte/Imprenta: | Reproduction Domestic Animals, v.29, n.8, p.488-493, 1994.
Idioma: | Inglês
Conteúdo: | The objective of this study were to examine the effect of Campylobacter fetus subsp. venerealis (Cfv) on the in vitro fertilization (IVF) of bovine ova and to detect Cfv associated with IVF produced embryos. Cumulus-oocyte complexes were matured for 24 h in TCM-199 and were fertilized with swim-up spermatozoa prepared in modified Tyrodes medium supplemented with BSA (6 mg/ml) and heparin (15 ug/ml) with or without 1 x 10 Cfv organisms/ml. Thereafter, zygotes were cultured for 8 days in TCM-199 medium containing oviductal epithelial cells. In comparing ova inseminated with spermatozoa either treated or untreated with Cfv, no significant differences were observed in the percentages of: 1. Ova fertilized (72% vs. 76%); 2. Fertilized ova which elevated (52% vs. 60%); or 3. Embryos that developed to the blastocyst stage (31% vs.41%). Motile Cfv were observed microscopically in the fertilization medium 48 h after insemination. A high proportion of cumulus-free uncleaved and cleaved oocytes washed 10 times 24 h after insemintion tested positive for the presence of Cfv. After 7 days of in vitro culture, however, embryos were free from the organisms as determined by culture. However, in some embryos the presence of Cfv DNA was detected using polymerase-chain-reaction technology.
Palavras-Chave: | Bivine; Doenca venerea; Venereal disease.
Thesagro: | Bovino; Campylobacter Fetus.
Categoria do assunto: | --
Registro original: | Embrapa Pantanal (CPAP)
Exemplares: | CPAP - (31613-1) - ADC - SP - PP - SP14806 -  - 14806 - DIS