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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
29/08/1995 |
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Data da última atualização: |
17/05/2023 |
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Autoria: |
MARIN, M. L.; DURAN-VILA, N. |
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Afiliação: |
Instituto Valenciano de Investigaciones Agrarias (IVIA), Apartado oficial, 46113 Moncada (Valencia), Spain; (requests for offprints). |
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Título: |
Survival of somatic embryos and recovery of plants of sweet orange (Citrus sinensis (L.) Obs.) after immersion in liquid nitrogen. |
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Ano de publicação: |
1988 |
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Fonte/Imprenta: |
Plant Cell, Tissue and Organ Culture, v.14, p.51-57, 1988. |
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Idioma: |
Inglês |
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Conteúdo: |
Somatic embryos of `Washington Navel' sweet orange (Citrus sinensis (L.) Obs.)derived from in vitro cultured ovules excised from immature fruits, were frozen to the temperature of liquid nitrogen. A method of slow cooling at a rate of 0.5grausC min-1 down to-42grausc followed by storage in liquid nitrogen was used. Thawing was achieved by keeping the specimens at room temperature for 15 min. A small number of frozen embryos survived and developed into proliferating cultures that produced whole plants. The plants. obtained from frozen cultures were transferred to soil and are growing successfully. |
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Palavras-Chave: |
Cryoprotection; Liquid nitrogen; Pseudobuldi. |
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Thesagro: |
Citrus Sinensis. |
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Thesaurus Nal: |
cryopreservation; somatic embryos. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01256naa a2200205 a 4500
001 1646904
005 2023-05-17
008 1988 bl uuuu u00u1 u #d
100 1 $aMARIN, M. L.
245 $aSurvival of somatic embryos and recovery of plants of sweet orange (Citrus sinensis (L.) Obs.) after immersion in liquid nitrogen.$h[electronic resource]
260 $c1988
520 $aSomatic embryos of `Washington Navel' sweet orange (Citrus sinensis (L.) Obs.)derived from in vitro cultured ovules excised from immature fruits, were frozen to the temperature of liquid nitrogen. A method of slow cooling at a rate of 0.5grausC min-1 down to-42grausc followed by storage in liquid nitrogen was used. Thawing was achieved by keeping the specimens at room temperature for 15 min. A small number of frozen embryos survived and developed into proliferating cultures that produced whole plants. The plants. obtained from frozen cultures were transferred to soil and are growing successfully.
650 $acryopreservation
650 $asomatic embryos
650 $aCitrus Sinensis
653 $aCryoprotection
653 $aLiquid nitrogen
653 $aPseudobuldi
700 1 $aDURAN-VILA, N.
773 $tPlant Cell, Tissue and Organ Culture$gv.14, p.51-57, 1988.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
