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Registro Completo |
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
03/07/1995 |
Data da última atualização: |
03/07/1995 |
Autoria: |
SEMANCIK, J. S.; ROISTACHER, C. N., RIVERA-BUSTAMANTE, R.; DURAN-VILA,N. |
Afiliação: |
Departamento of Plant Pathology and Cell Interaction Research Group, University of California, Riverside, California 92521, U.S.A. |
Título: |
Citrus cachexia viroid, a new viroid of citrus: Relationship to viroids of the exocortis disease complex. |
Ano de publicação: |
1988 |
Fonte/Imprenta: |
Journal of genetique virology, v.69, p.3059-3068, 1988. |
Idioma: |
Inglês |
Conteúdo: |
Recovery of highly purified citrus cachexia viroid (CCaV) was accomplished by serial elution followung CF-ll cellulose chromatography of a 2 m-LiCl-soluble nucleic acid preparation. The alternative herbaceous host, cucumber (Cucumis sativus cv. Suyo), yielded greater quantities of the viroid than the highest yielding citrus host, citron (citrus medica cv. Etrog). A randomly primed cDNA probe to CCaV purified from cucumber reacted positively to extracts from citron and cucumber inoculated with the same isolate of CCaV. When tested against a broad range of other citrus viroids, the CCaV cDNA hybridized to only one, CVIIa, which has been identified as the causalagent of a mild form of the citrus exocortis disease. Because of the apparent homology between the nuclotide sequences of CV-IIa and CCaV, and a size difference of only five to ten nucleotides, these RNAs can be considered as members of a common subgroup of citrus viroids. These two viroids have been classified by bioassay reactions as the causal agents of two distinct types of citrus disease, an exocortis-like syndrome and cachexia. The properties of and relationships between these two members of the citrus viroid II group and the definition of the exocortis and cachexia (xyloporosis)diseases are presented. |
Thesagro: |
Cidra; Citrus Medica; Eletroforese; Laranja Doce; RNA. |
Categoria do assunto: |
-- |
Marc: |
LEADER 01885naa a2200205 a 4500 001 1646621 005 1995-07-03 008 1988 bl --- 0-- u #d 100 1 $aSEMANCIK, J. S. 245 $aCitrus cachexia viroid, a new viroid of citrus$bRelationship to viroids of the exocortis disease complex. 260 $c1988 520 $aRecovery of highly purified citrus cachexia viroid (CCaV) was accomplished by serial elution followung CF-ll cellulose chromatography of a 2 m-LiCl-soluble nucleic acid preparation. The alternative herbaceous host, cucumber (Cucumis sativus cv. Suyo), yielded greater quantities of the viroid than the highest yielding citrus host, citron (citrus medica cv. Etrog). A randomly primed cDNA probe to CCaV purified from cucumber reacted positively to extracts from citron and cucumber inoculated with the same isolate of CCaV. When tested against a broad range of other citrus viroids, the CCaV cDNA hybridized to only one, CVIIa, which has been identified as the causalagent of a mild form of the citrus exocortis disease. Because of the apparent homology between the nuclotide sequences of CV-IIa and CCaV, and a size difference of only five to ten nucleotides, these RNAs can be considered as members of a common subgroup of citrus viroids. These two viroids have been classified by bioassay reactions as the causal agents of two distinct types of citrus disease, an exocortis-like syndrome and cachexia. The properties of and relationships between these two members of the citrus viroid II group and the definition of the exocortis and cachexia (xyloporosis)diseases are presented. 650 $aCidra 650 $aCitrus Medica 650 $aEletroforese 650 $aLaranja Doce 650 $aRNA 700 1 $aROISTACHER, C. N., RIVERA-BUSTAMANTE, R. 700 1 $aDURAN-VILA,N. 773 $tJournal of genetique virology$gv.69, p.3059-3068, 1988.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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Registros recuperados : 6 | |
5. | | MURCIA, N.; BEDERSKI, K.; WULFF, N. A.; BARBOSA, C. de J.; BOVÉ, J. M.; DURAN-VILA, N. Viroids in Tahiti limes showing bark cracking symptoms. In: CONFERENCE OF THE INTERNATIONAL ORGANIZATION OF CITRUS VIROLOGISTS, 17., 2007, Adana. Program & Abstracts. Adana: [s.n.], 2007. p.103.Tipo: Resumo em Anais de Congresso | Circulação/Nível: -- - -- |
Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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6. | | MURCIA, N.; BANI HASHEMIAN, S. M.; BEDERSKI, K.; WULFF, N. A.; BARBOSA, C. de J.; BOVÉ, J. M.; DURAN-VILA, N. Viroids in Tahiti lime scions showing back cracking symptoms. In: CONFERENCE OF THE INTERNATIONAL ORGANIZATION OF CITRUS VIROLOGISTS, 17., 2007, Adana. Proceedings... [S.l.]: IOCV, 2010. Publications. Disponível em: Acesso em: 11 mar. 2011.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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Registros recuperados : 6 | |
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