Data/hora: 03/06/2024 17:51:12

Biblioteca(s): | Embrapa Mandioca e Fruticultura.
Data corrente: | 01/12/1993
Data da última atualização: | 01/12/1993
Autoria: | DURAN-VILA, N.; FLORES, S. R.; SEMANIK, J. S.
Afiliação: | Instituto Valenciano de Investigaciones Agrarias (IVIA), Moncada, Valencia, Spain; Instituto de Agroquimica y Tecnologia de Alimentos (CSIC) Valencia, Spain.
Título: | Characterization of viroid-like RNAs associated with the citrus exocortis syndrome.
Ano de publicação: | 1986
Fonte/Imprenta: | Virology, v.150, n.1, p.75-84, 1986.
Idioma: | Inglês
Conteúdo: | The additional viroid-like RNA species (RNA-I, -II, and -III), smaller than the 371-nucleotide citrus exocortis viroid (CEV), have been identified in citron (Citrus medica) trees which display aymptoms of the exocortis disease. The three RNAs migrate on polyacrylamide gels under denaturing conditions in the region between CEV and avocado sunblotch viroid molecules, indicating a size range of about 311-335 nucleotide residues in both circular and linear molecular forms. RNA-II is removed from the preparations when chromatographed on  CF-11 cellulose. All three RNAs fail to replicate when introduced into Gynura aurantiaca, a herbaceous host of CEV, yet RNA-I and RNA-III can be independently transmitted to citron. Dot-blot hybridization with CEV-cDNA indicates nosignificant homology between CEV and RNA-I, RNA-II, or RNA-III. The relationship among CEV-RNA and the theree viroid-like RNA species in the segregation of expression and intensity of the exocortis disease reaction is discused.
Palavras-Chave: | Eletioforose; Gynura aurantica; Viroide da exocorte.
Thesagro: | Doença.
Categoria do assunto: | --
Registro original: | Embrapa Mandioca e Fruticultura (CNPMF)
Exemplares: | CNPMF - (15408-1) - ADC - SP - -- - S2076 -  - S2076 - DIS