Data/hora: 10/06/2024 08:05:00

Biblioteca(s): 	Embrapa Mandioca e Fruticultura.	
Data corrente: 	21/09/1993	
Data da última atualização: 	21/09/1993	
Autoria: 	DURAN-VILA, N.; PINA, J. A.; MOLINS, M. I.; NAVARRO, L.	
Afiliação: 	Instituto Valenciano de Investigaciones Agrarias, Apartado Oficial, 46113 Moncada, Valencia, Spain.	
Título: 	A new indexing method for cachexia.	
Ano de publicação: 	1991	
Fonte/Imprenta: 	Riverside, California: International Organization of Citrus Virologists, 1991.	
Páginas: 	p.224-229	
Idioma: 	Inglês	
Notas: 	Conference of the International Organization of Citrus Virologists, 11., 1991, Riverside California, Proceedings...	
Conteúdo: 	The utilization of nucleic acid extraction and polyacrylamide gel electrophoresis as an alternative to the Parson's Special bioassay for routine indexing of cachexia was evaluated. Three field sources of citrus viroids and three greenhouse controls were graft inoculated on Arizona Etrog citron. Inocualated citrons were periodically analyzed by nucleic acid extraction and sequential gel elctrophoresis. The citrus cachexia viroid (CCaV) was detected after 3 months in all the plants tested regardless of the source. Three electrophoresis systems were also compared. The proposed method combines the desirable characteristics of citron as an excellent host for viroid replication and the sensitivity of electrophoresis and silver staining for viroid detection. The method is now routinely used in the Citrus Sanitation Program in Spain.	
Palavras-Chave: 	Citrus cachexia viroid; Citrus viroids; Xyloporosis.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Mandioca e Fruticultura (CNPMF)	
Exemplares: 	CNPMF - (13301-1) - ADC - PL - -- - 634.3040601 - C748p - 1992000183 - DIS