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Registro Completo |
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Biblioteca(s): |
Embrapa Pantanal. |
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Data corrente: |
13/01/2012 |
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Data da última atualização: |
19/02/2013 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ARAUJO, J. P.; NOGUEIRA, M. F.; DUARTE, J. M. B. |
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Afiliação: |
JOAO PESSOA ARAUJO, UNIVERSIDADE ESTADUAL PAULISTA (UNESP); MARCIA FURLAN NOGUEIRA T DE LIMA, CPAP; JOSE M. B. DUARTE, UNIVERSIDADE ESTADUAL PAULISTA. |
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Título: |
Survey for foot-and-mouth disease in the endangered Marsh Deer (Blastocerus dichotomus) from marshlands of the Parana River Basin, Brazil. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Journal of Wildlife Diseases, v. 46, n. 3, p. 939 -943, 2010. |
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Idioma: |
Português |
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Conteúdo: |
Habitat fragmentation and diseases have resulted in a decline of the marsh deer (Blastocerus dichotomus) throughout its South American range. Our objectives were to determine whether marsh deer intended for translocation from a region of the Rio Parana Basin had been infected previously by foot-andmouth disease virus (FMDV) and whether they were carrying virus. We captured marsh deer from June to October 1998 and collected blood from 108 animals and esophageal-pharyngeal fluid from 53. Serum was tested for antibodies against three FMDV serotypes (O, A, and C) by liquid-phase-blocking sandwich enzyme-linked immunosorbent assay (ELISA). Esophagealpharyngeal fluid was tested for FMDV RNA by reverse transcription polymerase chain reaction (RT-PCR) and inoculation into three successive baby hamster kidney (BHK-21) cell subcultures, followed by RT-PCR of cultures. We detected low log10 titers (range 1.0?1.5) to FMDV subtype A24 Cruzeiro in 19 of 108 sampled marsh deer, but failed to isolate FMDV or detect FMDV RNA in any samples. We conclude that marsh deer from our study site were unlikely to carry FMDV; however, as a preventive measure, the 19 animals with titers for FMDV were not sent to FMDV-free Brazilian states. |
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Palavras-Chave: |
Serologic survey. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 01752naa a2200157 a 4500
001 1912569
005 2013-02-19
008 2010 bl --- 0-- u #d
100 1 $aARAUJO, J. P.
245 $aSurvey for foot-and-mouth disease in the endangered Marsh Deer (Blastocerus dichotomus) from marshlands of the Parana River Basin, Brazil.
260 $c2010
520 $aHabitat fragmentation and diseases have resulted in a decline of the marsh deer (Blastocerus dichotomus) throughout its South American range. Our objectives were to determine whether marsh deer intended for translocation from a region of the Rio Parana Basin had been infected previously by foot-andmouth disease virus (FMDV) and whether they were carrying virus. We captured marsh deer from June to October 1998 and collected blood from 108 animals and esophageal-pharyngeal fluid from 53. Serum was tested for antibodies against three FMDV serotypes (O, A, and C) by liquid-phase-blocking sandwich enzyme-linked immunosorbent assay (ELISA). Esophagealpharyngeal fluid was tested for FMDV RNA by reverse transcription polymerase chain reaction (RT-PCR) and inoculation into three successive baby hamster kidney (BHK-21) cell subcultures, followed by RT-PCR of cultures. We detected low log10 titers (range 1.0?1.5) to FMDV subtype A24 Cruzeiro in 19 of 108 sampled marsh deer, but failed to isolate FMDV or detect FMDV RNA in any samples. We conclude that marsh deer from our study site were unlikely to carry FMDV; however, as a preventive measure, the 19 animals with titers for FMDV were not sent to FMDV-free Brazilian states.
653 $aSerologic survey
700 1 $aNOGUEIRA, M. F.
700 1 $aDUARTE, J. M. B.
773 $tJournal of Wildlife Diseases$gv. 46, n. 3, p. 939 -943, 2010.
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Embrapa Pantanal (CPAP) |
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| 5. |  | ASSIS, O. B. G. Water sorption in chitosan nanoporous films. In: REUNIÃO DA REDE DE NANOBIOTECNOLOGIA - Rede Nanobiotec, 2., 2003, Campinas. Livro de Resumos... Campinas: UNICAMP, 2003. v. 2. p. Seminário de Encerramento das Atividades da Primeira Fase da Rede de Nanobiotecnologia. p. 33-34.| Biblioteca(s): Embrapa Instrumentação. |
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