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Registro Completo |
Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
07/12/2012 |
Data da última atualização: |
13/07/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
DROVAL, A. A.; BINNECK, E.; MARIN, S. R. R.; PAIÃO, F. G.; OBA, A.; NEPOMUCENO, A. L.; SHIMOKOMAKI, M. |
Afiliação: |
ADRIANA A. DROVAL, UEL; ELISEU BINNECK, CNPSO; SILVANA REGINA ROCKENBACH MARIN, CNPSO; FERNANDA G. PAIÃO, UEL; A. OBA, UEL; ALEXANDRE LIMA NEPOMUCENO, SRI; MASSAMI SHIMOKOMAKI, UTFPR. |
Título: |
A new single nucleotide polymorphism in the ryanodine gene of chicken skeletal muscle. |
Ano de publicação: |
2012 |
Fonte/Imprenta: |
Genetics and Molecular Research, Ribeirão Preto, v. 11, n. 2, p. 821-829, 2012. |
Idioma: |
Inglês |
Conteúdo: |
Some genes affect meat quality in chickens. We looked for polymorphisms in the Gallus gallus α-RyR gene (homologous to RyR 1) that could be associated with PSE (pale, soft and exudative) meat. Because RyR genes are over 100,000 bp long and code for proteins with about 5000 amino acids, primers were designed to amplify a fragment of hotspot region 2, a region with a high density of mutations in other species. Total blood DNA was extracted from 50 birds, 25 that had PSE meat and 25 normal chickens. The DNA samples were amplified by PCR, cloned, sequenced, and used to identify single nucleotide polymorphisms (SNPs). The amplified fragment of α-RyR was 604 nucleotides in length; 181 nucleotides were similar to two exons from a hypothetical turkey cDNA sequence for α-RyR. A non-synonymous nucleotide substitution (G/A) was identified in at least one of the three sequenced clones obtained from nine animals, six PSE (HAL+) birds and three normal (HAL-) birds; they were heterozygous for this mutation. |
Thesagro: |
Carne; Genética; Mutação; Qualidade. |
Thesaurus Nal: |
Genetics; Meat quality; Mutation. |
Categoria do assunto: |
S Ciências Biológicas |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/71833/1/new.single.gmr.11.2.2012.pdf
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Marc: |
LEADER 01771naa a2200277 a 4500 001 1941757 005 2017-07-13 008 2012 bl uuuu u00u1 u #d 100 1 $aDROVAL, A. A. 245 $aA new single nucleotide polymorphism in the ryanodine gene of chicken skeletal muscle. 260 $c2012 520 $aSome genes affect meat quality in chickens. We looked for polymorphisms in the Gallus gallus α-RyR gene (homologous to RyR 1) that could be associated with PSE (pale, soft and exudative) meat. Because RyR genes are over 100,000 bp long and code for proteins with about 5000 amino acids, primers were designed to amplify a fragment of hotspot region 2, a region with a high density of mutations in other species. Total blood DNA was extracted from 50 birds, 25 that had PSE meat and 25 normal chickens. The DNA samples were amplified by PCR, cloned, sequenced, and used to identify single nucleotide polymorphisms (SNPs). The amplified fragment of α-RyR was 604 nucleotides in length; 181 nucleotides were similar to two exons from a hypothetical turkey cDNA sequence for α-RyR. A non-synonymous nucleotide substitution (G/A) was identified in at least one of the three sequenced clones obtained from nine animals, six PSE (HAL+) birds and three normal (HAL-) birds; they were heterozygous for this mutation. 650 $aGenetics 650 $aMeat quality 650 $aMutation 650 $aCarne 650 $aGenética 650 $aMutação 650 $aQualidade 700 1 $aBINNECK, E. 700 1 $aMARIN, S. R. R. 700 1 $aPAIÃO, F. G. 700 1 $aOBA, A. 700 1 $aNEPOMUCENO, A. L. 700 1 $aSHIMOKOMAKI, M. 773 $tGenetics and Molecular Research, Ribeirão Preto$gv. 11, n. 2, p. 821-829, 2012.
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Embrapa Soja (CNPSO) |
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Registros recuperados : 4 | |
2. | | DROVAL, A. A.; PRUDENCIO, S. H.; BENASSI, V. T.; ROSSA, A.; PAIAO, F. G.; SHIMOKOMAKI, M. Brazilian citizen and consumer attitudes an preferences regarding broiler breast PSE meat. In: INTERNATIONAL CONGRESS OF MEAT SCIENCE AND TECHNOLOGY, 5Y., 2011, Ghent. Abstracts... Ghent: Belgian Association of Meat Science, 2011. p. 78.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Soja. |
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3. | | DROVAL, A. A.; BENASSI, V. T.; ROSSA, A.; PRUDENCIO, S. H.; PAIÃO, F. G.; SHIMOKOMAKI, M. Consumer attitudes and preferences regarding pale, soft, and exudative broiler breast meat. The Journal of Applied Poultry Researche , Athens, v. 21, n. 3, p. 502-507, Sept. 2012.Tipo: Artigo em Periódico Indexado |
Biblioteca(s): Embrapa Soja. |
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4. | | DROVAL, A. A.; BINNECK, E.; MARIN, S. R. R.; PAIÃO, F. G.; OBA, A.; NEPOMUCENO, A. L.; SHIMOKOMAKI, M. A new single nucleotide polymorphism in the ryanodine gene of chicken skeletal muscle. Genetics and Molecular Research, Ribeirão Preto, v. 11, n. 2, p. 821-829, 2012.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Soja. |
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Registros recuperados : 4 | |
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