Data/hora: 19/06/2024 13:36:58

Biblioteca(s): | Embrapa Clima Temperado.
Data corrente: | 11/03/2021
Data da última atualização: | 12/03/2021
Autoria: | VARGAS, D. P.; CORADIN, J. H.; FERREIRA, L. V.; DORNELES, A. O. S.; VALGAS, R. A.; PEREIRA, A. da S.; CASTRO, C. M.; DUTRA, L. F.
Afiliação: | DAIANE PEIXOTO VARGAS; JULIANA HEY CORADIN, CPACT; LETÍCIA VANNI FERREIRA; ATHOS ODIN SEVERO DORNELES, UFPEL; RICARDO ALEXANDRE VALGAS, CPACT; ARIONE DA SILVA PEREIRA, CPACT; CAROLINE MARQUES CASTRO, CPACT; LEONARDO FERREIRA DUTRA, CPACT.
Título: | Osmoregulators on in vitro encapsulation of potato "BRS Clara" buds.
Ano de publicação: | 2020
Fonte/Imprenta: | Plant Cell Culture and Micropropagation, v. 16, e155, 2020.
Páginas: | 6 p.
ISSN: | 1808-9909
Idioma: | Inglês
Conteúdo: | Synthetic or artificial seeds via encapsulated propagules could be used for in vitro regeneration and mass multiplication, for germplasm preservation and exchange of plant materials. An in vitro encapsulation of potato ?BRS Clara? buds efficient protocol was established. Lateral buds were immersed in a 4% sodium alginate matrix, dripped in CaCl2 for 20 minutes in different osmoregulatory agents: sucrose (8.72, 15.00, 21.28 and 30.00 g L-1) and mannitol (0.00, 11.63, 20.0, 28.37 and 40.0 g L-1). They were then held for 30 days at approximately 4°C and subsequently immersed in KNO3 for capsule decomplexing. Afterward, they were inoculated in MS medium with 3% sucrose and cultured in a growth room under irradiance of 43 &#956;mol m-2 s-1, 16 hours-photoperiod and 25±2 °C temperature. Sucrose and mannitol at concentrations of 15 g L-1 and 20 g L-1, respectively, associated with the encapsulation matrix promoted 80% survival rate of ?BRS Clara? encapsulated buds.
Palavras-Chave: | BRS Clara.
Thesagro: | Batata.
Categoria do assunto: | --
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/221827/1/Artigo-Plant-Cell-Cultura-Micropropagation.pdf
Registro original: | Embrapa Clima Temperado (CPACT)
Exemplares: | CPACT - (21877-1) - UPC - AP - DD -  -  -  - DIS