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Biblioteca(s): |
Embrapa Agrobiologia. |
Data corrente: |
05/01/2001 |
Data da última atualização: |
05/01/2001 |
Autoria: |
RITCHIE, N. J.; SCHUTTER, M. E.; DICK, R. P.; MYROLD, D. D. |
Título: |
Use of length heterogeneity PCR and fatty acid methyl ester profiles to characterize microbial communities in soil. |
Ano de publicação: |
2000 |
Fonte/Imprenta: |
Applied and Environmental Microbiology, Washington, v. 66, p. 1668-1675, 2000. |
Idioma: |
Inglês |
Conteúdo: |
In length heterogeneity PCR (LH-PCR) a fluorescently labeled primer is used to determine the relative amounts of amplified originating from different microorganisms. Labeled fragments are separated by gel electrophoresis and detected by laser-induced fluorescence with an automated gene sequencer. We used LH-PCR to evaluate the composition of the soil microbial community. Four soils, which differed in terms of soil type and/or crop management practice, were studied. Previous data for microbial biomass, nitrogen and carbon contents, and nitrogen mineralization rates suggested that the microbial characteristics of these soils were different. One site received two different treatments: no-till and conventional till perennial ryegrass. The other sites were no-till continuous grass plots at separate locations with different soil types. Community composition was characterized by assessing the natural length heterogeneity in eubacterial sequences amplified from the 5' domain of the 16S rRNA gene and by determining fatty acid methyl ester (FAME) profiles. We found that LH-PCR results were reproducible. Both methods distinguished the three sites. The most abundant bacterial community members, based on cloned LH-PCR products, were members of the B subclass of the class Proteobacteria, the Cytophaga-Flexibacter-Bacteriodes group, and the high-G+C-content gram-positive bacterial group. Strong correlations were found between LH-PCR results and FAME results. We found that the LH-PCR method is an efficient, reliable, and highly reproducible method that should be a useful tool in future assessments of microbial community composition. MenosIn length heterogeneity PCR (LH-PCR) a fluorescently labeled primer is used to determine the relative amounts of amplified originating from different microorganisms. Labeled fragments are separated by gel electrophoresis and detected by laser-induced fluorescence with an automated gene sequencer. We used LH-PCR to evaluate the composition of the soil microbial community. Four soils, which differed in terms of soil type and/or crop management practice, were studied. Previous data for microbial biomass, nitrogen and carbon contents, and nitrogen mineralization rates suggested that the microbial characteristics of these soils were different. One site received two different treatments: no-till and conventional till perennial ryegrass. The other sites were no-till continuous grass plots at separate locations with different soil types. Community composition was characterized by assessing the natural length heterogeneity in eubacterial sequences amplified from the 5' domain of the 16S rRNA gene and by determining fatty acid methyl ester (FAME) profiles. We found that LH-PCR results were reproducible. Both methods distinguished the three sites. The most abundant bacterial community members, based on cloned LH-PCR products, were members of the B subclass of the class Proteobacteria, the Cytophaga-Flexibacter-Bacteriodes group, and the high-G+C-content gram-positive bacterial group. Strong correlations were found between LH-PCR results and FAME results. We found that the LH-PCR method... Mostrar Tudo |
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LEADER 02152naa a2200157 a 4500 001 1621188 005 2001-01-05 008 2000 bl --- 0-- u #d 100 1 $aRITCHIE, N. J. 245 $aUse of length heterogeneity PCR and fatty acid methyl ester profiles to characterize microbial communities in soil. 260 $c2000 520 $aIn length heterogeneity PCR (LH-PCR) a fluorescently labeled primer is used to determine the relative amounts of amplified originating from different microorganisms. Labeled fragments are separated by gel electrophoresis and detected by laser-induced fluorescence with an automated gene sequencer. We used LH-PCR to evaluate the composition of the soil microbial community. Four soils, which differed in terms of soil type and/or crop management practice, were studied. Previous data for microbial biomass, nitrogen and carbon contents, and nitrogen mineralization rates suggested that the microbial characteristics of these soils were different. One site received two different treatments: no-till and conventional till perennial ryegrass. The other sites were no-till continuous grass plots at separate locations with different soil types. Community composition was characterized by assessing the natural length heterogeneity in eubacterial sequences amplified from the 5' domain of the 16S rRNA gene and by determining fatty acid methyl ester (FAME) profiles. We found that LH-PCR results were reproducible. Both methods distinguished the three sites. The most abundant bacterial community members, based on cloned LH-PCR products, were members of the B subclass of the class Proteobacteria, the Cytophaga-Flexibacter-Bacteriodes group, and the high-G+C-content gram-positive bacterial group. Strong correlations were found between LH-PCR results and FAME results. We found that the LH-PCR method is an efficient, reliable, and highly reproducible method that should be a useful tool in future assessments of microbial community composition. 700 1 $aSCHUTTER, M. E. 700 1 $aDICK, R. P. 700 1 $aMYROLD, D. D. 773 $tApplied and Environmental Microbiology, Washington$gv. 66, p. 1668-1675, 2000.
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Embrapa Agrobiologia (CNPAB) |
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Registro Completo
Biblioteca(s): |
Embrapa Agroenergia. |
Data corrente: |
24/08/2016 |
Data da última atualização: |
11/09/2017 |
Autoria: |
MUTADIUIA, C. A. P.; DENARDIN, J. E.; MIRANDA, C. H. B.; SILVA, H. R. da; SILVA FILHO, P. M. da; BARBOSA, G. F.; FERREIRA, G. B.; CRUZ, I.; CARVALHO, M. da C. S.; ROCHA, M. de M.; NEUMAIER, N.; ALMEIDA, R. P. de; SUALEI, F. J.; PIRES, J. L. F.; MUSSA, V. |
Afiliação: |
C. A. P. MUTADIUIA, PNUD; JOSE ELOIR DENARDIN, CNPT; CESAR HERACLIDES BEHLING MIRANDA, CNPAE; HENOQUE RIBEIRO DA SILVA, SRI; PEDRO MOREIRA DA SILVA FILHO, CNPSO; G. F. BARBOSA; GILVAN BARBOSA FERREIRA, CNPA; IVAN CRUZ, CNPMS; MARIA DA CONCEICAO SANTANA CARVALHO, CNPAF; MAURISRAEL DE MOURA ROCHA, CPAMN; NORMAN NEUMAIER, CNPSO; RAUL PORFIRIO DE ALMEIDA, CNPA; F. J. SUALEI, IIAM; JOAO LEONARDO FERNANDES PIRES, CNPT; V. MUSSA, IIAM. |
Título: |
Ensaios com trigo no ProSavana. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
In: SEMINÁRIO DE DIVULGAÇÃO DE RESULTADOS DA INVESTIGAÇÃO AGRÁRIA NO CORREDOR DE NACALA, 2., 2015, Lichinga, Moçambique. Anais... Lichinga, Moçambique: Instituto de Investigação Agrária de Moçambique, 2015. |
Páginas: |
p. 128-137. |
Idioma: |
Português |
Palavras-Chave: |
Agricultura de conservação; ProSavana. |
Thesagro: |
Trigo. |
Categoria do assunto: |
-- |
Marc: |
LEADER 01029nam a2200313 a 4500 001 2051613 005 2017-09-11 008 2015 bl uuuu u00u1 u #d 100 1 $aMUTADIUIA, C. A. P. 245 $aEnsaios com trigo no ProSavana.$h[electronic resource] 260 $aIn: SEMINÁRIO DE DIVULGAÇÃO DE RESULTADOS DA INVESTIGAÇÃO AGRÁRIA NO CORREDOR DE NACALA, 2., 2015, Lichinga, Moçambique. Anais... Lichinga, Moçambique: Instituto de Investigação Agrária de Moçambique$c2015 300 $ap. 128-137. 650 $aTrigo 653 $aAgricultura de conservação 653 $aProSavana 700 1 $aDENARDIN, J. E. 700 1 $aMIRANDA, C. H. B. 700 1 $aSILVA, H. R. da 700 1 $aSILVA FILHO, P. M. da 700 1 $aBARBOSA, G. F. 700 1 $aFERREIRA, G. B. 700 1 $aCRUZ, I. 700 1 $aCARVALHO, M. da C. S. 700 1 $aROCHA, M. de M. 700 1 $aNEUMAIER, N. 700 1 $aALMEIDA, R. P. de 700 1 $aSUALEI, F. J. 700 1 $aPIRES, J. L. F. 700 1 $aMUSSA, V.
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