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Registro Completo |
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
21/11/2017 |
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Data da última atualização: |
29/12/2017 |
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Autoria: |
ABDALLA, S. L.; COSTA, S. S.; GIOSO, M. A.; CASANOVA, L. M.; COUTINHO, M. A. S.; SILVA, M. F. A.; BOTELHO, M. C. da S. N.; DIAS, R. S. G. |
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Afiliação: |
Samira L. Abdalla, Departamento de Cirurgia/Faculdade de Medicina Veterinária e Zootecnia/Universidade de São Paulo -USP; Sônia S. Costa, Instituto de Pesquisas de Produtos Naturais/Universidade Federal do Rio de Janeiro - UFRJ; Marco Antônio Gioso, Departamento de Cirurgia/Faculdade de Medicina Veterinária e Zootecnia/Universidade de São Paulo -USP; Livia M. Casanova, Instituto de Pesquisas de Produtos Naturais/Universidade Federal do Rio de Janeiro - UFRJ; Marcela A. S. Coutinho, Instituto de Pesquisas de Produtos Naturais/Universidade Federal do Rio de Janeiro - UFRJ; Marta Fernanda A. Silva, Departamento de Medicina e Cirurgia/Universidade Federal Rural do Rio de Janeiro - UFRRJ; Maria Clara da S. N. Botelho, Departamento de Parasitologia Animal/UFRRJ; Raquel S. G. Dias, Programa de Ciência Cardiovascular/Faculdade de Medicina/Universidade Federal Fluminense - UFF. |
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Título: |
Efficacy of a Kalanchoe gastonis-bonnieri extract to control bacterial biofilms and dental calculus in dogs. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 37, n. 8, p. 859-865, ago. 2017. |
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Idioma: |
Inglês |
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Notas: |
Título em português: Eficácia do extrato de Kalanchoe gastonis-bonnieri no controle do biofilme bacteriano e cálculo dentário em cães. |
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Conteúdo: |
An aqueous leaf extract of the medicinal species Kalanchoe gastonis-bonnieri (here denominated KGB) has been found to be effective as an antimicrobial agent against canine oral cavity bacteria in in vitro assays. In this study, we investigated the effect of topic oral administration of KGB on the development of dental biofilm in Beagle dogs. The experiments were performed with an experimental group (0.2% of KGB extract), a negative control group (0.9% of saline solution) and a positive control group (0.12% chlorhexidine). Each treatment was sprayed into the oral cavity daily for 28 days. Thirty Beagle dogs with similar characteristics and kept under the same management and diet were used. The measurement of dental plaque and calculus was performed using a computerized analytical method. The phenolic profile of KGB extract was analyzed by HPLC-DAD. KGB extract at 0.2% showed efficacy in controlling the formation of plaque compared to the negative control group, and dental calculus in relation to the negative and positive control groups. A significant difference was observed among these three groups. Peaks attributed to flavonoids and phenolic acids were identified in the HPLC-DAD chromatogram of the KGB extract. The presence of these substances could be related to the activity observed. Our findings demonstrate that treatment with KGB is effective in controlling periodontal disease in dogs, providing new insights into the medicinal properties of this plant. KGB extract has a potential use as a supplemental agent in pharmaceutical products for the prevention of periodontal disease. MenosAn aqueous leaf extract of the medicinal species Kalanchoe gastonis-bonnieri (here denominated KGB) has been found to be effective as an antimicrobial agent against canine oral cavity bacteria in in vitro assays. In this study, we investigated the effect of topic oral administration of KGB on the development of dental biofilm in Beagle dogs. The experiments were performed with an experimental group (0.2% of KGB extract), a negative control group (0.9% of saline solution) and a positive control group (0.12% chlorhexidine). Each treatment was sprayed into the oral cavity daily for 28 days. Thirty Beagle dogs with similar characteristics and kept under the same management and diet were used. The measurement of dental plaque and calculus was performed using a computerized analytical method. The phenolic profile of KGB extract was analyzed by HPLC-DAD. KGB extract at 0.2% showed efficacy in controlling the formation of plaque compared to the negative control group, and dental calculus in relation to the negative and positive control groups. A significant difference was observed among these three groups. Peaks attributed to flavonoids and phenolic acids were identified in the HPLC-DAD chromatogram of the KGB extract. The presence of these substances could be related to the activity observed. Our findings demonstrate that treatment with KGB is effective in controlling periodontal disease in dogs, providing new insights into the medicinal properties of this plant. KGB extract has a ... Mostrar Tudo |
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Palavras-Chave: |
Bacterial biofilm; Biofilme bacteriano; Cálculo dentário; Dental plaque; Doença periodontal; Extrato de planta; Periodontal disease; Placa bacteriana; Plant Extract. |
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Thesagro: |
Cão; Flavonoide. |
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Thesaurus Nal: |
Dental calculus; Dogs; Flavonoids. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/167069/1/Efficacy-of-kalanchoe-gastonis-bonnieri.pdf
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Marc: |
LEADER 02864naa a2200385 a 4500
001 2080206
005 2017-12-29
008 2017 bl uuuu u00u1 u #d
100 1 $aABDALLA, S. L.
245 $aEfficacy of a Kalanchoe gastonis-bonnieri extract to control bacterial biofilms and dental calculus in dogs.$h[electronic resource]
260 $c2017
500 $aTítulo em português: Eficácia do extrato de Kalanchoe gastonis-bonnieri no controle do biofilme bacteriano e cálculo dentário em cães.
520 $aAn aqueous leaf extract of the medicinal species Kalanchoe gastonis-bonnieri (here denominated KGB) has been found to be effective as an antimicrobial agent against canine oral cavity bacteria in in vitro assays. In this study, we investigated the effect of topic oral administration of KGB on the development of dental biofilm in Beagle dogs. The experiments were performed with an experimental group (0.2% of KGB extract), a negative control group (0.9% of saline solution) and a positive control group (0.12% chlorhexidine). Each treatment was sprayed into the oral cavity daily for 28 days. Thirty Beagle dogs with similar characteristics and kept under the same management and diet were used. The measurement of dental plaque and calculus was performed using a computerized analytical method. The phenolic profile of KGB extract was analyzed by HPLC-DAD. KGB extract at 0.2% showed efficacy in controlling the formation of plaque compared to the negative control group, and dental calculus in relation to the negative and positive control groups. A significant difference was observed among these three groups. Peaks attributed to flavonoids and phenolic acids were identified in the HPLC-DAD chromatogram of the KGB extract. The presence of these substances could be related to the activity observed. Our findings demonstrate that treatment with KGB is effective in controlling periodontal disease in dogs, providing new insights into the medicinal properties of this plant. KGB extract has a potential use as a supplemental agent in pharmaceutical products for the prevention of periodontal disease.
650 $aDental calculus
650 $aDogs
650 $aFlavonoids
650 $aCão
650 $aFlavonoide
653 $aBacterial biofilm
653 $aBiofilme bacteriano
653 $aCálculo dentário
653 $aDental plaque
653 $aDoença periodontal
653 $aExtrato de planta
653 $aPeriodontal disease
653 $aPlaca bacteriana
653 $aPlant Extract
700 1 $aCOSTA, S. S.
700 1 $aGIOSO, M. A.
700 1 $aCASANOVA, L. M.
700 1 $aCOUTINHO, M. A. S.
700 1 $aSILVA, M. F. A.
700 1 $aBOTELHO, M. C. da S. N.
700 1 $aDIAS, R. S. G.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 37, n. 8, p. 859-865, ago. 2017.
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Registro original: |
Embrapa Unidades Centrais (AI-SEDE) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
19/04/1999 |
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Data da última atualização: |
19/04/1999 |
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Autoria: |
VLASSAK, K. M.; MERCANTE, F.; STRALIOTTO, R.; FRANCO, A. A.; VUYLSTEKE, M.; VANDERLEYDEN, J. |
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Título: |
Evaluation of the intrinsic competitiveness and saprophytic competence of Rhizobium tropici IIB strains. |
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Ano de publicação: |
1997 |
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Fonte/Imprenta: |
Biology and Fertility of Soils, Berlin, v. 24, p. 274-282, 1997. |
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Idioma: |
Inglês |
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Conteúdo: |
Inoculation of beans (Phaseolus vulgaris L.) with strains of R. tropici IIB and R. etli resulted in the disappearance of the R. tropici IIB stains from the nodule population and their replacement by other (non R. tropici IIB) bean symbionts (Vlassak et al. 1996). Coinoculation studies in monoxenic conditions and in soil core microcosms with plants harvested at two different growth stages indicated that the inoculated R. tropici IIB strains CIAT899 and F98.5 posses a good intrinsic competitiveness which declines, however, at a later plant growth stage and in soil conditions. The poor saprophytic competence of R. tropici IIB strain CIAT899 was further demonstrated by its poor survival in soil core microcosms after bean harvest. Strains were isolated from the field plots with a 3-year bean-planting history, characterized and evaluated for their competitiveness against R. tropici IIB strain CIAT899. Isolates from field plots, which had been repeatedly inoculated with R. tropici IIB strain CIAT899, showed a higher nodule occupancy compared to R. tropici IIB strain CIAT899, and this higher competitiveness exhibited by the field isolates might be an additional reason for the poor performance of R. tropici IIB strain CIAT899 in the field study. Plots with and without a history of bean production revealed after 3-year bean cultivation an almost totally different population that also significantly differed in competitiveness. |
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Palavras-Chave: |
Biological competition; BNF; FBN; Fixação biologica de nitrogênio; Nitrogen fixing bactéria; Saprofita; Saprophytism. |
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Thesagro: |
Competição Biológica; Genética; Nódulo; Solo. |
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Thesaurus NAL: |
genetics; Rhizobium etli; Rhizobium tropici; root nodules; soil. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02466naa a2200373 a 4500
001 1621023
005 1999-04-19
008 1997 bl uuuu u00u1 u #d
100 1 $aVLASSAK, K. M.
245 $aEvaluation of the intrinsic competitiveness and saprophytic competence of Rhizobium tropici IIB strains.
260 $c1997
520 $aInoculation of beans (Phaseolus vulgaris L.) with strains of R. tropici IIB and R. etli resulted in the disappearance of the R. tropici IIB stains from the nodule population and their replacement by other (non R. tropici IIB) bean symbionts (Vlassak et al. 1996). Coinoculation studies in monoxenic conditions and in soil core microcosms with plants harvested at two different growth stages indicated that the inoculated R. tropici IIB strains CIAT899 and F98.5 posses a good intrinsic competitiveness which declines, however, at a later plant growth stage and in soil conditions. The poor saprophytic competence of R. tropici IIB strain CIAT899 was further demonstrated by its poor survival in soil core microcosms after bean harvest. Strains were isolated from the field plots with a 3-year bean-planting history, characterized and evaluated for their competitiveness against R. tropici IIB strain CIAT899. Isolates from field plots, which had been repeatedly inoculated with R. tropici IIB strain CIAT899, showed a higher nodule occupancy compared to R. tropici IIB strain CIAT899, and this higher competitiveness exhibited by the field isolates might be an additional reason for the poor performance of R. tropici IIB strain CIAT899 in the field study. Plots with and without a history of bean production revealed after 3-year bean cultivation an almost totally different population that also significantly differed in competitiveness.
650 $agenetics
650 $aRhizobium etli
650 $aRhizobium tropici
650 $aroot nodules
650 $asoil
650 $aCompetição Biológica
650 $aGenética
650 $aNódulo
650 $aSolo
653 $aBiological competition
653 $aBNF
653 $aFBN
653 $aFixação biologica de nitrogênio
653 $aNitrogen fixing bactéria
653 $aSaprofita
653 $aSaprophytism
700 1 $aMERCANTE, F.
700 1 $aSTRALIOTTO, R.
700 1 $aFRANCO, A. A.
700 1 $aVUYLSTEKE, M.
700 1 $aVANDERLEYDEN, J.
773 $tBiology and Fertility of Soils, Berlin$gv. 24, p. 274-282, 1997.
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Embrapa Agrobiologia (CNPAB) |
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